Article Text

Study protocol of LANTana: a phase Ib study to investigate epigenetic modification of somatostatin receptor-2 with ASTX727 to improve therapeutic outcome with [177Lu]Lu-DOTA-TATE in patients with metastatic neuroendocrine tumours, UK
  1. Ravindhi Murphy1,
  2. Gurvin Chander2,
  3. Maria Martinez1,
  4. Caroline Ward3,
  5. Sairah R Khan4,
  6. Mitesh Naik4,
  7. Tara Barwick5,6,
  8. Eric Aboagye3,
  9. Rohini Sharma1
  1. 1Department of Surgery and Cancer, Hammersmith Hospital, London, UK
  2. 2Department of Surgery and Cancer, Birmingham Women's and Children's NHS Foundation Trust, Birmingham, UK
  3. 3Department of Surgery and Cancer, Imperial College London, London, UK
  4. 4Department of Nuclear Medicine, Hammersmith Hospital, London, UK
  5. 5Department of Cancer and Surgery, Imperial College London, London, UK
  6. 6Department of Radiology, Imperial College Healthcare NHS Trust, London, UK
  1. Correspondence to Dr Rohini Sharma; r.sharma{at}


Introduction Suitability for peptide receptor radionuclide therapy (PRRT) for neuroendocrine neoplasia (NENs) depends on presence of somatostatin receptor-2 (SSTR2) determined by [68Ga]Ga-DOTA-peptide-positron emission tomography (PET). Some patients have low or no uptake on [68Ga]Ga-DOTA-peptide-PET, precluding PRRT. The upstream promoter region of SSRT2 is methylated, with percentage of methylation correlating with SSTR2 expression. Demethylating agents increase uptake on PET imaging in vivo such that tumours previously negative on PET become positive, correlating with a dose dependent increase in tumorous SSTR2 expression. LANTana will determine whether treatment with the demethylating agent, ASTX727, results in re-expression of SSTR2 using [68Ga]Ga-DOTA-peptide-PET to image epigenetic modification of the SSTR2 locus, allowing subsequent PRRT.

Methods and analysis 27 participants with a histological diagnosis of NEN (Ki67<55%) with no or low uptake on baseline [68Ga]Ga-DOTA-TATE-PET/CT will be recruited. Patients will receive 5 days of ASTX727 (fixed dose 35 mg decitabine+100 mg cedazuridine). [68Ga]Ga-DOTA-peptide-PET/CT will be repeated day 8±2; where there is significant uptake greater than liver in most lesions, PRRT will be administered. Primary objective is to determine re-expression of SSTR2 on PET imaging. Tolerability, progression-free survival, overall response and quality of life will be assessed. Methylation in peripheral blood mononuclear cells and tumorous methylation will be evaluated.

Ethics and dissemination LANTana has ethical approval from Leeds West Research Ethics Committee (REC Reference: 21/YH/0247).

Sponsored by Imperial College London and funded by Advanced Accelerator Applications pharmaceuticals. Results will be presented at conferences and submitted to peer-reviewed journals for publication and will be available on

Trial registration numbers EUDRACT number: 2020-003800-15, NCT05178693.

  • Hepatobiliary tumours
  • Adult oncology

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  • WIll investigate epigenetic modification to resensitise tumours to somatostatin receptors (SSTR2)-targeting radiotheranostic drugs.

  • Prospective trial using a demethylation strategy to re-express SSTR2 using [68Ga]Ga-DOTA-PET as a biomarker of response

  • May potentially allow treatment with [177Lu]Lu-DOTA-TATE in a group of patients who otherwise would be excluded.


While thought to be a rare cancer, the incidence of neuroendocrine neoplasias (NENs) has increased dramatically (approximately 500 fold) over the past 30 years, and unlike most other solid tumours, the incidence continues to rise.1 NENs are a heterogeneous group of tumours derived from peptide and amine producing cells of the neuroendocrine system, characterised by neurosecretory granules containing a variety of hormones and biogenic amines. NENs can arise anywhere in the body, commonly midgut (40%) and pancreas (8%).2 NENs can also arise in the lung and often the primary is unknown.1 Some release vasoactive substances into systemic circulation, causing diarrhoea, flushing and wheezing, having a negative impact on quality of life (QoL). However, the majority (>80%) are non-secretory, and often present with widespread metastatic disease. The management and prognosis of NENs are determined by both stage and grade. For patients with localised or limited disease, the primary modality of therapy is surgery, aiming to cure. However, in patients with metastatic disease, the only option is systemic therapy, administered with palliative intent. Tumour grade is determined by mitotic count or Ki67 proliferation index: low-grade or grade 1: Ki67<3%, moderate or grade 2: Ki67 between 3% and 20% (collectively termed neuroendocrine neoplasia, NENs), and high-grade or grade 3: Ki67>20%. There is increasing recognition of heterogeneity in grade 3 NENs such that poorly differentiated tumours with Ki67>55% are defined as neuroendocrine carcinomas (NECs), and have an average survival of 5 months while well-differentiated grade 3 NENs have a treatment response and outcome analogous to grade 2 NENs.3 4 The prognosis for metastatic NENs varies from 25% 5-year survival in grade 1 NENs to<12 months in NECs.5

