Objectives The incidence of cervical cancer is up to 20-fold higher among First Nations women in Canada than the general population, probably due to lower participation in screening. Offering human papillomavirus (HPV) self-sampling in place of Papanicolaou (Pap) testing may eventually increase screening participation and reduce cervical cancer rates in this population.
Design A community-randomised controlled screening trial.
Setting First Nations communities in Northwest Ontario, Canada.
Participants Women aged between 25 and 69, living in Robinson Superior Treaty First Nations. The community was the unit of randomisation.
Interventions Women were asked to complete a questionnaire and have screening by HPV self-sampling (arm A) or Pap testing (arm B).
Primary outcome measures The number of women who participated in cervical screening.
Randomisation Community clusters were randomised to include approximately equivalent numbers of women in each arm.
Results 6 communities were randomised to arm A and 5 to arm B. One community withdrew, leaving 5 communities in each group (834 eligible women). Participation was <25%. Using clustered intention-to-treat (ITT) analysis, initial and cumulative averaged uptakes in arm A were 1.4-fold (20% vs 14.3%, p=0.628) and 1.3-fold (20.6% vs 16%, p=0.694) higher compared to arm B, respectively. Corresponding per protocol (PP) analysis indicates 2.2-fold (22.9% vs 10.6%, p=0.305) and 1.6-fold (22.9% vs 14.1%, p=0.448) higher uptakes in arm A compared to arm B. Screening uptake varied between communities (range 0–62.1%). Among women who completed a questionnaire (18.3% in arm A, 21.7% in arm B), the screening uptake was 1.8-fold (ITT; p=0.1132) or 3-fold (PP; p<0.01) higher in arm A versus arm B.
Conclusions Pap and HPV self-sampling were compared in a marginalised, Canadian population. Results indicated a preference for self-sampling. More research on how to reach underscreened Indigenous women is necessary.
Trial registration number ISRCTN84617261.
- Indigenous health
- cervical screening
- Pap testing
- HPV self-sampling
- cluster randomized controlled trial
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Strengths and limitations of this study
The Anishinaabek Cervical Cancer Screening Study (ACCSS) addresses a health disparity in Canada, namely higher cervical cancer incidence in Indigenous compared to non-Indigenous women.
Participatory action research facilitated such a study for the first time in First Nations women in Northwest Ontario.
A randomised controlled trial comparing Papanicolaou versus HPV self-sampling was implemented in this population involving community lay health workers to recruit screening participants.
The longitudinal approach of the ACCSS will increase awareness for the benefit of cervical screening in underserved populations.
More collaborative work with the ACCSS partner communities based on innovative, community-shaped health promotions is necessary to elucidate ways in which to reach the women, who remain underscreened.
Cervical screening programmes, mainly based on Papanicolaou (Pap) testing, followed by management of detected precancerous cervical lesions, have reduced cervical cancer incidence and mortality in most developed countries by more than half.1 ,2 However, Indigenous populations worldwide continue to experience a disproportionate burden of cervical cancer morbidity and mortality.3 In Canada, women from First Nations, Métis and Inuit communities can have a 2-fold to 20-fold higher rate of cervical cancer than non-Indigenous women.4–6 This is probably a consequence of non-participation in screening or challenges with accessing follow-up care.7
Human papillomavirus (HPV) DNA testing has an overall greater sensitivity than traditional Pap cytology to detect lesions classified as cervical intraepithelial neoplasia grade 2 or higher.8 In addition, self-collection of these samples for HPV testing may help engage women who would not otherwise participate in standard-of-care Pap cervical screening due to factors such as geographical isolation and cultural sensitivity.9 ,10 In an Argentinian, cluster-randomised, controlled trial, self-sample collection resulted in a 4-fold higher screening uptake than HPV sampling performed by a clinician11 and we hypothesised that a similar approach could potentially increase screening uptake within Northwest Ontario First Nations communities that have above-average cervical cancer rates.12 ,13
In populations such as First Nations, community-based participatory action research (PAR) is recognised to be an appropriate approach as it focuses on community engagement, collaboration and reflection throughout the process design, implementation and dissemination of the research.14 ,15 Accordingly, the Anishinaabek Cervical Cancer Screening Study (ACCSS), using a unique mixed methods approach, was developed with 11 partner communities in Northwest Ontario, Canada. The main objective was to assess whether, within a PAR framework, screening participation increases in the First Nations population when HPV self-sampling instead of Pap testing is offered. After conducting interviews as well as focus groups with healthcare providers (HCPs) and women living on reserves about cervical cancer screening barriers (qualitative component),16–18 we performed a community-randomised controlled trial (quantitative component). Here, we present the results of the ACCSS quantitative component—the screening trial.
