Multilocus sequence typing scheme for Trichomonas vaginalis
| MLST locus Accession number | Primer | Sequences (5′- 3′) | Gene size (bp) | 5′ position in gene* | Sequence length† | Total number of alleles (previously reported)‡ |
|---|---|---|---|---|---|---|
| Tryptophanase (P1) XM_001330582.1 | Outer Forward | GAGATGTTCAAGTATGCCGATG | 1451 | 739 | 351 | 6 (6) |
| Outer Reverse | CAAGCTGGACTGTGAAGTGG | 1356 | ||||
| Inner Forward | CGTCAACATCGGTGGCTTCA | 792 | ||||
| Inner Reverse | GCGACAGCGACGACATTCAT | 1261 | ||||
| Glutaminase (p3) XM_001295355.1 | Outer Forward | AAACGCTGGTGCCATTACAAC | 886 | 301 | 285 | 13 (5) |
| Outer Reverse | AAGGTTCTTGCCACGGATTG | 822 | ||||
| Inner Forward | TCGTTAATAGTGGGTAAGGACG | 326 | ||||
| Inner Reverse | CCAAGTATAGCTCCGCTGAC | 740 | ||||
| Family T2 aparaginase-like threonine peptidase (P6) XM_001323020.1 | Outer Forward | AAGGCTGTTGAGCGTGGTGC | 990 | 94 | 318 | 8 (6) |
| Outer Reverse | GGTGTTCCGCCTGTAGAAGTGC | 560 | ||||
| Inner Forward | GAACAGGAGCACCAGCAGAA | 125 | ||||
| Inner Reverse | TCTCTAGCAACGCAGCCAAC | 517 | ||||
| Alanyl tRNA synthetase (P8) XM_001315470.1 | Outer Forward | AAGGAGGCAGAAGACAAGTGG | 3075 | 2167 | 430 | 20 (18) |
| Outer Reverse | TGGAGCATACTTAGGAATGTTAGC | 2833 | ||||
| Inner Forward | TCTGTCCAGGATGGTGTCTT | 2332 | ||||
| Inner Reverse | ACGCCTTCCTCCTTCATCTT | 2806 | ||||
| DNA mismatch repair protein (P13) XM_001301638.1 | Outer Forward | GGACTATCCTACGATGCTATTGAG | 1758 | 1132 | 447 | 14 (12) |
| Outer Reverse | GTGACCAAATTTCGCACCAAG | 1679 | ||||
| Inner Forward | TCAAGGATATGGAAATCATCG | 1166 | ||||
| Inner Reverse | GTCTTCCGTGCGGACAATTC | 1656 | ||||
| Serine hydroxymethyltransferase (P14) XM_001322558.1 | Outer Forward | CAGGTGATACATTTATGGGATTG | 1356 | 383 | 339 | 2 (2) |
| Outer Reverse | GGCTATCTGTTCCTCCACTGAC | 985 | ||||
| Inner Forward | GCTGAGTGACGGTGGAACATT | 408 | ||||
| Inner Reverse | GAAGATGAGGTCCTCCTTGA | 837 | ||||
| Mannose 6-phosphate isomerase (P16) XM_001325698.1 | Outer Forward | TTACGGTTTTCGTCCATTTG | 1128 | 372 | 372 | 5 (6) |
| Outer Reverse | GACAACAACACCTCTTCCTGC | 1030 | ||||
| Inner Forward | AGCCAGTTGGCTTCTGAGTT | 538 | ||||
| Inner Reverse | AACAATTCCGCAAGCTGGAG | 977 |
Italics: previously published, Cornelius et al.13
*As the exact chromosomal location of each MLST gene is unknown, positions are given in base pairs of each MLST gene relative to the reference sequence G3 (GenBank accession no: NW_001517113.1).
†Sequences were trimmed to the previously defined length, specified per locus.13
‡The total number of alleles found for each locus in this study. Total numbers of alleles found previously are given in brackets.
bp, base pairs; MLST, multilocus sequence typing.