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Neurology
Research
Graft-related disease progression in dura mater graft-associated Creutzfeldt-Jakob disease: a cross-sectional study
  1. Kenji Sakai1,
  2. Tsuyoshi Hamaguchi1,
  3. Moeko Noguchi-Shinohara1,
  4. Ichiro Nozaki1,
  5. Ichiro Takumi2,
  6. Nobuo Sanjo3,
  7. Yosikazu Nakamura4,
  8. Tetsuyuki Kitamoto5,
  9. Nobuhito Saito6,
  10. Hidehiro Mizusawa3,
  11. Masahito Yamada1
  1. 1Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan
  2. 2Department of Neurosurgery, Nippon Medical School Musashi Kosugi Hospital, Kawasaki, Japan
  3. 3Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
  4. 4Department of Public Health, Jichi Medical University, Shimotsuke, Japan
  5. 5Department of Prion Protein Research, Division of CJD Science and Technology, Tohoku University Graduate School of Medicine, Sendai, Japan
  6. 6Department of Neurosurgery, The University of Tokyo, Tokyo, Japan
  1. Correspondence to Professor Masahito Yamada; m-yamada{at}med.kanazawa-u.ac.jp

Abstract

Objectives Details of abnormal prion protein (PrPSc) propagation in the human central nervous system (CNS) are unclear. To assess the spread of PrPSc through the human CNS, we evaluated dura mater graft-associated Creutzfeldt-Jakob disease (dCJD) cases focusing on sites of grafting and dCJD pathological subtypes.

Design A cross-sectional study.

Setting nationwide surveillance data of human prion diseases in Japan over the past 12 years were applied for the study.

Participants Clinical data were obtained from 84 dCJD patients.

Outcome measures The clinical courses in cases of dCJD were analysed according to the grafting sites (supratentorial and infratentorial groups) and the pathological subtypes (non-plaque and plaque types).

Results Of the 84 cases of dCJD in this study, 36 (43%) were included in the supratentorial group and 39 (46%) were included in the infratentorial group. As initial manifestations, vertigo (p=0.007) and diplopia (p=0.041) were significantly more frequent in the infratentorial group than in the supratentorial group. During their clinical course, cerebellar signs appeared more frequently in the infratentorial group than in the supratentorial group (p=0.024). In the non-plaque type cases (n=53), the infratentorial group developed vertigo more frequently than the supratentorial group (p=0.017); moreover, cerebellar signs appeared more frequently in the infratentorial group (p=0.014). However, there was no significant difference between groups in the plaque type (n=18).

Conclusions The high frequency of clinical manifestations related to brain stem and cerebellar dysfunction in the non-plaque type dCJD with infratentorial grafting suggests that PrPSc commonly shows direct propagation into the CNS from contaminated dura mater grafts.

  • Infectious Diseases

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 3.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/3.0/

https://doi.org/10.1136/bmjopen-2013-003400

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    Article summary

    Article focus

    • Evaluation of the relationship between initial manifestation and the site of dura mater graft in patients with dura mater graft-associated Creutzfeldt-Jakob disease (dCJD).

    • We analysed the clinical data taking into account not only the grafting site but also the pathological subtypes of dCJD.

    Key messages

    • Infratentorial grafting cases in non-plaque type developed manifestations related to dysfunction of the brain stem and cerebellum more frequently than did supratentorial grafting cases.

    • Cerebellar signs also appeared more frequently in the infratentorial group during their clinical course.

    • Plaque-type cases showed no significant difference between the supratentorial and the infratentorial groups.

    Strengths and limitations of this study

    • This study suggests that the non-plaque-type abnormal prion protein (PrPSc) strain would propagate directly from the grafted dura mater to the adjacent brain and damage it earlier and more severely.

    • It would be difficult to determine focal brain lesions by PrPSc accumulation and subsequent neuronal damage from only information about clinical manifestations.

    • Another limitation of this study includes the relatively small number of plaque-type patients, which demonstrated no noteworthy results.