The method sections below are detailed according to CONSORT guidelines.19 ,20 The full trial protocol according to SPIRIT checklist21 ,22 is provided in online supplementary data 1. Model informed consent forms, as well as baseline and follow-up questionnaires, can be accessed in online supplementary data 2. Online supplementary table A summarises community events during the trial and online supplementary table B summarises the HPV typing results.
Supplementary data 1
Supplementary data 2
Supplementary data 3
We performed a two-arm, community-randomised controlled trial with 11 First Nations communities in the Thunder Bay district of Northwest Ontario, Canada. We stratified community clusters according to the total number of registered women. By conducting a stratified randomisation as detailed below, we intended for approximately equivalent numbers of women to be offered screening in each arm and to minimise bias introduced by preference of study participants or HCPs.
We identified 1002 eligible women (denominator) between 25 and 69 years old as being band members in one of the 11 participating First Nations communities and living on their own or another reserve, on Crown land or off-reserve with their main address in the Thunder Bay district (table 1).23 We excluded women who were pregnant from the study but arranged that they could participate after they had given birth. Participation in the trial could be through answering a baseline questionnaire (see online supplementary data 2) or through answering a baseline questionnaire and getting screened. As part of PAR,15 we hired and engaged with community-based research assistants (CBRAs) to facilitate the implementation of the screening modalities and collection of data in their respective communities.
The First Nations partner communities (11 clusters) each have between ∼70 and 800 band members and are scattered around the shores of Lake Superior and Lake Nipigon in Northwest Ontario, Canada.12 ,13 During an All Chiefs' meeting in Thunder Bay (November 2010) representing the Robinson Superior Treaty communities, to which Ingeborg Zehbe was invited, the then attending chiefs decided to participate in the proposed cervical screening study. Henceforth, any of these communities was eligible to join the study whereupon research agreements were ratified individually with each participating community between December 2010 and June 2011.
Communities were randomised to either the intervention group (arm A) or control group (arm B). Each arm consisted of a first offer of a screening modality in phase I lasting 3 months, followed by a 1–2-month intervention break, and finally the phase II cross-over period in which women were offered the alternate screening method. In the intervention group (arm A), women were first offered HPV testing using self-sampling and in the control group (arm B), women were first offered Pap testing. CBRAs invited women to participate in the ACCSS trial after an educational event and other recruitment strategies (see online supplementary table A). CBRAs obtained written informed (individual) consent from the participants and helped them to complete baseline and follow-up questionnaires.
For HPV DNA testing, the CBRAs provided the self-sampling kits and the participants were asked how they wished to be contacted in the event of a positive HPV test result. If a participant was found to have a high-risk infection (see online supplementary table B), she was referred for a follow-up (Pap test). For Pap testing, CBRAs scheduled appointments either directly with HCPs or through Well-Women clinics. Sample collection, processing, communication and follow-up were performed according to the Ontario Cervical Screening protocol for primary care practitioners.24
The primary outcomes of the trial were initial and cumulative screening uptakes as well as participant psychosocial status. Initial screening uptake was defined as the number of women who provided informed consent, completed a baseline questionnaire (online supplementary protocol appendix) and provided a self-sample or underwent a Pap test following the initial screening offer, divided by the eligible number of women registered in their community as of January 2014.23 For cumulative screening uptake, the numerator was defined as for initial uptake, except that participation was in either the initial or the subsequent screening offer; the denominator was defined as above. Psychosocial status included domains of worries/concerns about cancer, self-efficacy and external factors. A secondary outcome reported comfort with the screening experience.
