Comparison of the sensitivity of Salmonella typhimurium strains YG1024 and YG1012 for detecting the mutagenicity of aromatic amines and nitroarenes

Mutat Res. 1993 Jan;301(1):7-12. doi: 10.1016/0165-7992(93)90049-2.

Abstract

Salmonella typhimurium YG1024 is a derivative of S. typhimurium TA98 with a high level of N-hydroxyarylamine O-acetyltransferase (OAT) activity. We have demonstrated that this strain is highly sensitive to the mutagenic actions of N-hydroxyarylamines derived from aromatic amines and nitroarenes. In this paper, we compared the sensitivities of YG1024 with those of S. typhimurium YG1012, which has about 4 times higher OAT activity than YG1024 but lacks plasmid pKM101. It turned out that YG1024 was more sensitive to the mutagenic actions of 1-aminonaphthalene, 1-nitropyrene, 1,8-dinitropyrene and 2-nitronaphthalene than YG1012 and showed comparable sensitivity to 2-hydroxy-acetylaminofluorene, 2-aminoanthracene and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) to YG1012. These results suggested that YG1024 is more suitable than YG1012 for the efficient detection of mutagenic aromatic amines and nitroarenes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Naphthylamine / toxicity
  • 4-Nitroquinoline-1-oxide / toxicity
  • Acetyltransferases*
  • Acyltransferases / metabolism
  • Amines / toxicity*
  • Anthracenes / toxicity
  • Cloning, Molecular
  • Environmental Pollutants / toxicity
  • Frameshift Mutation
  • Hydroxyacetylaminofluorene / toxicity
  • Hydroxylamines / toxicity*
  • Imidazoles / toxicity
  • Microbial Sensitivity Tests
  • Mutagenicity Tests*
  • Mutagens*
  • Naphthalenes / toxicity
  • Nitro Compounds / toxicity*
  • Plasmids
  • Pyrenes / toxicity
  • Salmonella typhimurium / drug effects*
  • Salmonella typhimurium / enzymology
  • Salmonella typhimurium / genetics*
  • Species Specificity

Substances

  • Amines
  • Anthracenes
  • Environmental Pollutants
  • Hydroxylamines
  • Imidazoles
  • Mutagens
  • Naphthalenes
  • Nitro Compounds
  • Pyrenes
  • 1,8-dinitropyrene
  • Hydroxyacetylaminofluorene
  • 4-Nitroquinoline-1-oxide
  • 2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole
  • 2-anthramine
  • 1-Naphthylamine
  • Acyltransferases
  • Acetyltransferases
  • N-hydroxyarylamine O-acetyltransferase
  • 1-nitropyrene
  • 2-nitronaphthalene