Table 2

Data and sample collection in the Uppsala–Stockholm Assisted Reproductive Techniques study

PhaseMeasurements
Baseline, collected prior to IVF cycle start

  • Questionnaire: self-reported sociodemographic, health, lifestyle and behaviour in the past 3 months or 1 year (listed in table 2)

  • Blood samples collected: plasma, serum, extracted DNA stored at –80°C.

  • Saliva samples collected at 7 am and 9 pm on the same day, cortisol extracted and measured

At oocyte aspiration

  • Questionnaire, follow-up: a shorter version of the baseline questionnaire to assess if the participant changed their lifestyle factors after cycle start

At delivery

  • Maternal blood sample (PAX gene, EDTA)

  • Cord blood (PAX gene, EDTA)

  • Placenta tissue and amniotic fluid, stored at −80°C

Repeat IVF treatments

  • Questionnaire, follow-up: a shorter version of the baseline questionnaire to assess if the participant changed their lifestyle factors between IVF cycles

  • Blood samples collected: plasma and serum stored at –80°C.

  • Saliva samples collected at 7 am and 9 pm on the same day

  • EDTA, ethylenediaminetetraacetic acid; IVF, in vitro fertilisation.