    Introduction

    Dura mater graft-associated Creutzfeldt-Jakob disease (dCJD) is an acquired Creutzfeldt-Jakob disease (CJD) related to previous dura mater graft transplantation.1 ,2 Details of abnormal prion protein (PrPSc) propagation in the human central nervous system (CNS) are not fully understood.3 Previous studies of dCJD cases disclosing the relationship between initial manifestation and the site of grafting proposed that PrPSc may propagate directly from the contaminated dura mater graft to the adjacent brain regions and spread from the initially infected regions to other brain regions.4–6 In previous studies of the dCJD case series, however, results were obtained without considering the dCJD pathological subtypes.5 ,6

    There are two subtypes of dCJD: non-plaque type and plaque type. The non-plaque type is characterised by typical clinical features of CJD and synaptic-type PrPSc deposits without PrPSc plaques in the brain. In contrast, the plaque type is characterised by atypical clinical features and plaque-type PrPSc deposits in the brain.7–9 These subtypes arise from two distinct prion strains.7–9 Each prion strain has different characteristics of incubation period and neuropathological features when inoculated into defined inbred mice8 ,10; it was proposed that each prion strain must show a distinct propagation process in the human brain.10 Therefore, the disease pathological subtypes (prion strains) should be taken into consideration when clinical features are analysed in dCJD cases. This study made use of the prospective prion disease surveillance in Japan to analyse clinical manifestations of dCJD cases taking into account not only their grafting sites but also their pathological subtypes.

    Materials and methods

    Patients

    In Japan, the prospective surveillance of human prion disease by the CJD Surveillance Committee in Japan started in April 1999. Details of the Japanese surveillance system and case definition were reported previously.11 Briefly, all patients suspected of having a prion disease were registered by the CJD Surveillance Committee and their diagnoses were judged. Prion diseases were classified into four categories: (1) sporadic CJD, (2) acquired prion diseases (iatrogenic CJD or variant CJD), (3) genetic prion diseases and (4) unclassified prion disease. Sporadic CJD was diagnosed according to the classical criteria established by Masters et al.12 The WHO criteria (WHO, 1998)13 were applied from April 2009. Regarding the patients with previous medical procedures which might be related to CJD, details of the information were collected and the diagnosis of iatrogenic CJD was decided carefully. Iatrogenic CJD was also diagnosed and categorised (definite, probable and possible) using the criteria for sporadic CJD. In patients with iatrogenic CJD, the diagnosis of dCJD was decided by confirmation of dura mater grafting. The medical records, information from each neurosurgeon or the autopsy findings were used to ensure the occurrence of dura mater grafting. Cases of dCJD were categorised into two pathological subtypes: the non-plaque type, which shows synaptic-type PrPSc deposits without PrPSc plaques; and the plaque type, which shows plaque-type PrPSc deposits.7 In cases without pathological confirmation, we classified cases showing periodic sharp-wave complexes in the EEG within 12 months of disease onset as non-plaque type and cases showing no periodic sharp-wave complexes in the EEG within 12 months of disease onset as plaque type.7 As the plaque-type dCJD patients without pathological confirmation never satisfied the criteria for probable case,7 possible cases were included in this analysis in addition to definite and probable cases. We analysed all dCJD patients identified by the current surveillance system up to and including February 2012.

    Written informed consent to participate in this study was obtained from the families of all the patients.

    Clinical studies

    We collected the following information regarding dura mater grafting: calendar year when the surgical operation was performed, the brand name of the dura mater graft and the site of the dural grafting. In addition to the grafting sites (supratentorial or infratentorial), we also analysed the following parameters: sex; age at dural grafting; incubation period; age at CJD onset; information about initial manifestation and symptoms which appeared during their clinical course, including cerebellar signs, psychiatric features, dementia, visual disturbances, myoclonus, extrapyramidal signs and pyramidal signs. Since several patients developed more than one initial manifestation, we counted each manifestation separately. Information about PrP gene polymorphisms at codons 129 and 219 was also collected from patients who underwent genetic analysis. Moreover, we analysed the initial symptoms and clinical manifestations that emerged during their clinical course in each pathological subtype, and compared the supratentorial grafting cases with the infratentorial grafting cases.

    Statistical analysis

    Any difference in age at dural grafting, incubation period and age at CJD onset between the supratentorial group and the infratentorial group was assessed using the Mann-Whitney U test. Pathological subtype classification, sex, initial symptoms, clinical manifestations and PrP gene polymorphisms were assessed using a χ2 test or Fisher's exact probability test. Statistical significance was defined as p<0.05. Statistical analyses were performed using SPSS V.19 (IBM, Armonk, New York, USA).

