Original ContributionUrinary excretion of biomarkers of oxidatively damaged DNA and RNA in hereditary hemochromatosis
Section snippets
Material and methods
The study design has been described in detail elsewhere [1]. In brief, urine samples were collected from 21 newly diagnosed untreated cases with hereditary hemochromatosis and 21 controls, and blood samples were secured for analysis of iron status. Liver iron concentration and non-transferrin-bound iron were quantified in patients only [1]. All patients were HFE C282Y homozygotes. Spot urine samples were collected from controls and from the patients before the latter underwent treatment with
Results
At baseline the urinary excretion of the RNA oxidation product 8-oxoGuo was 2.5-fold increased in patients with hereditary hemochromatosis (P = 0.006) compared with controls (Table 1). There were no significant differences in the excretion of the DNA oxidation product (8-oxodGuo) or the oxidized base (8-oxoGua).
After treatment of the patients with phlebotomy, the excretion of both the DNA and the RNA oxidation products was reduced significantly (Table 2). The excretion of the RNA oxidation
Discussion
In this study we show a 2.5-fold increase in RNA oxidation, measured as excretion of 8-oxoGuo in HFE C282Y homozygous patients compared with controls. Interestingly, the DNA oxidation measured as excretion of 8-oxodGuo did not differ between hemochromatosis patients and controls. However, after treatment of the hemochromatosis patients until normalization of their iron parameters, both RNA and DNA oxidation were reduced—the DNA oxidation by 30% and the RNA oxidation to one-third of the values
Acknowledgment
This study was supported by research funding from the Research Committee at Copenhagen University Hospital–Rigshospitalet.
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