Quantity | Special handling | Techniques of analysis | |
---|---|---|---|
Insulin and glucose | 2 mL | Potassium oxalate/sodium fluoride tube (Grey Top) | ELISA and biochemistry analyzer |
HBA1C | SST | Standard procedure | |
Testosterone every 4 weeks | 4 mL | SST | Liquid chromatography with isotope dilution mass spectrometry detection |
Albumin* | SST | Standard procedure | |
SHBG* | SST | Standard procedure | |
IGF-1, IGFBP-1 and 3 | 4 mL | SST | ELISA |
Inflammatory biomarkers (CRP, IL-6, TNFα) | 4 mL | SST | ELISA |
Free fatty acids | 2 mL | Potassium oxalate/sodium fluoride tube (Grey Top) | Enzymatic colorimetric quantification |
Triglycerides, total cholesterol, HDL, LDL | 4 mL | SST | Enzymatic colorimetric quantification |
*Only at baseline 2 and postintervention 1 to calculate free testosterone.
CRP, C reactive protein; HBA1C, haemoglobin A1c; SHBG, sex hormone binding globulin; IGF-1, insulin-like growth factor 1; IGF-BP, insulin-like growth factor binding protein; IL-6, interleukin 6; SST, serum separator tube; TNFα, tumour-necrosis factor α.