Sample size and power estimations
When designing the ACCSS trial, data regarding cervical cancer screening rates in Ontario Indigenous women were not available. As First Nations women in Ontario are twice as likely to develop cervical cancer compared to the mainstream population,4 we calculated that the proportion of women eligible to be screened in First Nations communities in Northwest Ontario, and who had a Pap test in the previous 3 years, was half the general population uptake of 45%, that is, 22.5%. Previous studies report that between 32% and 58% (mean 45%) of women who have not had a Pap test during the last 6 years accepted an offer of self-sampling.25–27 We estimated three scenarios to illustrate the range of power we might have with a given sample size of ∼1000 women: (1) no change in screening uptake in the arm initially offered Pap testing, uptake 45% higher in the other arm, that is, 32.5%; (2) uptake in the arm initially offered Pap testing 30%, and 43.5% in the other arm; and (3) uptake in the arm initially offered Pap testing 45%, and 65.3% in the other arm. If clustering were disregarded in the power calculations, then, with ∼500 participants per arm, at 5% α level, the trial would have an average power of >96% to detect a difference in uptake for all three scenarios. Because of the potential for individuals' responses to the interventions within communities to be correlated, clustering had to be taken into account. Previous work on intracluster correlation (ICC) in the situation of variable cluster size28–30 suggests that the ICC would likely be less than the estimated maximum values (scenario 1: 0.0063, scenario 2: 0.0158, scenario 3: 0.0461), meaning that a sample size of 500 participants per arm would be adequate to achieve 80% power.
We stratified communities according to size (small, medium and large) and randomly assigned them to the first offer intervention type within their strata. Using band registration numbers from January 2013,13 we defined a small community as ≤360 total registered females, a medium community as between 361 and 910 total registered females and a large community as more than 910 total registered females. There were seven medium communities, three small communities and one large community. By conducting a stratified randomisation, we intended for approximately equivalent numbers of women to be offered screening in each arm. We randomised communities instead of individuals, to build on the community engagement element of the project as well as to reduce contamination between the control group (those who were first offered Pap testing) and the intervention group (those who were first offered the HPV self-test). A research assistant external to the Research Team performed the randomisation. First, we randomly assigned the seven medium sized communities. To do so, each community was assigned a random number and then those with the four highest numbers were placed into one arm and the remaining three into the other arm. We then applied the same approach to the three smaller communities. Finally, we placed the one large community in the arm with only three medium-sized communities in it.
Screening uptake was determined by intention-to-treat (ITT) and per protocol (PP) analyses, in recognition of limitations with both approaches.31–35 Thus, in the ITT analysis, women from a community that terminated its participation in the trial were included in the analysis as having no screening uptake. Women who did not answer the questionnaire were assumed not to have accepted the offer of screening. However, women who received a screening test that was not of the type offered were included as if they had accepted the offer of the test that had been assigned to their community. In the PP analysis, women from a community that terminated its participation in the trial were excluded from analysis. Women who did not answer the questionnaire were assumed not to have accepted the offer of screening and analysis was based on the type of test that the women had actually undergone. For cluster-level analysis, we compared the proportion of cervical cancer screening uptake between screening modalities using a permutation test.28 Percentage point (or ‘risk’) difference with a 95% CI was reported as calculated by the bootstrap method.36 To account for differences in questionnaire response, a proxy for CBRA outreach and community participation, additional analysis of the clustered screening uptake data was completed by restricting it to the subset of women who completed baseline questionnaires in each community.
The assessment of psychosocial status used a nine-item instrument with domains of worries/concerns about cancer, self-efficacy and external factors such as the community and relationships, based on similar published instruments.37 ,38 Each item was a seven-point Likert-type item. Psychosocial scores were obtained by calculating a mean score of all completed items for each respondent (after reverse-coding items 2, 4 and 8). We compared PP community-averaged baseline scores obtained at the time of intervention rollout in each community between the arms, irrespective of whether the women had accepted the offer of a screening test. We intended that this would reflect the response to the educational and engagement component of the intervention at the community level. We computed means and 95% CIs (as described above), and tested for the difference between the arms by a permutation test. In a descriptive analysis, we computed the scores at follow-up time points 1 and 2 between the arms, regardless of community, to assess psychosocial scores before and after screening. We also compared scores between screened and not screened women.
