Markers of immunosenescence included in the flow cytometry analyses
| No | Marker | Cell type | Gating |
| Viability dye | |||
| 1 | CD3 | T cells | |
| 2 | CD4 | Monocytes, helper T cells | |
| 3 | CD8 | Cytotoxic T cells | |
| 4 | CD19 | B cells | |
| 5 | CD56 | NK cells | CD8+ |
| 6 | CD57 | Senescent and NK subsets | CD27+ CD57+ CD127+ |
| 8 | CD45RA | Treg cells Naïve T reg | |
| 9 | CD25 | ||
| 10 | CD27 | ||
| 11 | CD127 | ||
| 12 | CD28 | Senescent and NK subsets | |
| 13 | CCR7 | Effector memory cells | CD45+ RA |
| 14 | CD16 | Monocytes/dendritic cells | |
| 15 | CD11c | ||
| 16 | HLA DR | ||
| 17 | CD14 | ||
| 18 | CD31 | Thymic migration | CD4+ |
| 19 | CD38 |