Minimum number of patients needed in each cohort and overall, assuming a 95% confidence level and 5% margin of error for the percentage of cases changed
| Cohort | Expected percentage of cases changed | Number of patients needed in cohort | Percentage of overall sample in cohort | Total sample size needed |
| Stage II patients | 34* | 345 | 45 | 767 |
| Stage III patients | 46* | 382 | 55 | 695 |
| Patients with (−) ctDNA result | 25 | 289 | 77.25 | 375 |
*Based on the highest percentage of cases changed within each stage from table 4.
ctDNA, circulating tumour DNA.