We applied standard descriptive statistics to the non-clustered data, sociodemographic characteristics, health status and health service use as well as comfort with either screening modality. We compared this information between the arms using the χ2 test, N-1 χ2 test for 2×2 tables with any low (<5) expected counts,39 the linear-by-linear association test for ordinal data,40 the Mantel-Haenszel χ2 test for stratified data, or the Welch's t-test for comfort scores. All statistical analyses were conducted using R V.3.1.0 (R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing. 2014. http://www.R-project.org/ (accessed 26 Jul 2015)) except for the linear-by-linear association test for which SPSS V.22 for Windows was used (IBM Corp. Released 2013. IBM SPSS Statistics for Windows, Version 22.0. Armonk, NY: IBM Corp. http://www-01.ibm.com/support/docview.wss?uid=swg21476197 (accessed 30 Apr 2015)). The α level was 0.05.
Six communities were randomised to arm A and the other five were randomised to arm B, with a total of 1002 eligible women. One community in arm B withdrew, resulting in 834 eligible women remaining (table 1).23 Enrolment in the trial was initiated in the communities between May and November 2013, with the initial screening offer being made in each community for a 3-month period, followed by a 1–2-month intervention break, and then the second offer of screening. Participant recruitment was completed in August 2014. Follow-up questionnaires were provided after 6 weeks following the completion of each 3-month screening round.
Using clustered ITT analysis (figure 1 and table 2), the community average uptake of screening in response to an initial offer of an HPV test based on self-sampling (arm A) was 20.0%, compared with 14.3% in the arm initially offered a Pap test (arm B): 1.4-fold increase, difference of 5.7% (95% CI −11.6 to 34.0, p=0.628).
The cumulative uptake of screening was 20.6% in arm A and 16.0% in arm B: 1.3-fold increase, difference of 4.6% (95% CI −13.9 to 32.3, p=0.694, permutation test). Compared to the a priori screening uptake estimate for Pap testing versus HPV self-sampling, the average screening uptake in phase I was lower than that was expected (estimated to be at least 22.5% vs 32.5%, respectively, ie, for scenario 1) but the difference in uptake between both modalities was close to the estimate (45%) at 40%.
Using clustered PP analysis (figure 1 and table 2), the average uptake of screening after the initial offer was 22.9% in arm A and 10.6% in arm B: 2.2-fold increase, difference of 12.3% (95% CI 2.4 to 46.6, p=0.305, permutation test). The cumulative uptake of screening was 22.9% in arm A and 14.1% in arm B: 1.6-fold increase, difference of 8.8% (95% CI −4.7 to 39.6, p=0.448, permutation test).
Compared to the a priori screening uptake estimate for Pap testing versus HPV self-sampling, the average screening uptake in phase I was lower than that was expected (estimated to be at least 22.5% vs 32.5%, respectively, ie, for scenario 1) but the difference in uptake between both modalities was higher than that was estimated (45%) at 96%.
Of the self-collected HPV test samples, 96.3% (78 of 81) were adequate for DNA analysis and of these, 19.2% tested positive for high-risk HPV types associated with cervical dysplasia (see online supplementary protocol and table 3B).
The proportion of screening uptake varied highly between communities/bands (table 1). At the end of phase I, the screening uptake ranged from 0.0% to 62.1% (ITT) or 2.9% to 62.1% (PP) among communities first offered self-sampling and from 0.0% to 47.1% (ITT) or 3.9% to 23.5% (PP) among communities first offered Pap testing. Interestingly, compared to our a priori screening uptake estimate, the best expected outcome (scenario 3) was reflected in one community of each respective arm using ITT analysis: 62.1% for HPV self-sampling (community 1) and 47.1% for Pap testing (community 7). Using PP analysis, this difference was 62.1% vs 23.5% in favour of HPV self-sampling. In addition, using PP analysis, community 2 in arm A was similar to scenario 1 with an uptake of 37%. Uptake in the other communities was all below the least estimated outcome (scenario 1). This diversity in uptake among communities motivated post hoc analyses to supplement those set forth as primary outcomes. By calculating the proportion of women who were screened in the subset of women who completed a questionnaire in phase I (18.3% in arm A and 21.9% in arm B), the screening uptake was higher for HPV self-sampling compared to Pap testing: 1.8-fold (33.8% difference) by ITT analysis; p=0.1132 or 3-fold (57.6% difference) by PP analysis; p<0.01.
Owing to the low participation rate in phase II, no further subgroup comparisons were attempted (figure 1). An age-covariate analysis was also originally planned, but was not possible given the lack of complete age data for non-respondent eligible women within each of the participating communities.
