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Pregnancy diet and offspring asthma risk over a 10-year period: the Lifeways Cross Generation Cohort Study, Ireland
  1. Karien Viljoen1,
  2. Ricardo Segurado1,2,
  3. John O’Brien1,
  4. Celine Murrin1,
  5. John Mehegan1,
  6. Cecily C Kelleher1
  7. DMed on behalf of the Lifeways Cross Generation Cohort Study Steering Group
    1. 1 School of Public Health, Physiotherapy and Sport Science, University College Dublin, Dublin, Ireland
    2. 2 Centre for Support and Training in Analysis and Research, University College Dublin, Dublin, Ireland
    1. Correspondence to Dr Karien Viljoen; karien.viljoen{at}ucdconnect.ie

    Abstract

    Objective The association of maternal pregnancy diet with offspring asthma risk have been reported. However, literature on longitudinal patterns of asthma risk relative to intrauterine nutrient exposure is limited. We aimed to establish whether vegetable, oily fish and vitamin D intake during pregnancy are associated with childhood asthma risk over a 10-year period in the Irish Republic.

    Design Mother–child pairs (n=897) from the Lifeways prospective birth cohort, with data on nutrient intake during pregnancy and asthma status, respectively, were eligible for inclusion in the analysis. Data on socioeconomic and morbidity indicators over 10 years of follow-up on mothers and the index child were collected through self-administered questionnaires. Asthma status as diagnosed by the general practitioner at any time point over 10 years was related to maternal vegetable, oily fish and vitamin D intake during pregnancy, while adjusting for gestational age, socioeconomic status, smoking at delivery, breast feeding, season of birth and supplement use. Data were modelled with a marginal model on correlated observations over time within individuals.

    Results In the fully adjusted model, asthma was inversely associated with higher daily average intake of oily fish (OR 0.23 per serving/day, 95% CI 0.04 to 1.41) and of vegetables (OR 0.96 per serving/day, 95% CI 0.88 to 1.05), but the confidence limits overlapped 1. A higher daily vitamin D intake was associated with reduced odds of asthma (OR 0.93 per μg/day, 95% CI 0.89 to 0.98).

    Conclusion This analysis suggests higher daily average intake of vitamin D in pregnancy is associated with asthma risk in offspring over the first 10 years of life.

    • asthma
    • childhood
    • DOHaD
    • pregnancy
    • vitamin D

    This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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    Strengths and limitations of this study

    • The prospective design of this study is with an a priori purpose of examining intergenerational transmission of risk.

    • As there is a strong social gradient applied to diet in pregnancy, confounding by sociodemographic factors and lifestyle was a concern. We controlled for a comprehensive range of sociodemographic factors.

    • The marginal model enabled us to take advantage of repeated measurements over time for participants.

    • Limitations were differential follow-up, leading to varied numbers of missing data for certain variables, potentially introducing bias to follow-up data. Countering this, it is notable that the same associations were found separately at the three different time points.

    • The authors recognise that asthma in childhood is a heterogeneous phenotype and that grouping of the three time points could potentially mask different phenotypes; however, our study was not powered to differentiate asthma on phenotype at various follow-up. In addition to this, data on familial atopy and fetal growth parameters were not available; General Practitioner’s criteria to diagnose asthma could be diverse, with potential for variability.

    Introduction

    Asthma is the most common chronic disease of childhood1 2; reports indicate a continuous and consistent increase in worldwide prevalence, especially in westernised societies. Prevalence rates in the UK and Ireland are among the highest in Europe.3 4 According to the Centres for Disease Control and Prevention, between 2001 and 2010, asthma prevalence in children in the USA increased 1.4% each year.5 This increase is most likely multifactorial, with complex interactions of genetic, immunological and environmental factors, leading to the phenotypic expression of disease.6 Recently, multiple studies have attempted to deconstruct this multifactorial relationship, focusing on the change in dietary habits over recent decades.7–13 The progressive trend in early presentation of allergic disease in childhood, with the implication of possible exposure in utero, has placed an emphasis on maternal pregnancy diet as a prominent factor in the development of offspring asthma.14 15 As allergen-specific immune responses are established in fetal life, maternal nutrient intake during pregnancy is pivotal; intake may potentially influence the development of both the innate and acquired immune responses, predisposing to atopy in later life.16

