Article Text

Download PDFPDF

Association between the ACE insertion/deletion polymorphism and pterygium in Sardinian patients: a population based case–control study
  1. Paolo Demurtas1,
  2. Germano Orrù2,
  3. Pierpaolo Coni2,
  4. Luigi Minerba3,
  5. Michela Corrias1,
  6. Paola Sirigu1,
  7. Ignazio Zucca2,
  8. Elena Demurtas4,
  9. Cristina Maxia1,
  10. Franca Piras1,
  11. Daniela Murtas1,
  12. Simone Lai1,
  13. Maria Teresa Perra1
  1. 1Department of Biomedical Sciences, University of Cagliari Medical School, Cagliari, Italy
  2. 2Department of Surgical Science, University of Cagliari, Cagliari, Italy
  3. 3Department of Public Health, Clinical and Molecular Medicine, University of Cagliari, Cagliari, Italy
  4. 4Sant'Anna Surgery Clinic, Cagliari, Italy
  1. Correspondence to Dr Cristina Maxia; cmaxia{at}


Objective The purpose of the study was to examine whether the insertion (I) and/or deletion (D) polymorphism of ACE confers susceptibility to primary pterygium in Sardinian patients in a case–control study.

Methods and results Polymorphism genotyping was performed by nested PCR using genomic DNA extracted from the whole peripheral blood of participants with (n=251) and without (n=260) pterygium. DD, ID and II genotype frequencies were: 48%, 39% and 13%, respectively, for patients with pterygium, and 15%, 40% and 44%, respectively, for the control group. A statistically significant difference was found between the pterygium and control groups for the ACE I/D polymorphism (p<0.001). Moreover, a statistically significant difference was found between the DD and II groups (p<0.01; OR=10.49; 95% CI 6.18 to 17.79), DD+ID versus II group (p<0.01; OR=5.23; 95% CI 3.37 to 8.13) and DD versus ID groups (p<0.01; OR=3.21; 95% CI 2.04 to 5.04).

Conclusions Statistical analysis showed that the DD genotype is associated with an increased risk of developing pterygium, and with a good chance that the D allele may play an important role in the development of disease.


This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:

View Full Text

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.