Psychosocial impact on women who were screened
For women who were screened and completed a follow-up questionnaire, mean psychosocial scores were not significantly different between baseline and follow-up times for arm A or arm B (p>0.05 by permutation test) (table 3A). For all time points and both study arms, summed psychosocial scores ranged between ‘slightly disagree’ and ‘neither agree nor disagree’, corresponding to scores between 2 and 4 on a seven-point Likert-type item.
Comfort level of women who were screened
As part of the follow-up questionnaires, women who got screened were asked about their comfort with the screening experience, ranging from very uncomfortable (score of 1) to very comfortable (score of 5) (table 3B). For arm A, women who self-sampled rated their mean comfort level as 4.23±0.83 SD (follow-up 1) and 4.26±1.02 SD (follow-up 2), indicating that they were comfortable to very comfortable with the experience. For Arm B, women who underwent Pap testing rated their mean comfort level as 3.75±1.21 SD (only data from follow-up 1 available), indicating that they were neutral to comfortable (p=0.125, Welch's t-test).
Characteristics of women who answered a baseline questionnaire
Socioeconomic and health demography characteristics were compared between the arms (tables 4 and 5). The age distributions were similar between the two arms, with the majority of women being <50 years of age. No significant differences in questionnaire responses between arms for sociodemographic or health characteristics were observed, apart from the difference in proportions of responders that lived on-reserve. This discrepancy is probably due to the fact that two communities still developing a land base for their band, and whose members by definition lived off-reserve, were both randomised to arm A.
While most questionnaire participants rated their health between ‘Good’ to ‘Excellent’, almost half of them stated their health was negatively impacted as a result of first-hand experience or familial encounters with residential schooling. Approximately two-thirds of all participating women reported that they had had a Pap test in the 3-year interval prior to completion of the baseline questionnaire. No significant difference in recent Pap history between arm A and B (p=0.796) or stratified according to women screened or not screened within arm A (p=0.756) and arm B (p=0.899) was noted, as assessed by the Mantel-Haenszel χ2 test.
The ACCSS is the first mixed methods study under a PAR framework to qualitatively and quantitatively investigate cervical screening behaviours of First Nations women in Canada.13 We have been mindful to engage the partner communities throughout the research process to initiate a cancer screening culture reflecting the Anishinaabek ‘Pimatisiwin’ (good life based on good health) philosophy which included the implementation of a cervical screening trial comparing two screening modalities. We hypothesised that in a marginalised population with high cervical cancer rates and low screening participation, HPV self-sampling may be an appealing alternative to Pap testing. We reasoned that empowering women to take their own samples may help overcome some of the structural and cultural barriers First Nations women face when attending cervical screening13—a re-affirmed notion and deemed to be culturally appropriate by our community informants during the qualitative phase of the ACCSS.17 ,18 DNA integrity from self-samples was excellent—an important factor to emphasise when promoting this screening method as some women reported being anxious that they were not taking their self-sample correctly. Greater than 95% of the samples could be HPV-tested and typed, confirming our earlier pilot data.12 Except for the higher employment rate, the sociodemographic characteristics of women who accepted the offer of screening and/or completed a questionnaire were similar to those of the region's Indigenous populations.41 ,42 For instance, using food security responses as a proxy, we noticed higher, self-reported, impoverished living conditions compared to the general population.43
The average screening uptake was less than a quarter for either screening modality, which may be related to not being aware of the benefit of screening, general fear of cancer and colonial legacy (eg, mistrust of the healthcare system). The estimated screening uptake was achieved only in two communities of arm A and in one community of arm B. Notably, the uptake in each participating community varied considerably, and only clustered PP analysis of screening uptake relative to the number of women in each community who completed a questionnaire showed statistical significance in favour of HPV self-sampling. However, the absolute difference in uptake in favour of HPV self-sampling was close to (ITT) or even twice (PP) our a priori estimation. A similar increase in uptake of HPV self-sampling over Pap testing was obtained for European and North American underscreened women,44 underscoring its benefits for cervical screening participation. A shift in the comfort level was noted in favour of HPV self-sampling in the current study. A deeper, qualitative analysis comparing HPV self-sampling with HCP-administered Pap cytology is still needed and is currently under way by the ACCSS team.