    A growing body of persuasive epidemiological evidence suggests that deficiency of maternal vitamin D intake prenatally has an inverse relationship with atopic disease in childhood.17–22 Observational studies on the association of maternal serum and/or infant cord blood 25-hydroxy-vitamin D3 (25(OH)D3) levels with atopic markers are conflicting. Some demonstrated similar inverse associations23–30; others demonstrated direct31–33 and null34–42 associations. U-shaped associations were suggested by both Rothers et al 43 and Maslova et al.44 Most recently, intervention studies exploring pregnancy vitamin D supplementation and asthma risk at 3 years of age also suggested an inverse association (statistically non-significant). The authors suggested that longer follow-up of children is needed to determine the clinical importance of findings.45 46

    The Lifeways study has previously reported the association between pregnancy intake of oily fish and vegetables and general practitioner (GP) diagnosed asthma in offspring at age 3 years; a higher daily mean intake suggested a significant inverse effect.47 Literature on pregnancy consumption of fish with subsequent atopic risk in offspring is mostly consistent with our findings, indicating an inverse effect.7 48–54 Our current aim was twofold: first is to build on the aforementioned Lifeways findings and test the hypothesis that a higher pregnancy intake of vegetables and oily fish might be inversely associated with asthma risk at any stage over 10 years follow-up; second, as suggested in the literature, to explore the association of pregnancy vitamin D intake and offspring asthma risk within our cohort at any stage over 10 years of follow-up.

    Methods

    Study design and sample selection

    The Lifeways study was established 2001–2003 as a prospective birth cohort in Ireland. The a priori purpose was to examine determinants of health status in children, including diet and lifestyle and to establish patterns and links across generations. Recruitment, data collection and study instruments have previously been discussed in detail.55 In brief, mothers were recruited at first antenatal visit (14–16 weeks gestation) in one of two regional maternity hospitals in the more rural West (Galway) and the more industrialised East (Dublin). Of 1124 mothers recruited, 1082 gave birth resulting in 1096 live mother–child pairs. Analysis was limited to current live mother–child pairs, where data on the proband’s asthma status were available for at least one time point. Babies with congenital anomalies and delivery <34 weeks gestational age56 were excluded from analysis. Due to attrition over time, participants had differential patterns of follow-up through phases; 614 mother–child pairs were included in the year 3 analyses, 511 in year-5 and 432 in year-10 follow-up. The sample for this analysis of 897 mother–child pairs comprised respondents for whom at least one follow-up point of asthma health status was recorded (figure 1). Ethical approval for all phases of the study was granted by the Human Research Ethics Committee, University College Dublin, Ireland. Written informed consent was obtained from all adult participants at each follow-up phase of the study. Parental consent was obtained for child subjects at each follow-up phase; additional assent was obtained from all child subjects at the year-10 follow-up phase.

    Figure 1

    Numbers through analysis. A total of 1096 mothers had a pregnancy which resulted in a live birth at cohort initiation. Mothers with a null FFQ and missing asthma data saw 897 mother–child pairs with asthma data at any time point of follow-up included in the final multivariable analysis. Attrition over time led to 614 mother–child pairs at year 3 follow-up, 511 at year 5 follow-up and 432 at year 10 follow-up.

