Summary measures of performance of the direct smear diagnostic tests for 150 matched samples and the statistical differences in sensitivity and specificity of FM, CY and PiLED in comparison with ZN
| Gold stand | Direct Smear | Sn | Sp | PPV | NPV | SnD (95% CI) | p Value* | SpD (95% CI) | p Value* |
|---|---|---|---|---|---|---|---|---|---|
| A | ZN | 50.0 | 89.7 | 81.8 | 66.0 | ||||
| FM | 61.1 | 79.5 | 73.3 | 68.9 | 11.1 (3.0 to 19.0) | 0.027 | 10.2 (2.5 to 17.4) | 0.182 | |
| CY | 54.2 | 87.2 | 79.6 | 67.3 | 4.2 (−2.9 to 11.3) | 0.450 | 2.5 (−3.6 to 8.6) | 0.684 | |
| PiLED | 59.7 | 73.1 | 67.2 | 66.3 | 9.7 (1.9 to 17.5) | 0.046 | 16.6 (8.6 to 24.6) | 0.001 | |
| B | ZN | 35.8 | 78.3 | 65.9 | 50.9 | ||||
| FM | 44.4 | 65.2 | 60.0 | 50.0 | 8.6 (2.4 to 14.8) | 0.023 | 13.1 (3.9 to 22.1) | 0.016 | |
| CY | 40.7 | 76.8 | 67.3 | 52.5 | 4.9 (0.2 to 9.6) | 0.134 | 2.5 (−4.4 to 9.4) | 0.684 | |
| PiLED | 48.1 | 63.8 | 60.9 | 51.2 | 12.3 (4.9 to 19.6) | 0.004 | 1.4 (−5.5 to 7.9) | 0.683 | |
| C | ZN | 45.6 | 84.1 | 70.5 | 65.1 | ||||
| FM | 51.5 | 69.5 | 58.3 | 63.3 | 5.9 (−2.1 to 13.9) | 0.289 | 14.6 (6.6 to 22.6) | 0.006 | |
| CY | 48.5 | 80.5 | 67.3 | 65.3 | 3.1 (−0.9 to 7.1) | 0.479 | 3.6 (−2.6 to 9.8) | 0.131 | |
| PiLED | 57.4 | 69.5 | 60.9 | 66.3 | 11.8 (2.2 to 21.4) | 0.043 | 14.6 (7.5 to 21.7) | 0.004 |
SnD, sensitivity difference; percentage points above ZN performance.
SpD, specificity difference; percentage points below ZN performance.
*χ2 p value generated from McNemar's test.
CY, CysCope fluorescence microscopy; FM, mercury vapour fluorescence microscopy; PiLED, Primo Star iLED fluorescence microscopy; Sn, sensitivity; Sp, specificity; PPV, positive predictive value; NPV, negative predictive value.