Abstract
Purpose
Determine the outcome of embryo cryopreservation in female oncology patients
Methods
The outcomes of IVF/ICSI cycles in oncology patients over 15 years in a University Teaching Hospital.
Results
Forty-two oncology patients (mean 31.9 ± 3.9 years) underwent embryo cryopreservation treatment (n = 33 IVF, n = 6 ICSI). Controlled ovarian stimulation with GnRH antagonist protocol (n = 34; 81 %) yielded fewer oocytes than GnRH agonist protocol (n = 8; 19 %) (9.4 ± 6.3 vs. 15.3 ± 8.9; p = 0.04) respectively. There was no significant difference in mean (±SD) duration of ovarian stimulation (11.6 ± 2.6 vs.10.6 ± 2.7), median gonadotrophin dose (1950 vs. 1670 IU), median day 5–6 oestradiol level (1124 vs.1129 pmol/l) or embryo yield (6.2 ± 4.1 vs. 8.8 ± 4.3; p = 0.07) between GnRH antagonist and agonist treatment cycles respectively. Thirty-nine patients cryopreserved embryos and three had their cycle cancelled. During this study period, of those who cryopreserved embryos, 5 patients underwent 9 frozen-thaw cycles (13 %), resulting in 2 live births (1 twin, 1 singleton, live birth rate 22 %). Six patients died (15 %), 3 conceived naturally (8 %) and 2 couples separated (5 %). Fourteen patients discarded their embryos (36 %). Twenty-two patients’ (56 %) have embryos remaining in storage.
Conclusions
This study demonstrates that embryo cryopreservation in female oncology patients gives a satisfactory live birth rate. However, there are concerns regarding cost-effectiveness, resulting from high disposal/non-usage of embryos, and further studies are required.
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References
Al-Inany H, Aboulghar M. GnRH antagonist in assisted reproduction: a cochrane review. Hum Reprod. 2002;17:874–85.
Al-Inany HG, Youssef MAFM, Aboulghar M, Broekmans F, Sterrenburg M, Smit J, et al. GnRH antagonists are safer than agonists: an update of a Cochrane review. Hum Reprod Update. 2011;17:435.
Azim Jr HA, Peccatori FA, de Azambuja E, Piccart MJ. Motherhood after breast cancer: searching for la dolce vita. Expert Rev Anticanc. 2011;11:287–98.
Baynosa J, Westphal LM, Madrigrano A, Wapnir I. Timing of breast cancer treatments with oocyte retrieval and embryo cryopreservation. J Am Coll Surg. 2009;209:603–7.
Berger BM, Ezcurra D, Alper MM. Gonadotropin releasing hormone (GnRH) antagonist (cetrorelix) versus GnRH agonist treatment—an age and gonadotropin matched study in young normal responders. Fertil Steril. 2004;82:S235–6.
Besse D, Bellavia M, de Ziegler D and Wunder D. Psychological support in young women who contemplate emergency assisted reproductive technologies (ART) prior to chemo- and/or radiation-therapy. Swiss Med Wkly. 2010;140.
Brzyski RG. Efficacy of postal communication with patients who have cryopreserved pre-embryos. Fertil Steril. 1998;70:949–51.
Cobo A, Kuwayama M, Pérez S, Ruiz A, Pellicer A, Remohí J. Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method. Fertil Steril. 2008;89(6):1657–64.
Das M, Shehata F, Moria A, Holzer H, Son W-Y, Tulandi T. Ovarian reserve, response to gonadotropins, and oocyte maturity in women with malignancy. Fertil Steril. 2011;96:122–5.
De Bree E, Makrigiannakis A, Askoxylakis J, Melissas J, Tsiftsis DD. Pregnancy after breast cancer. A comprehensive review. J Surg Oncol. 2010;101:534–42.
Duffy CM, Allen SM, Clark MA. Discussions regarding reproductive health for young women with breast cancer undergoing chemotherapy. J Clin Oncol. 2005;23:766–73.
Edgar DH, Gook DA. A critical appraisal of cryopreservation (slow cooling vs vitrification) of human oocytes and embryos. Hum Reprod Update. 2012;in press.
Fasouliotis SJ, Schenker JG. Cryopreservation of embryos: medical, ethical, and legal issues. J Assist Reprod Gen. 1996;13:756–61.
Forbes JF. Long-term effects of adjuvant chemotherapy in breast-cancer. Acta Oncol. 1992;31:243–50.
Forman EJ, Anders CK, Behera MA. Pilot survey of oncologists regarding treatment-related infertility and fertility preservation in female cancer patients. 2009;54(4):203–7.
