Table 1

Overview of additional data collected in LifeLines DEEP, including the number of samples, the source biomaterial it originates from and the method of analysis used

LifeLines DEEP datanSourceMethods
Biological ageing1387Cells from whole bloodFlowFish
Biological ageing1387DNA from whole bloodqPCR and sjTRECs
Biomarkers (citrulline, cytokines)1387PlasmaHPLC, ECLIA
Biomarkers (calprotectin, HBD-2, chromogranin A, SCFA)1248FaecesELISA, ELISA, RIA, GC-MS
CVD risk score1448Biochemical measures and questionnaireScoring algorithm Framingham Heart Study
Functional studies1387PBMC from whole bloodVarious methods
Gastrointestinal symptoms1176QuestionnaireRome III criteria and Bristol Stool Form Scale
Genetics1387DNA from whole bloodCytoSNP and ImmunoChip, GoNL as imputation reference
Metabolomics1425Exhaled airGC-tof-MS
Methylation761+DNA from whole blood450 K chip
Microbiome1248Faeces16S rRNA based sequencing
Transcriptomics1387Whole blood (PAXgene)RNA sequencing
  • CVD, cardiovascular disease; ECLIA, electro-chemiluminescence immunoassay; GC-(tof-)MS, gas chromatography-(time of flight-)mass spectrometry; HBD-2, human β defensin 2; HPLC, high-performance liquid chromatography; NMR, nuclear MR; qPCR, quantitative PCR; RIA, radioimmunoassay; SCFA, short chain fatty acids; sjTRECs, signal joint T cell receptor excision circles.