NENs are characterised by the presence of somatostatin receptors (SSTRs) on the tumour surface.6 The SSTR family comprises five widely distributed G-protein coupled receptors that mediate intracellular signalling pathways with roles in cell proliferation, cell differentiation and angiogenesis.7 The expression of the SSTRs on NENs can be exploited for therapeutic benefit with somatostatin analogues (SSAs) and Peptide Receptor Radionuclide Therapy (PRRT). Indeed, the long-acting SSA preparations octreotide and lanreotide are considered standard of care for the treatment of grade 1 and 2 NENs within the current European Neuroendocrine Tumour Society Consensus Guidelines, with proven antiproliferative effects in phase 3 clinical trials.8–10 SSA analogues can also be radiolabelled, both to stage NENs using either scintigraphy or positron emission tomography (PET), and to deliver targeted radiotherapy (PRRT).

PRRT consists of a SSA linked to a long-acting beta-emitting radionuclide, such as lutetium 177, which has a half-life of 140 days and particle range of up to 2 mm ([177Lu]Lu-DOTA0-Tyr3-Octreotate, [177Lu]Lu-DOTA-TATE). The radiolabelled SSA binds specifically to SSTR2 on the tumour surface. The complex is internalised delivering selective, targeted radiotherapy. The efficacy of [177Lu]Lu-DOTA-TATE was highlighted in the NETTER-1 trial which has arguably provided the biggest clinical impact in the treatment of SSTR2-expressing NENs in terms of improvement in clinical outcomes.11 In NETTER-1, patients with metastatic grade 1–2 NENs were randomised to receive either high dose octreotide or [177Lu]Lu-DOTA-TATE. The trial reported a significant reduction in progression or death, 79%, with [177Lu]Lu-DOTA-TATE over SSAs (HR 0.21, 95% CI 0.13 to 0.34; p<0.001).11 Importantly, treatment with PRRT improved QoL.12 The use of PRRT is approved by most healthcare systems for the management of unresectable or metastatic, progressive, well-differentiated (grade 1 or grade 2), SSTR-positive NENs.

Selection for treatment with PRRT is based on the presence of SSTR2 on the tumour as illustrated by positive receptor imaging; typically using [68Ga]Ga-labelled SSAs ([68Ga]Ga-DOTA-peptides) PET/CT imaging.13 The only validated predictor of response to PRRT is positive SSTR2 imaging, such that patients with no tumorous uptake or tumorous uptake below that of background liver on imaging are not suitable for PRRT.14 15 While grade 1 tumours are well differentiated and retain tumour expression of SSTR2, some intermediate and high-grade NENs either have low or no uptake on [68Ga]Ga-DOTA-peptide-PET/CT or heterogeneous expression. In contrast, these patients have positive uptake on 2-[fluorine-18]fluoro-2-deoxy-d-glucose PET/CT and are indicative of a group of patients with poor prognosis who do not benefit from PRRT.16 17

Previous studies have demonstrated that the expression of the SSTR2 receptor is controlled by epigenetic modifications of a novel SSTR2 upstream promoter.18 This putative upstream promoter area for SSTR2 is conserved across species and is responsible for between 40% and 60% of total SSTR2 production across multiple cell lines representing different cancer types. Methylation of this promoter was demonstrated to be reversible in vitro with the first-generation DNA methyltransferase inhibiting agent decitabine.18 These findings were extended by Veenstra et al and Taelman et al who illustrated that treatment of NEN cell lines with decitabine, not only resulted in re-expression of SSTR2 but importantly resulted in enhanced uptake of radiolabelled octreotide.19 20 In order to assess if re-expression of SSTR2 results in improved cytotoxicity of [177Lu]Lu-DOTA-TATE, Taelman incubated cells with [177Lu]Lu-DOTA-TATE following treatment with decitabine. The authors report increased cytotoxicity with combination therapy compared with [177Lu]Lu-DOTA-TATE alone.19 We extended these findings into a mouse model of NEN using BON-1 cells (low basal expression SSTR2) and QGP-1 cells (high basal expression SSTR2) using guadecitabine to illustrate a 70% increase in the SSTR2-directed radioligand, [18F]-FET-βAG-TOCA in BON-1 tumour model.21 22 We therefore demonstrated that PET imaging can be used as a biomarker of demethylation of SSTR2.21 Taken together, this suggests that SSTR2 epigenetic silencing can be reversed, opening a novel therapeutic approach for patients with no or marginal uptake on [68Ga]Ga-DOTA-PET scans who are currently not suitable for PRRT, using [68Ga]Ga-DOTA-PET as a biomarker of SSTR2 re-expression.