    Results

    The CJD Surveillance Committee in Japan identified 84 patients with dCJD between April 1999 and February 2012. Fifty-eight patients with dCJD had already been reported by previous investigations9 ,11; therefore, the total number of dCJD cases was 142.2 The surgeries with dura mater grafts were performed between 1975 and 1993. The brand name of grafted dura mater was identified in 74 cases (88%). Lyodura (B Braun, Melsungen, Germany) was transplanted in all proven cases. The grafting site was confirmed in 77 of 84 cases (92%), 36 in supratentorial regions, 39 in infratentorial regions and 2 in spinal cord regions (table 1). An autopsy was performed in 32 cases (38%).

    View this table:
    Table 1

    Clinical features of the dura mater graft-associated Creutzfeldt-Jakob disease cases for all cases, the supratentorial group and the infratentorial group

    The 84 dCJD cases were classified into 53 cases (63%) of the non-plaque type and 18 cases (21%) of the plaque type. It was not possible to classify the pathological subtype in 13 cases (16%) due to an inadequacy of clinical or pathological information (table 1). There were 18 of 53 non-plaque-type cases (34%) proven by autopsy and 14 of 18 plaque type (78%) cases proven by autopsy.

    The clinical features of dCJD for all patients, the supratentorial group and the infratentorial group are summarised in table 1. The proportion of women in the infratentorial group was larger than that in the supratentorial group (p=0.015). Age at dural grafting, incubation period or age at CJD onset showed no significant difference between the two groups. Regarding initial manifestations, vertigo (31% and 3%; p=0.007) and diplopia (15% and 0%; p=0.041) were more frequently observed in the infratentorial group than in the supratentorial group. Dementia and behavioural abnormality suggesting dysfunction of the cerebrum demonstrated no significant difference between the groups. In the infratentorial group, eight cases (31%) developed dementia or behavioural abnormalities. The incubation periods of cases developing dementia or behavioural abnormalities in the supratentorial group and the infratentorial group, reported as the median (range), were 15 (11–30) years and 16 (10–25) years, respectively (p=0.847). During the clinical course, the infratentorial group showed cerebellar signs (87% and 64%; p=0.024) more frequently than did the supratentorial group. There was no significant difference in the proportion of the PrP genotype or the type classification of dCJD between the two groups. In addition, two cases with spinal cord region grafting developed dementia, diplopia or unsteady gait.

    Results from the analysis of the non-plaque-type cases were similar to those of the sample population as a whole (table 2). Vertigo was more frequently observed as an initial manifestation in the infratentorial group than in the supratentorial group (32% and 0%; p=0.017). There was a trend in the increase of diplopia frequency in the infratentorial group (21% and 0%; p=0.074). Dementia and behavioural abnormalities demonstrated no significant difference between the two groups. In the infratentorial group, seven cases (37%) demonstrated dementia or behavioural abnormalities as initial manifestations. Similar to the analysis of the sample population as a whole, the median incubation period of cases developing dementia or behavioural abnormalities showed no significant difference between the supratentorial and infratentorial groups (data not shown). Cerebellar signs were realised significantly more often in the infratentorial group during their clinical course than in the supratentorial group (87% and 50%; p=0.041). In contrast, there was no significant difference between the supratentorial group and the infratentorial group concerning initial manifestations or manifestations during their clinical course in the analysis of the plaque-type cases (table 3).

    View this table:
    Table 2

    Clinical manifestations of non-plaque-type cases

    View this table:
    Table 3

    Clinical manifestations of plaque-type cases

    Discussion

    In this study of dCJD cases, we have reported that infratentorial grafting cases in not only the sample population as a whole but also the non-plaque-type cases developed manifestations related to dysfunction of the brain stem and cerebellum more frequently than did the supratentorial grafting cases. Moreover, cerebellar signs appeared more frequently in the infratentorial group during their clinical course. In contrast, plaque-type cases showed no significant difference between the supratentorial and the infratentorial groups.

