Table 3

Test meal guidelines

Time point guidelinesProcedure
Arrival 08:00*Blood sample taken.
Subjects asked to empty bladder; start of timed 24-hour urine collection.
Weight, waist circumference, bioelectrical impedance analysis, pulse and blood pressure taken.
08:10Basal metabolic rate measurement (indirect calorimetry—see below for details).
08:55 (T1 VAS)Subjects will complete a pre-breakfast, seven-component VAS questionnaire to measure hunger and other subjective variables.
09:00Subjects will be provided with a fixed-quantity breakfast, consisting of cornflakes with milk, toast and preserve, tea/coffee and orange juice.
09:20 (T2 VAS)Subjects will complete a post-breakfast, 7-component VAS questionnaire to measure hunger and other subjective variables.
10:00, 11:00, 12:00 (T3-5 VAS)
Administered at hourly intervals (from pre-breakfast VAS) for 3 hours
Subjects will complete the same seven-component VAS questionnaire as used pre-breakfast, providing a pre-meal set of ratings.
12:55 (T6 VAS)Subjects will complete a pre-lunch, seven-component VAS questionnaire to measure hunger and other subjective variables.
13:00Subjects will be given an ad libitum lunch. The meal consists of a multiple item buffet (mix of high-fat and low-fat savoury items) and subjects may eat ad lib, and signal when they have finished the meal.
13:30 (T7 VAS)Subjects will complete a post-lunch, seven-component VAS questionnaire to measure hunger and other subjective variables.
Participants also complete a post-lunch palatability VAS.
14:00, 15:00, 16:00, 17:00 (T8-11 VAS)
Administered at hourly intervals (from pre-breakfast VAS) for 4 hours
Subjects will complete the same seven-component VAS questionnaire as used pre-breakfast, providing a post-meal set of ratings.
  • *Timings will be adjusted to facilitate an amended arrival time. All events will remain at the same relative intervals.

  • VAS, Visual Analogue Scale.