Table 3

Serological detection method

IndexSerological detectionDetecting instrument
OSTElectrochemiluminescence immunoassayAutomatic biochemical analyzer (cobas 8000 e602, Roche)
β-CTXElectrochemiluminescence immunoassayAutomatic biochemical analyzer (cobas 8000 e602, Roche)
PINPElectrochemiluminescence immunoassayAutomatic biochemical analyzer (cobas 8000 e602, Roche)
PTHElectrochemiluminescence immunoassayAutomatic biochemical analyzer (cobas 8000 e602, Roche)
ALPContinuous monitoring techniqueAutomatic biochemical analyzer (modular P800, Roche)
FGF23ELISAMicro plate reader (MK3, Thermo, Waltham, Massachusetts, USA)
Vitamin B6High efficiency liquid chromatographyUltra-performance liquid chromatograph (Agilent 1290 Infinity, Santa Clara, California, USA)
25(OH)DHigh performance liquid chromatography–tandem mass spectrometryLiquid chromatography tandem mass spectrometry (API4000, AB SCIX, Framingham, Massachusetts, USA)
CaO-cresolphtalein-complexone method and phosphomolybdate ultraviolet colorimetryAutomatic biochemical analyser (modular P800, Roche)
PO-cresolphtalein-complexone method and phosphomolybdate ultraviolet colorimetryAutomatic biochemical analyzer (modular P800, Roche)
DBPELISAMicro plate reader (MK3, Thermo, Waltham, Massachusetts, USA)
  • ALP, alkaline phosphatase; Ca, serum calcium; β-CTX, β-C-terminal telopeptide of type I collagen; DBP, vitamin D binding protein; FGF23, fibroblast growth factor 23; 25(OH)D, 25-hydroxyvitamin D3 and D2; OST, osteocalcin; P, serum phosphorus; PINP, propeptide of type I collagen; PTH, parathormone.