    These results suggest that the non-plaque-type PrPSc strain would propagate directly from the grafted dura mater to the adjacent brain. In experimental studies, PrPSc has the ability to spread from cell to cell.3 Mice experiments with PrPSc inoculated directly into the brain showed that PrPSc accumulated at the site of initial inoculation and spread around that area.14–16 A mouse model of dCJD, in which a small collagen sheet absorbing prion-infected brain homogenates was transplanted onto the brain surface, also disclosed spongiform changes and accumulation of PrPSc in the transplanted cortical areas.17 Concerning the plaque-type prion strain, there was no significant difference between the infratentorial grafting and supratentorial grafting groups. The plaque-type PrPSc strain may have a propagation process that is distinct from the non-plaque-type prion strain. A case series study of dCJD demonstrated that plaque-type patients were likely to develop gait disturbance.7 In this study, three of four plaque-type patients developed unsteady gait after supratentorial grafting (table 3). These results suggest that the plaque-type PrPSc strain, which must have a distinct nature from the non-plaque-type PrPSc strain, might damage specific brain regions causing gait disturbance during the early stage of the disease process in spite of the difference in grafting sites, possibly through transportation of PrPSc to the specific brain regions. An animal study proved that the plaque-type dCJD could be caused by cross-sequence transmission of sporadic CJD VV2 prions to individuals that are methionine homozygotes at codon 129 of the PrP gene.8 Furthermore, another animal study showed that sporadic CJD MV2 prions could also induce plaque-type dCJD pathology (TK unpublished data). CJD VV2 and CJD MV2, which might cause plaque-type prions, are well known as ataxic forms of CJD.18 Meanwhile, the small number of plaque-type cases might influence these results, resulting in no significant difference between the supratentorial and the infratentorial groups.

    Our study suggests that generally brain tissue near the grafting site was damaged earlier and more severely through direct propagation of PrPSc from the grafts. However, some cases have also suggested that there were different patterns of PrPSc propagation. For instance, 31% of all infratentorial grafting cases and 37% of the non-plaque type with infratentorial grafting cases developed dementia or behavioural abnormalities, indicating initial dysfunction of the cerebrum. Moreover, two cases of spinal cord grafts did not develop symptoms related to spinal cord dysfunction. Interestingly, the cerebellar signs throughout the clinical course in the supratentorial group were demonstrated less frequently than those in the infratentorial group in all cases and in the non-plaque-type cases.

    These results may suggest the presence of different propagation pathways of PrPSc in addition to the direct invasion of brain tissue, via the cerebrospinal fluid, bloodstream or lymphatic drainage from the CNS. Recently, it has been suggested that lymphatic systems could play an important role in the PrPSc infection of the brain.19 In most cases of prion infection regarding variant CJD, the point of PrPSc entry can be outside the nervous system.19 After the infection of the organs outside the nervous system, PrPSc moves into the blood and lymphoid fluids and replicates in the lymphoid organs. PrPSc is then transported to the brain through the peripheral nervous system.19 ,20 In addition to variant CJD cases, PrPSc was detected in the muscles, intramuscular nerve fibres and dorsal root ganglia in sporadic CJD, in which causes of the disease are uncertain.21–23 PrPSc contamination of dural grafts may have a similar process of indirect infection of the CNS in addition to the direct invasion of the adjacent brain tissue. Although little data regarding PrPSc deposition in tissues other than the CNS in dCJD are available, PrPSc was detected in the peripheral nerves in cases of dCJD in an immunohistochemical study and western blot analysis.24 In this study, no significant difference in the incubation period was revealed between the patients with suggested direct infection and the patients with suggested indirect infection. Further examination regarding PrPSc accumulation in other organs is necessary to confirm this indirect infection of PrPSc into the CNS in dCJD cases.

    In addition to a hypothesis of indirect propagation of PrPSc into the CNS, various combinations of clinical manifestations and brain lesions may influence the symptoms of dCJD patients. Ataxia is a common manifestation stemming from cerebellar or brain stem dysfunction; however, lesions involving cerebral areas and fibres connecting to the cerebellum also cause ataxia.25 Moreover, several case reports presented patients showing rotational vertigo in the clinical course of stroke in the cerebral hemisphere.26 It would be difficult to determine focal brain lesions by PrPSc accumulation and subsequent neuronal damage from only information about the clinical manifestations in each case. Therefore, analyses with imaging techniques, including MRI, single-photon emission tomography and positron emission tomography, are necessary to confirm the relationship between the grafting site and PrPSc propagation in human CNS.

    In conclusion, our results indicate that PrPSc of non-plaque-type dCJD tends to spread from the grafted sites to the adjacent brain, although different propagation pathways may be present in some cases.

    Acknowledgments

    The authors would like to thank the members of the Creutzfeldt-Jakob disease Surveillance Committee in Japan; Creutzfeldt-Jakob disease specialists in the prefectures and doctors, as well as patients with Creutzfeldt-Jakob disease and their families for providing clinical information about the patients. They also thank Dr Takeshi Sato for his insightful comments and Ms Kazuko Sawada for her data collection and secretarial assistance.

