Table 3

Primer pairs for detection of species

Primer/Probe sequenceAnnealing temperatureComplementary target species
Forward: 5’-CTT GAG CCT GCG CGT TAY-3’
Reverse: 5’-CCG ATA ATT CCA GCG AAG ATC-3’
63°CsecY gene of L. interrogans
Forward: 5’-GAT TCG GGT TAC AAT TAG ACC-3’
Reverse: 5’-TTG ATC TAA CCG GAC CAT AGT-3’
65°CompL1 gene of L. borgpetersenii
Forward: 5’-CTG GCT TAA TCA ATG CTT CTG-3’
Reverse: 5’-CTC TTT CGG TGA TCT GTT CC-3’
60°CsecY gene of
L. kirschneri
Forward: 5’-TCA GGG TGT AAG AAA GGT TC-3’
Forward: 5’-CAA AAT TAA AGA AGC AAA GA-3’
63°CsecY gene of L. noguchii