Hery C, Ferlay J, Boniol M, Autier P. Changes in breast cancer incidence and mortality in middle-aged and elderly women in 28 countries with Caucasian majority populations. Ann Oncol. 2008;19:1009–18.
Holleb AI. Breast cancer and pregnancy. CA Cancer J Clin. 1965;15:182–3.
Janerich DT. The fetal antigen hypothesis: cancers and beyond. Med Hypotheses. 2001;56:101–3.
Kini S, Li HWR, Morrell D, Pickering S, Thong KJ. Anti-mullerian hormone and cumulative pregnancy outcome in in-vitro fertilization. J Assist Reprod Gen. 2010;27:449–56.
Lohrisch C, Paltiel C, Gelmon K, Speers C, Taylor S, Barnett J, et al. Impact on survival of time from definitive surgery to initiation of adjuvant chemotherapy for early-stage breast cancer. J Clin Oncol. 2006;24:4888–94.
Lee S, Oktay K. Does higher starting dose of FSH stimulation with letrozole improve fertility cryopreservation outcomes in women with breast cancer? Fert Steril. 2012;98(4):961–4.
Nachtigall RD, Mac Dougall K, Lee M, Harrington J, Becker G. What do patients want? Expectations and perceptions of IVF clinic information and support regarding frozen embryo disposition. Fertil Steril. 2010;94:2069–72.
Nagy ZP, Chang CC, Shapiro DB, Bernal DP, Elsner CW, Mitchell-Leef D, et al. Clinical evaluation of the efficiency of an oocyte donation program using egg cryo-banking. Fertil Steril. 2009;92(2):520–6.
Oktay K, Buyuk E, Libertella N, Akar M, Rosenwaks Z. Fertility preservation in breast cancer patients: a prospective controlled comparison of ovarian stimulation with tamoxifen and letrozole for embryo cryopreservation. J Clin Oncol. 2005;23:4347–53.
Provoost V, Pennings G, De Sutter P, Gerris J, Van de Velde A, Dhont M. To continue or discontinue storage of cryopreserved embryos? Patients’ decisions in view of their child wish. Hum Reprod. 2011;26:861–72.
Riggs R, Mayer J, Dowling-Lacey D, Chi T-F, Jones E, Oehninger S. Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human embryos. Fertil Steril. 2010;93:109–15.
Robertson AD, Missmer SA, Ginsburg ES. Embryo yield after in vitro fertilization in women undergoing embryo banking for fertility preservation before chemotherapy. Fertil Steril. 2011;95:588–91.
Sonmezer M, Turkcuoglu I, Coskun U, Oktay K. Random-start controlled ovarian hyperstimulation for emergency fertility preservation in letrozole cycles. Fertil Steril. 2011;95(6):2125.
Stadtmauer LA, Duran H, Bocca S, Oehninger S. A comparison between gonadotropin releasing hormone (GNRH) agonist and antagonist protocols in women with good ovarian reserve undergoing IVF. Fertil Steril. 2006;86:S437.
Tschudin S, Bitzer J. Psychological aspects of fertility preservation in men and women affected by cancer and other life-threatening diseases. Hum Reprod Update. 2009;15:587–97.
Venn A, Watson L, Bruinsma F, Giles G, Healy D. Risk of cancer after use of fertility drugs with in-vitro fertilisation. Lancet. 1999;354:1586–90.
Yang D, Brown SE, Nguyen K, Reddy V, Brubaker C, Winslow KL. Live birth after the transfer of human embryos developed from cryopreserved oocytes harvested before cancer treatment. Fertil Steril. 2007;87(6):1469.
Acknowledgments
Finally, we would like to thank all of the IVF staff at the Edinburgh Reproductive and Endocrine Fertility Centre for their support with this study.
Authors roles
JB contributed to the conception and design, acquisition, analysis and interpretation of data, and drafted the article. JT and ND contributed to conception and design, acquisition of data and were involved in the critical revision of the intellectual content of the manuscript.
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The authors declare that they have no conflict of interest
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Capsule Only a minority of cryopreserved embryos in female oncology patients over a fifteen year period were replaced.
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Barcroft, J., Dayoub, N. & Thong, K.J. Fifteen year follow-up of embryos cryopreserved in cancer patients for fertility preservation. J Assist Reprod Genet 30, 1407–1413 (2013). https://doi.org/10.1007/s10815-013-0024-z
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DOI: https://doi.org/10.1007/s10815-013-0024-z