Table 1

Methods of bilirubin detection and quality assurance by study location, Young Infant Clinical Signs Study Group

Study locationMethod for bilirubin measurementQuality control measures
(1) Dhaka Shishu Hospital,
Dhaka, Bangladesh
Dimethylysulfoxide (Chronolab AG, Zug, Switzerland) colorimetric method using a Humalyzer—2000 (Human Gesellschatt for Biochemica and Diagnostic mbH, Germany)Quality control was done daily using calibration and quality control serum from the manufacturer
(2) All India Institute of Medical Sciences and Safdarjung Hospital, Delhi, India;
(3) Postgraduate Institute for Medical Education and Research and General Hospital, Sector 16, Chandigarh, India
Spectrophotometer (BIL-100, Cosmo Medical Co., Ltd., Seoul, Korea) with built-in auto-calibrationBilirubin standards were used periodically (every 4–5 months) to cross-check the calibration
(4) King Edward VIII Hospital, Durban, South AfricaColorimetric method using a Roche Modular P-800 spectrophotometer (Roche Modular 800 (Roche Diagnostics, Basel, Switzerland)The machine was manually calibrated whenever a new kit was inserted or when the machine sent out an automated request for calibration, approximately once weekly
(5) Komfo Anokye Teaching Hospital, Kumasi, GhanaAutomated colorimetric method using a spectrophotometric autoanalyser (Atac-8000, Elan Diagnostics, Smithfield, RI, USA)The machine was calibrated daily with the manufacturer’s controls
(6) Hospital del Niño and Hospital Materno-Infantil, La Paz, BoliviaDiazo method (Synchron CX5 Beckman Coulter, Inc, Fullerton, CA, USA)Quality control was run once daily with a known sample provided by the manufacturer