Abstract
Salmonella typhimurium YG1024 is a derivative of S. typhimurium TA98 with a high level of N-hydroxyarylamine O-acetyltransferase (OAT) activity. We have demonstrated that this strain is highly sensitive to the mutagenic actions of N-hydroxyarylamines derived from aromatic amines and nitroarenes. In this paper, we compared the sensitivities of YG1024 with those of S. typhimurium YG1012, which has about 4 times higher OAT activity than YG1024 but lacks plasmid pKM101. It turned out that YG1024 was more sensitive to the mutagenic actions of 1-aminonaphthalene, 1-nitropyrene, 1,8-dinitropyrene and 2-nitronaphthalene than YG1012 and showed comparable sensitivity to 2-hydroxy-acetylaminofluorene, 2-aminoanthracene and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) to YG1012. These results suggested that YG1024 is more suitable than YG1012 for the efficient detection of mutagenic aromatic amines and nitroarenes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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1-Naphthylamine / toxicity
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4-Nitroquinoline-1-oxide / toxicity
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Acetyltransferases*
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Acyltransferases / metabolism
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Amines / toxicity*
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Anthracenes / toxicity
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Cloning, Molecular
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Environmental Pollutants / toxicity
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Frameshift Mutation
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Hydroxyacetylaminofluorene / toxicity
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Hydroxylamines / toxicity*
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Imidazoles / toxicity
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Microbial Sensitivity Tests
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Mutagenicity Tests*
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Mutagens*
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Naphthalenes / toxicity
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Nitro Compounds / toxicity*
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Plasmids
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Pyrenes / toxicity
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Salmonella typhimurium / drug effects*
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Salmonella typhimurium / enzymology
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Salmonella typhimurium / genetics*
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Species Specificity
Substances
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Amines
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Anthracenes
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Environmental Pollutants
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Hydroxylamines
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Imidazoles
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Mutagens
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Naphthalenes
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Nitro Compounds
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Pyrenes
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1,8-dinitropyrene
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Hydroxyacetylaminofluorene
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4-Nitroquinoline-1-oxide
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2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole
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2-anthramine
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1-Naphthylamine
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Acyltransferases
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Acetyltransferases
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N-hydroxyarylamine O-acetyltransferase
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1-nitropyrene
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2-nitronaphthalene