    Assessment of pregnancy diet

    Data on maternal nutrient intake during pregnancy were captured by a semiquantitative Food Frequency Questionnaire (FFQ) as part of a self-administered questionnaire to the mother at her first antenatal visit. The FFQ was developed from the international version used in the European Prospective Investigation in Cancer studies by the National Nutritional Surveillance Centre and extensively validated for use in an Irish population by the National University of Ireland, Galway.57 The main food groups regularly consumed in the Irish diet were included and consisted of 149 food items.47 58 The questionnaire focused on maternal dietary intake since pregnancy. Intake as a medium serving (detailed in the FFQ for relevant food items) was recorded on a 9-grade scale, with categories subsequently transformed to continuous daily portion averages for all 149 food items. To arrive at distinct food groups, the continuous intake of various food items were summed and reported as total portions per day (see online supplementary table S1). The daily average intake of energy and nutrients, that is, ingested portions/day of food containing vitamin D, was calculated by linking frequency selections from the FFQ with food equivalents in McCance and Widdowson’s nutritional composition database, sixth edition,59 using software developed specifically for the Lifeways database.60 An estimation of the intake of Vitamin D in micrograms per day (μg/day) and energy in kilocalories (kCal/day) were made.

    Supplementary file 1

    Assessment of outcome

    Data on doctor diagnosed asthma in offspring were collected at three time points up to year 10 of follow-up: ages 3, 5 and 9 years old. Various studies have used doctor diagnosed asthma/parental report of doctor diagnosed asthma to ascertain diagnosis.30 45 Questions to ascertain asthma diagnosis were adapted from the validated International Study on Asthma and Allergies in Childhood questionnaire.61 62 Diagnosis from age 3 and 9 follow-ups were reported by the general practitioner (GP), information on GP diagnosed asthma at age 5 was obtained from the mother. For the univariate analysis, asthma as a dichotomous outcome variable (‘yes’ vs ‘no’) at each of the three time points were analysed separately. For the multivariable analysis, the effect of dietary intakes during pregnancy on doctor diagnosed asthma in the child at the three time points over a period of 10 year follow-up was examined controlling for different prevalence at each age but assuming protective factors would have the same effect at each age.63

    Statistical analysis

    Definite doctor diagnosed asthma in offspring at any time point over 10-year follow-up was related to maternal pregnancy intake of oily fish, vegetables, expressed in servings per day and vitamin D expressed in microgram per day.

    Univariate analysis and covariates

    Variables that could potentially confound the diagnosis of asthma were identified from the literature and screened in our cohort for association with an asthma diagnosis at the specific time point using unadjusted binomial logistic regression or the independent samples t-test. Predictors with P<0.1 were identified as being potential confounders and selected for inclusion in the multivariable models and are discussed below.

    Total energy intake

    To control for variance in accuracy of maternal energy reporting, total energy intake (kCal) was adjusted for in all models. Nutrients were adjusted for total energy intake using the residual method.64 65 To account for mothers who potentially over-reported or under-reported energy intake, the ratio of energy intake (EI) to basal metabolic rate in the first trimester of pregnancy (BMRpreg) was calculated to identify extreme outliers.63 66 67 BMRpreg was calculated using the prepregnancy BMI and Schofield equations.67 Mothers were asked to report their prepregnancy weight at consultation during the first antenatal visit.

    Gestational age

    Gestational age in weeks.

    Socioeconomic status

    We used eligibility for the general medical scheme (GMS) as an indicator of socioeconomic status. Ireland has a two-tiered healthcare system, where certain individuals are eligible for different levels of free healthcare dependent on their income and certain medical conditions. GMS is thus a robust means tested indicator.68–70 As maternal and offspring GMS eligibility are strongly correlated, only the maternal predictor was used in the models.63 71

    Smoking

    Smoking status at time of delivery; hospital delivery records.

    Breast feeding

    Data from the self-administered maternal questionnaire, ‘Was your Lifeways child ever breastfed?’

    Season of birth

    Seasons were composed as follows: summer (May, June, July), autumn (August, September, October), winter (November, December, January), spring (February, March, April). This follows the grouping suggested by the Irish Randomised cOntrol trial of LOw (ROLO) glycaemic index diet study with data on maternal serum 25(OH)D levels.72 Summer and autumn were collapsed to form ‘summer’, with spring and winter collapsed to form ‘winter’, making up the final dichotomous variable, ‘summer’ vs ‘winter’.

    Supplement use

    Data on supplement use were generic: ‘Have you taken any vitamins, minerals or food supplements?’ ‘Yes’ or ‘no’ and did not allow for specification on supplement type or content quantification.

