TableĀ 4

Sequencing of serially diluted DNA isolated from formalin-fixed paraffin-embedded (FFPE) human pancreatic cancer cell lines with known mutation statuses

Panc-1in BxPC-3KRAS p.G12D (c.35G>A)TP53 p.R273H (c.818G>A)
Returned variant frequency (%)Coverage (X)Returned variant frequency (%)Coverage (X)
100% (undiluted)68.01599100286
50% (1:1)45.0164443.6219
20% (1:4)24.0162933.3244
10% (1:9)12.6144815.4269
5% (1:19)6.613956.4273
2% (1:49)4.113884.5220
1% (1:99)2.414142.2285