NMR-based metabonomic toxicity classification: hierarchical cluster analysis and k-nearest-neighbour approaches
Introduction
In the pharmaceutical industry the early identification of toxicological side-effects of potential new drug candidates is of fundamental value in the minimisation of compound “attrition” [1]. While specific assays have been developed to detect abnormal changes in biofluids of test animals, histopathological tissue examination remains the ‘gold-standard’ in toxicological assessment. Since investigating toxicity by traditional techniques is time consuming and costly, different approaches to the reliable and effective prediction of toxicity have been explored. Currently, there are several advanced technologies using multiparametric biochemical information derived from different levels of biomolecular organisation. Genomic and proteomic information describing transcriptional effects and protein synthesis can be extracted [2], [3]. However, these approaches are also slow and expensive. Alternatively, the metabonomic approach analyses the entire pool of endogenous metabolites in a biofluid or tissue, and is a powerful technique for detecting and describing biological endpoint-effects. It has already proven to be effective in the description of genetic strain differences, disease states, nutritional effects and toxic influences [1], [4], [5], [6], [7], [8], [9]. In this study, we report the initial evaluation of metabonomic data yielded in a large-scale toxicological project within the COnsortium for MEtabonomic Toxicology (COMET). The COMET1 project is currently constructing databases and metabolic models of drug toxicity using ca. 100,000 NMR spectra of biofluids from rats and mice treated with approximately 150 model compounds. Multivariate statistical models characterising the effects of toxins on endogenous metabolite profiles have been generated and will be used for the development of computer-based expert systems. These will enable rapid pre-clinical toxicological screening of potential drug candidates.
As a prototype for such large and comprehensive databases we have used a subset of the data to study dose- and time-related effects, to develop and evaluate classification methods and explore characteristic features of toxicity (the ‘biomarker profile’). This facilitates the prediction and interpretation of large-scale data sets and will help to hone the development of new analytical tools. We describe a first attempt to explore and predict the effect of different toxins, surgical procedures and other external influences in 19 independent studies using NMR-metabonomic analysis of rat urine samples. Urine samples were collected from dosed and control rats at 10 time points over a period of 8 days and were subsequently analysed by 600 MHz NMR spectroscopy. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were applied to the data from the studies to reveal dose and time-related effects in the response of animals to different toxins. Finally, k-nearest-neighbour (kNN) classification [10] was used to predict how well individual samples were classified into their respective class and time point. This work gives an insight into the scope of this project and illustrates the diagnostic power and reliability of metabonomic data analysis using NMR spectroscopy together with chemometric techniques for the exploration and prediction of toxic effects.
Section snippets
Sample collection
In established in-house or external animal research facilities of the involved pharmaceutical companies, male 8–10 week old Sprague-Dawley rats were housed in metabolism cages and urine samples collected daily. Free access to food and water was permitted throughout the studies (except in the case of food and water restriction studies). The animals were randomly assigned to three dose groups: control (vehicle only, n=10), high-dose (dose causing a clear toxic effect, n=10) and low-dose
Overall analysis
PCA of the averaged spectra at each time point gave a clear overview of the treatment- and time-related metabonomic effects as observed by NMR spectroscopy of the urine (Fig. 2). Separation of these data points can be attributed to alterations in NMR signals of endogenous urinary metabolites as seen in the loadings plot. Urines from streptozotocin-treated animals revealed a urinary metabolic profile distinct from all other treatment groups, mainly due to elevated glucose signals (δ = 3.4–3.88).
Conclusions
As part of a larger effort to construct a comprehensive database of urinary metabonomic data from toxicological investigations, these preliminary results have underlined the potential of our approach for the prediction of drug toxicity. Data were employed from animal studies showing metabonomic effects due to toxin administration, surgical procedures (partial hepatectomy, unilateral nephrectomy) and other treatments (food restriction, water deprivation). It was shown that chemometric methods
Acknowledgements
OB, MEB, TMDE and HCK thank the members of the Consortium for Metabonomic Toxicology (Bristol-Myers Squibb, Eli Lilly, Hoffmann-La Roche, Novo Nordisk, Pfizer and Pharmacia) for financial support.
References (17)
- et al.
An NMR-based metabonomic approach to investigate the biochemical consequences of genetic strain differences: application to the C57BL10J and Alpk:ApfCD mouse
FEBS Lett.
(2000) - et al.
NMR spectroscopy based metabonomic studies on the comparative biochemistry of the kidney and urine of the bank vole (Clethrionomys glareolus), wood mouse (Apodemus sylvaticus), white toothed shrew (Crocidura suaveolens) and the laboratory rat
Comp. Biochem. Physiol. Part B Biochem. Mol. Biol.
(2000) - et al.
Investigations into biochemical changes due to diurnal variation and estrus cycle in female rats using high-resolution NMR spectroscopy of urine and pattern recognition
Anal. Biochem.
(2001) - et al.
Microarray analysis of hepatotoxins in vitro reveals a correlation between gene expression profiles and mechanisms of toxicity
Toxicol. Lett.
(2001) - et al.
Clustering of hepatotoxins based on mechanism of toxicity using gene expression profiles
Toxicol. Appl. Pharm.
(2001) - et al.
Metabonomics: a platform for studying drug toxicity and gene function
Nat. Rev. Drug Discov.
(2002) - et al.
The promise of toxicogenomics
Curr. Opin. Mol. Ther.
(2002) The role of proteomics in toxicology: identification of biomarkers of toxicity by protein expression analysis
Biomarkers
(2002)
Cited by (134)
Metabolomics approach in pharmacognosy
2023, Pharmacognosy: Fundamentals, Applications, and Strategies, Second EditionThe most consumed beef cuts in Brazil: Prices versus metabolic profile
2023, Journal of Food Composition and AnalysisComposite score analysis for unsupervised comparison and network visualization of metabolomics data
2020, Analytica Chimica ActaThe effect of temperature on sulfate release from Pearl River sediments in South China
2019, Science of the Total EnvironmentMetabolomics in early detection and prognosis of acute coronary syndrome
2019, Clinica Chimica Acta