RT Journal Article SR Electronic T1 Unusually low prevalence of Mycoplasma genitalium in urine samples from infertile men and healthy controls: a prevalence study JF BMJ Open JO BMJ Open FD British Medical Journal Publishing Group SP e005372 DO 10.1136/bmjopen-2014-005372 VO 4 IS 8 A1 Vanda Plecko A1 Lidija Zele-Starcevic A1 Vesna Tripkovic A1 Mihael Skerlev A1 Suzana Ljubojevic A1 Sanja Plesko A1 Ivana Marekovic A1 Jorgen Skov Jensen YR 2014 UL http://bmjopen.bmj.com/content/4/8/e005372.abstract AB Objective To detect Mycoplasma genitalium in urine samples of infertile men and men without any signs of infection in order to investigate whether M. genitalium and other genital mycoplasmas (Mycoplasma hominis and Ureaplasma spp) are found more often in urine samples of infertile men than in asymptomatic controls and to determine resistance to macrolides. Methods The study included first void urine samples taken from 145 infertile men and 49 men with no symptoms of urethritis. M. genitalium, Chlamydia trachomatis and Neisseria gonorrhoeae were detected by commercial PCR. Trichomonas vaginalis was detected by microscopy and culture. M. hominis and Ureaplasma spp were detected by culture. M. genitalium was detected by in-house conventional and real-time PCR. Results Two M. genitalium positive samples were found among samples obtained from infertile men. All asymptomatic men were M. genitalium negative. Macrolide resistance was not found in either of the two positive samples. Conclusions In comparison with reported data, an unusually low prevalence of M. genitalium was found in infertile men. The reasons for this unexpected result are not known; possibly, local demographic and social characteristics of the population influenced the result. Further studies to investigate M. genitalium in infertile and other groups of patients are needed.