Scientific paperThe effects of duodenal-jejunal exclusion on hormonal regulation of glucose metabolism in Goto-Kakizaki rats
Section snippets
Animals and diet
Twelve male Goto-Kakizaki rats who were 10 to 12 weeks of age were purchased from Taconic M&B A/S (Copenhagen, Denmark). Male rats were used to avoid hypothetical ovarian cycle effects on enteroinsular hormone levels. Animals had free access to tap water and were fed with 20 to 25 g per day of 5% fat rat chow diet (Altromin 04; Altromin, Gesellschaft, Germany) during all the protocol. The study was approved by Investigation Commette of the Hospital.
Experimental protocol
After the rats were aclimated for 2 weeks,
Results
Before treatment, there were no significant differences between groups in terms of weight (group 1: 259 ± 45.6 g versus group 2: 263.3 ± 26.4 g, not significant) or fasting glycemia (group 1: 260.6 ± 128 mg/dL versus group 2: 249.4 ± 47.7 mg/dL, not significant). In group 1, weight did not show intragroup differences during follow-up (basal 249.4 ± 47.7 g versus 1 week 290.3 ± 45 g versus 1 month 314.6 ± 40 g, not significant). In group 2, weight was similar during the study (basal 263.3 ± 26.4
Comments
Our data show that the bypass of the duodenum and proximal jejunum reduces fasting glycemia and improves glucose tolerance test, with a significant paradoxical decrease of leptin levels after surgery.
The first consideration of our study is that the control of diabetes mellitus induced by duodenal-jejunal exclusion is not dependent on the weight reduction because weight of rats remained unchanged and our model is a nonobese model [10]. In our study, the effect on glucose metabolism seems to be a
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