Primers for the detection of Leptospira DNA in clinical samples
| Primer/probe sequence | Annealing temperature | Complementary target species |
| Forward: 5’-AAG CAT TAC CGC TTG TGG TG-3’ Reverse: 5’-GAA CTC CCA TTT CAG CGA TT-3’ | 60°C | lipL32 gene of pathogenic Leptospira spp. |
| Forward: 5’-TAA AGG CTC ACC AAG GCG AC-3’ Reverse: 5’-TTA GCC GGT GCT TTA GGC AG-3’ | 60°C | 16 s gene of pathogenic Leptospira spp. |