Objective The goal of this study is to investigate the associations of apolipoprotein A5 (APOA5) polymorphisms with coronary artery disease (CAD) in a Chinese population.
Method This case–control study included 710 subjects (355 patients with CAD and 355 controls) who were recruited from a cross-sectional study. Three single nucleotide polymorphisms (SNPs) rs662799 (−1131T>C), rs651821 (−3A>G) and rs2075291 (G185C) in APOA5 were selected and genotyped using the matrix-assisted laser desorption ioniasation time of flight mass spectrometry technology. The χ2 test and haplotype analysis were performed to analyse the associations between APOA5 SNPs and CAD using the SPSS V.22.0 software package and the online SNPStats program.
Results APOA5 SNPs rs662799 and rs651821 exhibited significant differences in genotype and allele distributions between patients with CAD and control subjects. The SNP rs662799 was significantly correlated with an increased risk of CAD when a dominant model was considered. The SNP rs651821 was significantly correlated with an increased risk of CAD when a codominant model was considered. Moreover, the variant C alleles of rs662799 and the variant G alleles of the rs651821 polymorphism were significantly correlated with increased plasma triglyceride (TG) levels in the CAD group (all p<0.05). Additionally, a mediating effect of TG on the associations between the APOA5 rs662799 and rs651821 polymorphisms and CAD was observed.
Conclusion Based on these data, variants of the APOA5 gene are associated with CAD susceptibility and may modulate plasma TG levels among a Chinese population.
- coronary artery disease
- single nucleotide polymorphisms
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Contributors YY, Y-HW, YZ, LZ, CK and YY: designed and performed the study. YY, Yan-HuaWu, YZ and YS: analysed the data. YY: drafted the manuscript. YY, WB, YL and YZ: participated in revising draft of the manuscript. All authors approved the final version of the manuscript.
Funding This study was funded by The scientific research foundation of the Jilin Provincial Health Department, China (grant#2011Z116).
Competing interests None declared.
Patient consent Obtained.
Ethics approval All participants in this study provided written informed consent form and our study was approved by the ethics committee of the School of Public Health, Jilin University (reference number: 2012-R-011).
Provenance and peer review Not commissioned; externally peer reviewed.
Data sharing statement No additional data are available.
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