    Multivariable analysis

    The associations with the three aforementioned dietary intakes were analysed in multivariable models to assess their independent association with asthma and verify the extent of confounding in a stepwise manner. Vegetables, oily fish and vitamin D intakes were analysed as continuous predictor variables. Multivariable analysis was run using the full sample of 897 mother–child pairs in a marginal model (or Generalised Estimating Equation model) with repeated measures of asthma over time with an unstructured variance–covariance matrix to allow unequal variances and residual correlations. Covariates were entered into the model sequentially as fixed factors based on their univariate association with asthma, up to the cut-off of P=0.1. Time was consistently included as a fixed factor. Predictor variables were regarded as significant if the 95% CIs for the ORs did not include 1. SPSS V.20 was used to conduct univariate analysis; multivariable analysis was run with SAS V.9.3.

    Sensitivity analysis

    Considering that non-response (attrition or sparse point-wise) bias may be introduced by residual socioeconomic or educational factors which correlate with diet and with asthma diagnosis, we applied a sensitivity analysis to explore how robust the observed associations were.73 We assume no or a smaller effect in the full sample and that non-response arises in a subgroup with elevated asthma prevalence and decreased quality of dietary intake and noted under which conditions false associations exist in the responders.

    Results

    Study subjects’ characteristics and asthma prevalence

    In the final sample, 66.9% of the mother–child pairs were resident in the Dublin area and 33.1% in the Galway area, proportionate to recruitment patterns. Mothers with a third-level education were marginally higher (51.9%) than those with none/primary/secondary school education (48.1%); 19.4% of mothers were smokers at the time of giving birth. The mean (SD) age of mothers at time of giving birth was 30.2 (5.9) years and the mean (SD) prepregnancy body mass index of mothers were 23.7 (4.0). The offspring sex distribution was about equal; 48.8% were male and 51.2% were female. The mean (SD) birth weight was 3515.3 (568.6) g, with a mean (SD) gestational age of 39.9 (1.9) weeks. Just under half (45.8%) of probands were the first-born and 86.20% were delivered vaginally. Doctor diagnosed asthma in offspring at the three phases of follow-up, respectively, increased from 10.90% at 3 years to 14.33% at 5 years and 23.10% at 10 years. In general, the literature suggests a downward trend in prevalence with increasing age. There are, however, cohorts that noted an increase of prevalence with age.74–76 Maternal socioeconomic and biological characteristics relative to food group intake are presented in table 1. Maternal and child characteristics as related to asthma diagnosis are further presented in supplemental material (online supplementary tables S2 and S3).

    Table 1

    Maternal food group intake during pregnancy in relation to socioeconomic factors (n=897)

    Univariate associations of background variables with childhood asthma from the cross-sectional data for the three follow-up phases, respectively, have previously been discussed in detail.47 62 63

    Within-time representativeness of responders versus non-responders that contributed to the final sample is presented in table 2.

    Table 2

    Within-time representativeness of final sample (n=897)

    Pregnancy dietary intake

    Reported intake of vegetables and oily fish were directly and significantly associated with the maternal social gradient (table 1). Mean (SD) daily intake of oily fish and vegetables were 0.06 (0.12) and 2.93 (2.04) portions per day, respectively. The Food Safety Authority of Ireland (FSAI) recommends that pregnant women should have one portion of oily fish weekly (ie, 0.1 portions/day) and six to seven portions of vegetables daily.77 The mean intake of Lifeways mothers did not reach this recommendation. Data captured by the FFQ detailed intake as a medium portion (90–100 g of fish; a piece of fish about the size of a woman’s palm).

    Vitamin D intake during pregnancy between those mothers with consistent follow-up and those with attrition did not differ significantly. A social gradient for pregnancy vitamin D intake in the final sample was not observed. The distribution of mean vitamin D intake was investigated according to the EI:BMRpreg ratio; no marked difference in the distribution of vitamin D was observed.63 Energy adjusted mean (SD) intake of vitamin D in mothers was 4.3 (4.1) μg/day. The FSAI recommends a daily allowance of 10 µg/day vitamin D for Irish pregnant women78 (see online supplementary tables S4 and S5). At baseline, 327 (36.9%) mothers from the final sample reported using supplements.