The large variability in the screening uptake within communities was equally noted for both arms, implying that the modality offered is not the only means to increase screening participation in our target population. Indeed, our data and the statement of one CBRA: “Easier for me, small community, I knew everyone who was in that age range”, suggest that the approach of using lay health workers for recruitment is more effective in smaller communities and needs to be further explored. Variation in the number of participants per community is also likely to have resulted from individual engagement of the CBRAs. In addition, being new in a community may affect the outcome: in the community with the lowest uptake, the CBRA was at first very shy to approach women but is now well trusted and enthusiastic to continue her work with the ACCSS.
Interestingly, no significant differences of psychosocial scores were found between the two screening arms. This finding may be explained by the fact that approximately two-thirds of our questionnaire participants reported having had a Pap test within the timeframe of 3 years recommended in recent guidelines45 and implies that we have primarily reached women who already are regularly screened. The ∼ 30% ‘underscreened’ women of our cohort were equally distributed in both arms. Participation recruitment included individual (eg, door-to-door visits and in-person appointments) and communal (eg, educational events, health fairs and well women clinics) strategies, and were most successful with women under the age of 50.
In conclusion, differences in uptake between both screening methods indicated a preference towards HPV self-sampling, but study participation was lower than what was expected. Long-term, the ACCSS with its longitudinal approach is poised to increase awareness for the benefit of cervical screening. In a recent gathering with selected members (HCPs and CBRAs) from all partner communities, the ACCSS team received positive feedback for this study and a request to continue the work. More collaborative work with the partner communities based on innovative, community-shaped health promotions and Indigenous knowledge-based education involving all ages and genders are necessary steps to elucidate ways in which to reach the women, who remain underscreened. For instance, in a recent focus group promoting arts-integrated education to explain the cause of cervical cancer through HPV, the women created their own HPV balls based on an electron micrograph of the HPV capsid molecule, “turning something ugly into something beautiful” as one participant expressed it.46
The authors thank the ACCSS First Nations partner communities in the Thunder Bay district, the CBRAs for their community engagement during both phases of the trial as well as the community management and leadership for their continuous support. They also thank Dr Pamela Wakewich and Mrs Melissa Togtema for critically reading the manuscript. Ann N Burchell is supported by a CIHR New Investigator Award. Gina Ogilvie holds a Canada Research Chair in Global Control of HPV-Related Disease and Cancer. Julian Little holds a Canada Research Chair in Human Genome Epidemiology.
Contributors IZ conceived and designed the study, completed all field work, including prior community engagement and formal community research agreements and led the writing of the manuscript. JL (senior author) contributed largely to the design of the ACCSS, supervised the analyses of the ACCSS trial data with IZ and contributed to writing and critical revision of the manuscript. RJ performed all statistical analyses and largely contributed to writing the manuscript. BW assisted in the field work and design of the questionnaires, as well as in the analyses and in revision of the manuscript. BW supervised the biostatistics and contributed to writing the manuscript. NE contributed to the clinical aspects of this project, the critical communication with CytoBase to obtain Pap screening data and revision of the manuscript. AS and MK contributed to the HPV analyses and writing of this portion of the manuscript. LB contributed to the community field work during the ACCSS trial, to the analyses and critically revised the manuscript keeping in mind cultural safety. KM contributed to the field work in her own community and critically revised the manuscript keeping in mind cultural safety. ANB initially designed the ACCSS trial, significantly contributed to the design of the questionnaires and revision of the manuscript. GO contributed to the design of the ACCSS trial and revision of the manuscript. All authors have read and approved the final version of the manuscript and agreed to act as guarantors of the work therein.
Funding This work was funded by the Canadian Institutes of Health Research (CIHR) with IZ being the nominated Principal Investigator (CIHR # MOP—119344). This project has been sponsored by the Thunder Bay Regional Research Institute (TBRRI; 980 Oliver Road, Thunder Bay ON, P7B 6V4). Neither the CIHR nor the TBRRI were involved with the design of the study, the analyses or the writing of the manuscript.
Competing interests The ACCSS team received support from Roche Diagnostics (RD) for the HPV analyses of the self-collected samples. However, RD was not involved with the study design, the analyses or the writing of the manuscript.
Ethics approval The trial (ISRCTN84617261) was approved by the Lakehead University Research Ethics Board (#126 12-13/ROMEO #1463139).
Provenance and peer review Not commissioned; externally peer reviewed.
Data sharing statement No additional data are available.