    References

    1. ↵
      Centers for Disease Control (CDC). Rapidly progressive dementia in a patient who received a cadaveric dura mater graft. MMWR Morb Mortal Wkly Rep 1987;36:49–50, 55.
      OpenUrlPubMed
    2. ↵
      1. Brown P,
      2. Brandel J,
      3. Sato T,
      4. et al
      . Iatrogenic Creutzfeldt–Jakob disease, final assessment. Emerg Infect Dis 2012;18:901–7.
      OpenUrlCrossRefPubMed
    3. ↵
      1. Wadsworth JDF,
      2. Collinge J
      . Molecular pathology of human prion disease. Acta Neuropathol 2011;121:69–77.
      OpenUrlCrossRefPubMedWeb of Science
    4. ↵
      1. Nishida Y,
      2. Yamada M,
      3. Hara K,
      4. et al
      . Creutzfeldt–Jakob disease after Jannetta's operation with cadaveric dura mater graft: initial manifestations related to the grafted site. J Neurol 2002;249:480–3.
      OpenUrlCrossRefPubMedWeb of Science
    5. ↵
      1. Sato T,
      2. Masuda M,
      3. Utsumi Y,
      4. et al
      . Dura mater related Creutzfeldt-Jakob disease in Japan: relationship between sites of grafts and clinical features. In: Kitamoto T ed. Prions: food and drug safety. Tokyo: Springer-Verlag, 2005:31–40.
    6. ↵
      1. Heath CA,
      2. Barker RA,
      3. Esmonde TFG,
      4. et al
      . Dura mater-associated Creutzfeldt–Jakob disease: experience from surveillance in the UK. J Neurol Neurosurg Psychiatry 2006;77:880–2.
      OpenUrlAbstract/FREE Full Text
    7. ↵
      1. Noguchi-Shinohara M,
      2. Hamaguchi T,
      3. Kitamoto T,
      4. et al
      . Clinical features and diagnosis of dura mater graft-associated Creutzfeldt–Jakob disease. Neurology 2007;69:360–7.
      OpenUrlCrossRef
    8. ↵
      1. Kobayashi A,
      2. Asano M,
      3. Mohri S,
      4. et al
      . Cross-sequence transmission of sporadic Creutzfeldt–Jakob disease creates a new prion strain. J Biol Chem 2007;282:30022–8.
      OpenUrlAbstract/FREE Full Text
    9. ↵
      1. Yamada M,
      2. Noguchi-Shinohara M,
      3. Hamaguchi T,
      4. et al
      . Dura mater graft-associated Creutzfeldt–Jakob disease in Japan: clinicopathological and molecular characterization of the two distinct subtypes. Neuropathology 2009;29:609–18.
      OpenUrlCrossRefPubMedWeb of Science
    10. ↵
      1. Collinge J,
      2. Clarke AR
      . A general model of prion strains and their pathogenicity. Science 2007;318:930–6.
      OpenUrlAbstract/FREE Full Text
    11. ↵
      1. Nozaki I,
      2. Hamaguchi T,
      3. Sanjo N,
      4. et al
      . Prospective 10-year surveillance of human prion diseases in Japan. Brain 2010;133:3043–57.
      OpenUrlAbstract/FREE Full Text
    12. ↵
      1. Masters CL,
      2. Harris JO,
      3. Gajdusek DC,
      4. et al
      . Creutzfeldt–Jakob disease: patterns of worldwide occurrence and the significance of familial and sporadic clustering. Ann Neurol 1979;5:177–88.
      OpenUrlCrossRefPubMedWeb of Science
    13. ↵
      WHO. Global surveillance, diagnosis and therapy of human transmissible spongiform encephalopathies. Report of a WHO consultation [eLetter] February 1998. http://www.who.int/csr/resources/publications/bse/WHO_EMC_ZDI_98_9/en/
    14. ↵
      1. Tateishi J,
      2. Nagara H,
      3. Hikita K,
      4. et al
      . Amyloid plaques in the brains of mice with Creutzfeldt–Jakob disease. Ann Neurol 1984;15: 278–80.
      OpenUrlCrossRefPubMedWeb of Science
    15. ↵
      1. Durand-Gorde JM,
      2. Bert J,
      3. Nieoullon A
      . Changes in tyrosine hydroxylase, glutamic acid decarboxylase and choline acetyltransferase after local microinoculation of scrapie agent into the nigrostriatal system of the golden hamster. Brain Res 1985;341:243–51.
      OpenUrlCrossRefPubMedWeb of Science
    16. ↵
      1. Kordek R,
      2. Hainfellner JA,
      3. Liberski PP,
      4. et al
      . Deposition of the prion protein (PrP) during the evolution of experimental Creutzfeldt–Jakob disease. Acta Neuropathol 1999;98:597–602.
      OpenUrlCrossRefPubMed
