Table 2

Biological samples to be collected for storage within the Southampton Research Biorepository

Biological sample	Proposed analysis
Blood	Whole blood sample for genotyping (10 mL EDTA tube), serum (5×10 mL serum tube), plasma (10 mL EDTA tube), mRNA (PAXgene) (total ~80 mL). In addition to these blood samples taken universally, subgroups of participants may be invited to donate a further sample of blood (up to 100 mL) for isolation of immune cells for ex vivo experimental study and for gene array. Serial sampling may be required (up to once a day); however, no more than 210 mL will be taken within a 2-week period.
Urine	Up to 50 mL of urine may be taken for characterisation of biomarkers of disease.
Saliva	Up to five samples within a 14-day period
Nasal samples	Nasal brushing (for microbial carriage analysis and culture of nasal epithelial cells) and nasosorption for mucosal sampling (for inflammatory indices) may be performed as part of the study. Serial sampling may be required, but no more than one nasal brushing, and six nasosorption samples will be taken in a 2-week period.
Exhaled breath samples	Exhaled breath condensate may be collected for assessment of volatile compounds which may influence disease progression. Serial sampling may be required, but no more than two samples will be taken within a 2-week period.
Sputum samples	Excess sputum samples collected as part of routine clinical practice may be processed and analysed for presence of microbes, as well as inflammatory cell infiltrate, gene expression and other immune/inflammatory indices.
Bronchoscopy samples	Non-directed bronchial wash via a suction catheter may be performed in intubated and ventilated patients only. Samples will be processed for microbial analysis, cell culture and molecular biology. Serial sampling may be required, but no more than one sample per day will be taken.