    Pregnancy dietary associations with offspring asthma

    Vegetable intake was negatively associated with offspring asthma, although not significantly so in the fully adjusted model (OR 0.96 per serving/day, 95% CI 0.88 to 1.05) (model 8) (table 3).

    Table 3

    Marginal model: asthma at any time point (vegetable intake as main exposure of interest)

    Oily fish intake was inversely associated with offspring asthma in the first two models, with an increase in daily average serving of oily fish demonstrating lower odds at any time point over the 10-year follow-up period (model 2 OR 0.17 per serving/day, 95% CI 0.03 to 0.87) (table 4). Change in the ORs across models suggested that gestational age may have been a confounder (model 2 vs model 3), and all models up to the fully adjusted (OR 0.23 per serving/day, 95% CI 0.04 to 1.41) were not significant, despite a substantial magnitude of the OR.

    Table 4

    Marginal model: asthma at any time point (oily fish intake as main exposure of interest)

    All models suggested vitamin D had a significant inverse association, including the fully adjusted analysis (model 9 OR 0.93 μg/day, 95% CI 0.89 to 0.98) with offspring asthma at any time-point of follow-up (table 5). Adjustment for breast feeding in particular increased the OR for vitamin D (model 5 vs model 6) in part due to the loss of 85 women with no data on breastfeeding habits.

    Table 5

    Marginal model: asthma at any time point (vitamin D intake as main exposure of interest)

    Results of a sensitivity analysis on the Vitamin D association indicated that if the true association was null, a false association of this magnitude could be generated by high missing data in a subgroup with higher asthma prevalence (by approximately 5%) and lower vitamin D intake (by 10% at the median), but only if the non-responders had an association in the opposite direction (high vitamin D associated with increased asthma risk). An inflation of a true but small effect could also occur in scenarios where non-responders have lower vitamin D intake, similar or lower asthma rates and a weaker association between intake and asthma than responders. The hypothetical scenarios consistent with our expectations of missing data patterns suggest our observed OR of 0.93 may be attenuated relative to the true effect.

    Discussion

    Birth cohort studies with follow-up of 6 years or more on the association of maternal pregnancy diet and childhood asthma are limited.79 This analysis suggests that higher vitamin D intake during pregnancy is associated with reduced likelihood of asthma in offspring at any point during 10 years follow-up. An interesting inverse association with oily fish consumption was also demonstrated initially but with significance lost early in model progression. Vegetable intake, however, showed a very small inverse association not statistically distinguishable from zero at a 5% level. Literature on the association with vegetable intake is scarce and inconsistent, with both an increased risk80 and no effect reported.81 Literature on the association of oily fish intake in pregnancy with offspring atopic disease is consistent with findings from the current analysis.49 51 80 81 Analyses from all of these studies were limited to children in the age range 2–6 years; to our knowledge there are no studies with findings extending up to 10 years of age follow-up. A systematic review82 concluded that there is little evidence to recommend supplementation/modification of diet to include fish oil for children or adults with established asthma. They found no evidence of improvement or increased risk relative to fish oil and established asthma. This is likely evident that the window period for fish oil to have an effect on immune regulation is most relevant in fetal life, with limited potential for effect once immune responses are established.81 The proinflammatory mediators leukotriene B4 (LTB4) and tumour necrosis factor alpha increase airway inflammation and hyper-responsiveness.83 84 A protective effect of oily fish could possibly be mediated via eicosapentaenoic acid and docosahexaenoic acid. Competitive binding with cyclooxygenase and lipoxygenase reduce LTB4 and prostaglandin E2 production.49 85–87

