Omics | Sample | Platform | Features | Subcohort (n=1300)* | Second period child panel study |
Proteomics | Plasma | Luminex kits: cytokines 30-plex, apoliprotein 5-plex and adipokine 15-plex | 36 | 1170 | 154 |
Methylation | Buffy coat | 450K, Illumina | 386 518 | 1173 | 153 |
Transcriptomics | Whole blood | HTA V.2.0, Affymetrix | 35 841 | 1010 | 127 |
MicroRNA (miRNA) | Whole blood | SurePrint Human miRNA rel 21, Agilent | 330 | 941 | 123 |
Urinary metabolomics | Urine | 1H NMR spectroscopy | 44 | 1198 | 153 |
Serum metabolomics | Serum | AbsoluteIDQ p180 kit, Biocrates | 177 | 1198 | 154 |
Telomere length Mitochondrial DNA content | Buffy coat | Quantitative real-time PCR | 2 | 1166 | 153 |
↵*One child’s parents in the subcohort did not give genetic consent, therefore this child was excluded from all omics analyses.
HELIX, Human Early Life Exposome.