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Comparison of three tests for faecal calprotectin in children and young adults: a retrospective monocentric study
  1. Christine Prell1,
  2. Dorothea Nagel2,
  3. Folke Freudenberg1,
  4. Andrea Schwarzer1,
  5. Sibylle Koletzko1
  1. 1Division of Paediatric Gastroenterology and Hepatology, Dr. v. Haunersches Kinderspital, Ludwig-Maximilians-University, Munich, Germany
  2. 2Institute of Laboratory Medicine, Ludwig-Maximilians-University, Munich, Germany
  1. Correspondence to Dr Sibylle Koletzko; sibylle.koletzko{at}


Objective Faecal calprotectin is used as a sensitive marker for gastrointestinal mucosal inflammation. We compared the performance of three different assays in a large cohort of symptomatic paediatric patients.

Design Retrospective monocentric study.

Setting Inpatients and outpatients of a tertiary referral centre for paediatric gastroenterology.

Participants 304 symptomatic patients (163 males, aged 2–20 years) with active inflammatory bowel disease (IBD/A, n=130), IBD in clinical remission (IBD/R, n=62), other intestinal diseases (n=45) and controls without identified intestinal disease (n=67).

Interventions Calprotectin was measured in homogenised faecal samples with three tests (A: EliA Calprotectin, Phadia AB, Sweden; B: PhiCal, Calpro AS, Norway; C: EK-Cal, Bühlmann Laboratories, Switzerland).

Outcomes Concordance between tests was calculated using Kendall's τ coefficient.

Results IBD/A and controls were correctly classified as 97.7%/82.1% (A), 97.7%/85.1% (B) and 98.4%/62.7% (C; not significant). Test C tended to have higher calprotectin values with a lower specificity compared to tests A and B. The concordance between two tests was 0.835 for tests A and B, 0.782 for tests A and C and 0.765 for tests B and C.

Conclusions All three tests are very sensitive for detecting mucosal inflammation, but major differences exist between specificity and absolute values. It is highly advisable to use the test of the same manufacturer for follow-up and to monitor for disease activity.

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 3.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:

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