    Our findings suggest a significant inverse association of pregnancy vitamin D consumption with childhood asthma, which in sensitivity analyses appears robust to bias arising from a reasonable range of missing data patterns. Results are consistent with the main body of literature on the association of pregnancy vitamin D intake and offspring atopic disease.17–22 Miyake et al 21 reported that increased vitamin D consumption in pregnancy was associated with less wheeze and eczema in offspring aged 16–24 months. A follow-up paper from the same cohort reported higher intake of maternal vitamin D with an increased risk of eczema in offspring, now 23–29 months old.88 More recently, a significant increase in the risk of asthma in 20–25 years old offspring of mothers who had high concentrations of serum 25(OH)D antenatally was reported.89 We speculate that the long-term effect of prenatal vitamin D exposure differs from the initial, mostly protective, effect. Findings from vitamin D status studies are less consistent and at times not in agreement with intake studies. Lack of concordance in findings may be an indication that intake data are a surrogate for the intake of other important nutrients.15 The possible mechanism of action in the relationship of vitamin D and asthma could relate to T helper 2 cell (Th2) differentiation. Literature is conflicting; one immunological study reported that vitamin D supplementation (in vitro) promoted Th2 cell differentiation.90 It has been proposed that the increased vitamin D supplementation in European and high latitude countries to prevent rickets led to the current high prevalence of asthma.9 91 Contrary to this, the proposal is that vitamin D inhibits Th2 differentiation, thus having a protective effect.92 Vitamin D intervention studies are under way; results from a randomised controlled trial in the UK failed to find any association between prenatal vitamin D supplementation and wheezing in offspring 3 years of age.93 Litonjua et al 45 found a lower incidence (non-significant) of recurrent wheeze and asthma in 3-year-old offspring of mothers who received higher vitamin D supplementation in pregnancy. An Aberdeen cohort demonstrated an in vivo anti-inflammatory effect of maternal serum 25(OH)D3 on interleukin 10 secretion from the airway epithelial cells of cultured neonatal nasal samples.94 Dick et al 95 suggested that a specific exposure might act in a different manner in different population groups and demographic areas, as the genetic and epigenetic factors are likely to be differential. Inconsistencies in findings from the association between pregnancy nutrition and offspring asthma may furthermore relate to interactions between specific nutrients or additional interactions with the human microbiome and environment.96 Strengths of this study and analysis were the prospective design with an a priori purpose of examining intergenerational transmission of risk. As there is a strong social gradient applied to diet in pregnancy,97 confounding by sociodemographic factors and lifestyle was a concern; we controlled for a comprehensive range of sociodemographic factors. The marginal model enabled us to control for repeated measurements over time for participants. Limitations were differential follow-up, leading to varied numbers of missing data for certain variables, potentially introducing bias to follow-up data. Although a sensitivity analysis was encouraging, it remains possible that unknown, unobserved factors driving non-response have generated bias in an unexpected direction, and there is a dearth of literature to guide exploration of such effects. The fact that the associations found in this analysis were between maternal food intake and doctor diagnosed asthma raised the possibility of ascertainment bias; more health conscious mothers were more likely to follow good pregnancy diets and were more likely to take their ill children to the doctor to receive a formal diagnosis. However, we propose that an ascertainment bias would be more likely to demonstrate the opposite finding, that is, that an increase in healthy eating was adversely associated with doctor-diagnosed asthma.

    Countering this, it is notable that the same associations were found separately at the three different time points.47 63 A more comprehensive adjustment (for maternal age, education and region of residence) also did not substantially alter the conclusions and estimates and we feel we have adjusted as thoroughly as possible for potential socioeconomic factors and their influence on bias.

    The authors recognise that asthma in childhood is a heterogeneous phenotype and that grouping of the three time points could potentially mask different phenotypes98 99; however, our study was not powered to differentiate asthma on phenotype at various follow-up. Data on familial atopy or asthma were not available; it is possible that the suggested relationships between maternal diet and offspring asthma have been confounded by the presence or absence of familial predisposition. Data on fetal growth parameters were not available; the effect of fetal growth on childhood asthma could not be controlled for.100 The calculation of vitamin D (μg/day) and energy (kCal/day) intake was a surrogated calculation obtained from an estimated daily intake (portion/day) of food groups/nutrients.59 As no data on serum 25(OH)D3were collected, it was not possible to correlate vitamin D intake with that of serum levels.

