Measures | Variables | Instrument used where applicable | Baseline | Follow-up |
Anthropometrics | Weight | SECA 877 | x | x |
Height | SECA 217 | x | x | |
Waist circumference | Measuring tape | x | x | |
Hip circumference | Measuring tape | x | x | |
Body fat (bio impedance analysis) | BODYSTAT 1500 MDD analyser for BIA | x | ||
BP, office | Systolic BP, diastolic BP, pulse, measured three times | Microlife BP A6 BT | x | x |
24 hours ABPM In subsample only | 24 hours ABPM | Spacelabs 90207/90217 | x | |
Ankle-Brachial Index | PAD | Ankle-Brachial Index, measured in a supine position after at least 10 min rest | x | |
Biological samples | ||||
On the spot glucose In Ghana only | Nova Xpress meter+StatStrip GLU—test strip | x | x | |
Glucose metabolism | Glucose HbA1C | x | x | |
Lipid metabolism | Total cholesterol | x | x | |
HDL-C | x | x | ||
LDL-C | x | x | ||
Triglycerides | x | x | ||
Inflammation | hsCRP | x | x | |
Uric acid metabolism | Uric acid | x | ||
Oxidative stress | Ferritin | x | ||
Electrolytes and renal function | Creatinine | x | x | |
Albumin | x | |||
Sodium | x | |||
Potassium | x | |||
Calcium | x | |||
Liver metabolism | ALAT | x | x | |
ASAT | x | x | ||
y-GT | x | x | ||
Renin–angiotensin–aldosterone system Subsample only | Renin | x | ||
Aldosterone | x | |||
Adipokines Subsample only | Adiponectin | x | ||
Leptin | x | |||
Non-esterified fatty acid | x | |||
Urine analysis | ||||
Morning void | Albumin | x | x | |
Creatinine | x | x | ||
Sodium | x | x | ||
Potassium | x | x | ||
24 hours urine collection In subsample only | 24 hours albumin | x | ||
24 hours creatinine | x | |||
24 hours sodium | x | |||
24 hours potassium | x | |||
24 hours urea | x | |||
Stool sample | Microbiome | x | ||
(Epi-)Genetics In subsample only | ||||
Epigenetics | DNA methylation | Infinium Methylation EPIC BeadChip of Illumina (850K) | x | x |
RNA expression analysis | x |
ABPM, ambulatory blood pressure measurement; ALAT, alanine transaminase; ASAT, aspartate aminotransferase; BP, blood pressure; HbA1c, hemoglobine A1c; HDL-C, high-density lipoprotein cholesterol; hsCRP, high-sensitivity C reactive protein; LDL-C, low-density lipoprotein cholesterol; PAD, peripheral artery disease; γGT, γ-glutamyl transpeptidase.