Abstract
OBJECTIVE:
To investigate the effect of viral inoculation by adenovirus 5 (Ad5) on body composition in a mouse model.
DESIGN:
Longitudinal monitoring before and after a single injection of virus or saline.
SUBJECTS:
Two groups of CD1 mice, one group given a single intraperitoneal dose of Ad5 and the control group, saline.
MEASUREMENTS:
Bodyweights and food intake were recorded before and up to 21 weeks after inoculation. At the end of the study, whole-body 1H magnetic resonance spectroscopy (MRS) and localised in vivo 1H MRS spectroscopy of the liver was performed to assess whole-body adiposity and intrahepatic lipid content, respectively.
RESULTS:
Ad5-treated animals gained significantly more weight over a period of 21 weeks after inoculation than the controls, 21.8 g (18.8–25.0) and 18.8 g (17.3–19.8) respectively, (P<0.05). The gain in bodyweight in the former animals arises from increased deposition of adipose tissue as measured by whole-body 1H MRS. Adiposity was 6.7% (3.10–11.20%), and 2.40% (0.85–5.65%) for the Ad5-treated and control animals, respectively (P<0.05). No significant difference in intrahepatic lipid content or food intake was observed between the two groups.
CONCLUSION:
The significantly higher percentage of adipose tissue in the Ad5-treated mice suggest viral infection may play a contributory role to a predisposition to obesity, although its contribution relative to other factors remains to be determined.
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References
Sclafani A . Animal models of obesity: classification and characterisation. Int J Obes 1984; 8: 491–508.
Lyons MJ, Faust IM, Hemmes RB, Buskirk DR, Hirsch J, Zabriskie JB . A virally induced obesity in syndrome in mice. Science 1982; 216: 82–85.
Bernard A, Zwingelstein G, Meister R, Wild WT . Hyperinsulineamia induced by canine distemper virus infection of mice and its correlation with the presence of obesity. Comp Biochem Physiol 1988; 91B: 691–696.
Bernard A, Fevre-Montagne M, Giraudon P, Hardin H, Fabian WT, Belin MF . Localisation of viral proteins and RNA in hypothalamus of mice infected by canine distemper virus. Virology 1991; 313: 545–551.
Bernard A, Fevre-Montagne M, Bencsik A, Giraudon P, Wild TF, Confavreux C, Belin MF . Brain structures selectively targeted by canine distemper virus in a mouse model infection. J Neuropathol Exp Neurol 1993; 52: 471–480.
Carter JK, Ow CL, Smith RE . Rous-associated virus type 7 induces a syndrome in chickens characterized by stunting and obesity. Infect Immun 1983; 39: 410–422.
Carter JK, Garlich JD, Donaldson WT, Smith RE . Influence of diet on retrovirus induced obesity and stunting syndrome. Avian Dis 1983; 27: 317–322.
Gosztonyi G, Ludwig H . Borna disease: neuropathology and pathogenesis. Curr Top Microbiol Immunol 1995; 190: 39–73.
Dhurandhar NV, Kulkarni PR, Ajinkya SM, Sherikar AA . Avian adenovirus leading to pathognomic obesity in chickens. J Bombay Vet College 1990; 2: 131–132.
Dhurandhar NV, Kulkarni PR, Ajinkya SM, Sherikar AA . Effect of adenovirus infection on adiposity in chickens. Vet Microbiol 1992; 31: 101–107.
Dhurandhar NV, Israel BA, Kolesar JM, Mayhew GF, Cook ME, Atkinson RL . Increased adiposity in animals due to a human virus. Int J Obes Relat Metab Disord 2000; 24: 989–996.
Dhurandhar NV, Israel BA, Kolesar JM, Mayhew GF, Cook ME, Atkinson RL . Transmissibility of adenovirus-induced adiposity in a chicken model. Int J Obes Relat Metab Disord 2001; 25: 990–996.
Dhurandhar NV, Whigham LD, Abbott DH, Schultz-Darken NJ, Israel BA, Bradley SM, Kemnitz JW, Allison DB, Atkinson RL . Human adenovirus Ad-36 promotes obesity in male rhesus and marmoset monkeys. J Nutr 2002; 132: 3155–3160.
Dhurandhar NV, Kulkarni PR, Ajinkya SM, Sherikar AA, Atkinson RL . Screening of human sera for antibody against avian adenovirus. Obes Res 1997; 5: 464–469.
Atkinson RL, Dhurandhar NV, Allison DB, Bowen R, Israel BA . Evidence for an association of an obesity virus with human obesity at three sites in the United States. Int J Obes Relat Metab Disord 1998; 22: S57.
Atkinson RL, Dhurandhar NV . From cells to organisms: new thoughts on the etiology of obesity. In: Ntambi (ed). Adipocyte biology and signalling. IOS Press: Amsterdam; 2000. pp 165–174.
Wilson JM . Adenoviruses as gene delivery vehicles. N Engl J Med 1996; 334: 1185–1187.
Mystkowski P, Shankland E, Schreyer SA, LeBoeuf RC, Schwartz RS, Cummings DE, Kushmerick M, Schwartz MW . Validation of whole-body magnetic resonance spectroscopy as a tool to assess murine body composition. Int J Obes Relat Metab Disord 2000; 24: 719–724.
Pierson RN, Wand J, Thornton JC . Measurement of body composition: applications in hormone research. Horm Res 1997; 48: 56–62.
Vangipuram SD, Sheele J, Atkinson RL, Holland TC, Dhurandhar NV . A human adenovirus enhances preadipocyte differentiation. Obes Res 2004; 12: 770–777.
Clark RB . The role of PPARs in inflammation and immunity. J Leuc Biol 2002; 71: 388–400.
Nakajima A, Wada K, Miki H, Kubota N, Nakajima N, Terauchi Y, Ohnishi S, Saubermann LJ, Kadowaki T, Blumberg RS, Nagai R, Matsuhashi N . Endogenous PPAR gamma mediates anti-inflammatory activity in murine ischemia-reperfusion injury. Gastroenterology 2001; 120: 460–469.
Youssef J, Badr M . Role of peroxisome proliferator-activated receptors in inflammation control. J Biomed Biotechnol 2004; 2004: 156–166.
Acknowledgements
We thank Dr I McNeish for the gift of adenovirus 5, The Wellcome Trust for funding the Biological Imaging Centre facility, the Medical Research Council for funding the research and Ernesto Torres-Guzman por inspiratio.
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So, PW., Herlihy, A. & Bell, J. Adiposity induced by adenovirus 5 inoculation. Int J Obes 29, 603–606 (2005). https://doi.org/10.1038/sj.ijo.0802917
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DOI: https://doi.org/10.1038/sj.ijo.0802917
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