    17. ↵
      1. Furuya K,
      2. Kawahara N,
      3. Yamakawa Y,
      4. et al
      . Intracerebroventricular delivery of dominant negative prion protein in a mouse model of iatrogenic Creutzfeldt-Jakob disease after dura graft transplantation. Neurosci Lett 2006;402:222–6.
      OpenUrlPubMed
    18. ↵
      1. Puoti G,
      2. Bizzi A,
      3. Forloni G,
      4. et al
      . Sporadic human prion diseases: molecular insights and diagnosis. Lancet Neurol 2012;11:618–28.
      OpenUrlCrossRefPubMedWeb of Science
    19. ↵
      1. Aguzzi A,
      2. Kana V
      . Introduction to prion disorders. In: Dickson DW, Weller RO. eds. Neurodegeneration: the molecular pathology of dementia and movement disorders. 2nd edn. Chichester: Wiley-Blackwell, 2011:315–21.
    20. ↵
      1. Glatzel M,
      2. Heppner FL,
      3. Albers KM,
      4. et al
      . Sympathetic innervation of lymphoreticular organs is rate limiting for prion neuroinvasion. Neuron 2001;19:25–34.
      OpenUrl
    21. ↵
      1. Glatzel M,
      2. Abela E,
      3. Maissen M,
      4. et al
      . Extraneural pathologic prion protein in sporadic Creutzfeldt-Jakob disease. N Engl J Med 2003;349:1812–20.
      OpenUrlCrossRefPubMedWeb of Science
    22. ↵
      1. Head MW,
      2. Ritchie D,
      3. Smith N,
      4. et al
      . Peripheral tissue involvement in sporadic, iatrogenic, and variant Creutzfeldt-Jakob disease: an immunohistochemical, quantitative, and biochemical study. Am J Pathol 2004;164:143–53.
      OpenUrlPubMedWeb of Science
    23. ↵
      1. Peden AH,
      2. Ritchie DL,
      3. Head MW,
      4. et al
      . Detection and localization of PrPSc in the skeletal muscle of patients with variant, iatrogenic, and sporadic forms of Creutzfeldt-Jakob disease. Am J Pathol 2006;168:927–35.
      OpenUrlCrossRefPubMedWeb of Science
    24. ↵
      1. Ishida C,
      2. Okino S,
      3. Kitamoto T,
      4. et al
      . Involvement of the peripheral nervous system in human prion diseases including dural graft associated Creutzfeldt-Jakob disease. J Neurol Neurosurg Psychiatry 2005;76:325–9.
      OpenUrlAbstract/FREE Full Text
    25. ↵
      1. Wood NW,
      2. Harding AE
      . Ataxic disorders. In: Bradley WG, Daroff RB, Fenicbel GM, et al, eds. Neurology in clinical practice. 3rd edn. Boston: Buttermorth-Heinemann, 2000:309–17.
    26. ↵
      1. Naganuma M,
      2. Inatomi Y,
      3. Yonehara T,
      4. et al
      . Rotational vertigo associated with parietal cortical infarction. J Neurol Sci 2006;246:159–61.
      OpenUrlCrossRefPubMed

    Footnotes

    • Contributors KS participated in the study concept and design, as well as in the analysis and interpretation of the data, statistical analysis, drafting/revision of the manuscript. TH participated in the study concept and design, as well as in the drafting/revision of the manuscript. MN-S, IN, IT, NSanjo, NSaito and TK participated in the acquisition of the data and revision of the manuscript. YN participated in the acquisition of the data, statistical analysis and revision of the manuscript. HM participated in the acquisition of the data, revision of the manuscript and study supervision. MY participated in the study concept and design, acquisition of the data, drafting/revision of the manuscript and study supervision.

    • Funding This work was supported by the following grants: the Research Committee of Prion Disease and Slow Virus Infection, the Ministry of Health, Labour and Welfare of Japan; and the Research Committee of Surveillance and Infection Control of Prion Disease, the Ministry of Health, Labour and Welfare of Japan.

    • Competing interests None.

    • Ethics approval This study was conducted with the approval of the institutional ethics committee at Kanazawa University and Tokyo Medical and Dental University.

    • Provenance and peer review Not commissioned; internally peer reviewed.

    • Data sharing statement No additional data are available.