    Conclusion

    This analysis suggests that higher intakes of oily fish and vitamin D in the maternal pregnancy diet are inversely associated with childhood asthma. This is consistent with the developmental origins of health and disease hypothesis, suggesting that certain exposures of the fetus in utero can affect the development of allergic diseases in childhood by modulating immune response.101 As there is no anticipated cure for asthma in the near future, adjustment of the environment as early as possible, for example, in utero and in infancy, might provide the best way to achieve a reduction in the asthma burden.95 102

    Acknowledgments

    The participation of families in the various sweeps of the study is highly appreciated. We are grateful to the reviewers of the manuscript for their assistance in refining the analysis and reporting, in particular Professor Charles E McCulloch (UCSF).

    References

    1. 1.
    2. 2.
    3. 3.
    4. 4.
    5. 5.
    6. 6.
    7. 7.
    8. 8.
    9. 9.
    10. 10.
    11. 11.
    12. 12.
    13. 13.
    14. 14.
    15. 15.
    16. 16.
    17. 17.
    18. 18.
    19. 19.
    20. 20.
    21. 21.
    22. 22.
    23. 23.
    24. 24.
    25. 25.
    26. 26.
    27. 27.
    28. 28.
    29. 29.
    30. 30.
    31. 31.
    32. 32.
    33. 33.
    34. 34.
    35. 35.
    36. 36.
    37. 37.
    38. 38.
    39. 39.
    40. 40.
    41. 41.
    42. 42.
    43. 43.
    44. 44.
    45. 45.
    46. 46.
    47. 47.
    48. 48.
    49. 49.
    50. 50.
    51. 51.
    52. 52.
    53. 53.
    54. 54.
    55. 55.
    56. 56.
    57. 57.
    58. 58.
    59. 59.
    60. 60.
    61. 61.
    62. 62.
    63. 63.
    64. 64.
    65. 65.
    66. 66.
    67. 67.
    68. 68.
    69. 69.
    70. 70.
    71. 71.
    72. 72.
    73. 73.
    74. 74.
    75. 75.
    76. 76.
    77. 77.
    78. 78.
    79. 79.
    80. 80.
    81. 81.
    82. 82.
    83. 83.
    84. 84.
    85. 85.
    86. 86.
    87. 87.
    88. 88.
    89. 89.
    90. 90.
    91. 91.
    92. 92.
    93. 93.
    94. 94.
    95. 95.
    96. 96.
    97. 97.
    98. 98.
    99. 99.
    100. 100.
    101. 101.
    102. 102.
    View Abstract

    Footnotes

    • Contributors The Principal Investigator of the Lifeways Study, CCK, conceptualised the design of the study and supervised the overall project, including data analysis and interpretation. KV drafted the manuscript and undertook data collection, analyses and interpretation as part of her PhD. CM assisted with data collection, analyses and interpretation. RS provided statistical input relating to analyses and interpretation of data. JO’B and JM assisted with data management and data interpretation. All contributors reviewed and revised the manuscript and approved the final version as submitted.

    • Funding All stages of this study were funded by the Health Research Board of Ireland.

    • Competing interests None declared.

    • Patient consent Obtained.

    • Ethics approval Human Research Ethics Committee, University College Dublin, Ireland.

    • Provenance and peer review Not commissioned; externally peer reviewed.

    • Data sharing statement Additional unpublished data from the Lifeways study relating to cardiovascular risk/mortality and healthcare utilisation are available. Data can be accessed by collaborators or peers via written request to the principal investigator, CCK, (cecily.kelleher@ucd.ie) and the study data manager, JM (john.mehegan@ucd.ie).

    • Collaborators Lifeways Cross Generation Cohort Study Steering Committee: Professor Gerard Bury, Professor Leslie Daly,Professor Sean Daly, Dr Orla Doyle, Dr Una B Fallon, Dr Frances B Hannon, Dr Howard Johnson, Dr Lucy J Jessop, Professor CCK, Professor Gerard Loftus, Professor John J Morrison, Professor Andrew W Murphy, Dr Celine Murrin,Professor Helen Roche, Dr Mary Rose Sweeney, Professor Richard Tremblay.

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