TableĀ 5

Application of Ion Torrent PGM for extended RAS analysis in formalin-fixed paraffin-embedded (FFPE) colorectal cancer samples

Patient numberGeneExonIon Torrent PGMSanger
Variants
VariantsCoverage (X)Frequency (%)
1KRAS4p.K117N(c.351A>T)99044.4p.K117N(c.351A>T)
7KRAS3p.Q61H(c.183A>C)84734.6p.Q61H(c.183A>C)
12KRAS3p.Q61H(c.183A>T)201245.4p.Q61H(c.183A>T)
17KRAS4p.A146T(c.436G>A)73236.7p.A146T(c.436G>A)
28KRAS3p.Q61L(c.182A>T)114138.8p.Q61L(c.182A>T)
39KRAS4p.K117N(c.351A>T)242042.6p.K117N(c.351A>T)
40KRAS4p.K117N(c.351A>T)46214.3p.K117N(c.351A>T)
49KRAS4p.A146V(c.437C>T)107066.4p.A146V(c.437C>T)
50KRAS4p.A146T(c.436G>A)52658.0p.A146T(c.436G>A)
47NRAS2p.G12C(c.34G>T)50241.4p.G12C(c.34G>T)