Direct smear (DS) | DS Outcome | Gold standards: MGIT cultures | |||||
---|---|---|---|---|---|---|---|
A: culture positive=72 | B: culture positive=81 | C: culture positive=68 | |||||
P | N | P | N | P | N | ||
ZN | P | 36 | 08 | 29 | 15 | 31 | 13 |
DS+ = 44 | N | 36 | 70 | 52 | 54 | 37 | 69 |
FM | P | 44 | 16 | 36 | 24 | 35 | 25 |
DS+ =60 | N | 28 | 62 | 45 | 45 | 33 | 57 |
CY | P | 39 | 10 | 33 | 16 | 33 | 16 |
DS+ =49 | N | 33 | 68 | 48 | 53 | 35 | 66 |
PiLED | P | 43 | 21 | 39 | 25 | 39 | 25 |
DS+ =64 | N | 29 | 57 | 42 | 44 | 29 | 57 |
Culture specimen decontaminated by NaOH–NALC: A for 10 min; B for 15 min; C for 20 minutes.
DS+=number of samples (out of 150 samples) that were AFB positive by direct smear.
AFB, acid-fast bacilli; CY, CysCope fluorescence microscopy; FM, mercury vapour fluorescence microscopy; P, positive; PiLED, Primo Star iLED fluorescence microscopy; N, Negative; ZN, Ziehl Neelsen microscopy.