TY - JOUR T1 - Seroprevalence of <em>Entamoeba histolytica</em> at a voluntary counselling and testing centre in Tokyo: a cross-sectional study JF - BMJ Open JO - BMJ Open DO - 10.1136/bmjopen-2019-031605 VL - 10 IS - 2 SP - e031605 AU - Yasuaki Yanagawa AU - Mami Nagashima AU - Hiroyuki Gatanaga AU - Yoshimi Kikuchi AU - Shinichi Oka AU - Keiko Yokoyama AU - Takayuki Shinkai AU - Kenji Sadamasu AU - Koji Watanabe Y1 - 2020/02/01 UR - http://bmjopen.bmj.com/content/10/2/e031605.abstract N2 - Background Amebiasis, which is caused by Entamoeba histolytica, is a re-emerging public health issue owing to sexually transmitted infection (STI) in Japan. However, epidemiological data are quite limited.Methods To reveal the relative prevalence of sexually transmitted E. histolytica infection to other STIs, we conducted a cross-sectional study at a voluntary counselling and testing (VCT) centre in Tokyo. Seroprevalence of E. histolytica was assessed according to positivity with an ELISA for E. histolytica-specific IgG in serum samples collected from anonymous VCT clients.Results Among 2083 samples, seropositive rate for E. histolytica was 2.64%, which was higher than that for HIV-1 (0.34%, p&lt;0.001) and comparable to that for syphilis (rapid plasma reagin (RPR) 2.11%, p=0.31). Positivity for Chlamydia trachomatis in urine by transcription-mediated amplification (TMA) was 4.59%. Seropositivity for E. histolytica was high among RPR/Treponema pallidum hemagglutination (TPHA)-positive individuals and it was not different between clients with and without other STIs. Both seropositivity of E. histolytica and RPR were high among male clients. The seropositive rate for anti-E. histolytica antibody was positively correlated with age. TMA positivity for urine C. trachomatis was high among female clients and negatively correlated with age. Regression analysis identified that male sex, older age and TPHA-positive results are independent risk factors of E. histolytica seropositivity.Conclusions Seroprevalence of E. histolytica was 7.9 times higher than that of HIV-1 at a VCT centre in Tokyo, with a tendency to be higher among people at risk for syphilis infection. There is a need for education and specific interventions against this parasite, as a potentially re-emerging pathogen. ER -