Table 1

Inclusion and exclusion criteria for donors in the Gut Bugs Trial

Inclusion	Aged 18–28 years
	Body mass index >18.5 kg/m² and <30.0 kg/m^2.
	Total body fat ≤29% for females and ≤19% for males.
	Regular exercise (moderate to vigorous physical activity for at least 3.5 hours per week).
	Regular bowel habit (at least once every 2 days).
	Intake of ≥4 portions of fruit and/or vegetables per day.
Exclusion	Any transmissible viral or bacterial pathogens, or intestinal parasites.
	Multidrug-resistant organisms (eg, vancomycin-resistant enterococci, extended-spectrum beta-lactamase-producing Enterobacteriaceae and carbapenem-resistant Enterobacteriaceae).
	Gastrointestinal disease (including symptoms of irritable bowel syndrome, inflammatory bowel disease or coeliac disease).
	Atopic diseases requiring regular prophylaxis or treatment.
	Current or past history of malignancy.
	Impaired fasting glucose or impaired glucose tolerance.
	Type 1 diabetes, type 2 diabetes or monogenic diabetes.
	Known dyslipidaemia, hypertension or metabolic syndrome.
	Regular use of medications known to influence metabolism or the gut microbiome.
	Use of oral antibiotics in the past 3 months.
	Regular ‘binge drinking’, that is, consumption of 5 or more standard drinks of alcohol per session, at least once a week.
	Any use of recreational drugs or tobacco.
	Current or past pregnancy.
	Overseas travel in previous 6 months, except for visits to Australia, UK, USA, Canada, Northern Europe, France and Germany.
	UK residence in 1980–1996 (due to risk of variant Creutzfeldt-Jakob disease).

Exclusion criteria adapted from Youngster et al, Hirsch et al, van Nood et al and Bakken et al 17–19 34