The primary aim of the LANTana is to determine whether pretreatment with the demethylating agent ASTX727 results in re-expression of SSTR2 in patients with metastatic NENs who have no or low expression of SSTR2 on [68Ga]Ga-DOTA-peptide-PET/CT. [68Ga]Ga-DOTA-peptide-PET/CT will be used to image epigenetic modification of the SSTR2 locus allowing subsequent treatment with [177Lu]Lu-DOTA-TATE. The biological relevance of this approach will be assessed through tumour response. ASTX727 is an oral fixed-dose combination of 100 mg cedazuridine and 35 mg decitabine. Cedazuridine inhibits cytidine deaminase increasing the oral bioavailability and half-life of decitabine. The key dose-limiting toxicities with demethylating agents is myelosuppression and there is concern that this may be additive with the use of [177Lu]Lu-DOTA-TATE in a safe manner.23 The recommended dosing for ASTX727 100/35 mg daily for 5 days 28 days. In this trial, patients will receive treatment for 5 days every 2 months and with this regimen we would not anticipate the degree of side-effects reported in the phase I/II studies. However, myelotoxicity will be evaluated prior to and following each cycle of ASTX727. Our preclinical combination studies lend support to this regimen being safe in a clinical setting.

LANtana proposes to explore this concept in a phase I clinical trial. If successful, LANtana will represent a step change in the management of NENs, allowing patients who currently cannot be treated with [177Lu]Lu-DOTA-TATE access to an effective treatment regimen. This trial will also be the first to show the novel manipulation of receptor expression through the use of demethylation agents which may be applicable to other tumour types as illustrated by others preclinically.

Methods and analysis

Trial objectives

Primary objective

The primary objective is to evaluate the uptake of [68Ga]Ga-DOTA-peptide-PET/CT in patients with NENs who have no or low tumorous uptake on baseline [68Ga]Ga-DOTATATE-PET/CT imaging, as a mean to image changes in epigenetic regulation of SSTR2 in response to ASTX727

Secondary objectives

Objective response rate (ORR) to combination treatment will be assessed according to RECIST 1.1. The incidence of adverse and serious adverse events (AEs/SAEs) overall and by severity, graded by the National Cancer Institute—Common Toxicity Criteria (NCI-CTC V.5.0) will be assessed. Other secondary objectives are to determine progression-free survival (PFS) defined as the time from the date of treatment to the date of the first documentation of disease progression as determined by RECIST V.1.1 or death, and to assess QoL using the European Organisation for Research and Treatment of Cancer (EORTC) questionnaires QLQ-C30 and QLQ-GI.NET21 at baseline and every 2 months until disease progression. Several translational endpoints will be explored. Expression of SSTR2 on baseline tumour biopsies, as determined by immunohistochemistry, will be correlated with baseline PET uptake parameters (SUVmax). Promoter methylation status of the SSTR2 locus in the tumour will be correlated with baseline PET uptake parameters (SUVmax), and any changes in the methylation status will be determined on repeat biopsy following 1 cycle of ASTX727 in the first five recruited patients. Changes in methylation will be correlated with changes in PET uptake. Bloods will be taken at baseline, prior to and following each cycle of ASTX7272. Changes in LINE-1 methylation status in peripheral blood mononuclear cells (PBMC) will be correlated with changes in PET uptake parameters (SUVmax), and treatment response.

Trial design

LANtana is a phase Ib study that will investigate a demethylating strategy to re-express tumorous SSTR2 as visualised on [68Ga]Ga-DOTA-TATE-PET/CT in patients with NENs that have no or low uptake on [68Ga]Ga-DOTA-TATE-PET who would otherwise be unsuitable for PRRT.

Eligibility criteria

Participants will be recruited from Hammersmith Hospital, Imperial College Healthcare NHS Trust. Patients will be provided with a verbal and written explanation of the trial and given the opportunity to discuss all aspects of the trial with both the clinician and the research nurse (online supplemental file 1). Patients who provide written consent to participate in the trial after at least 24 hours of consideration will be registered and therefore eligible to proceed to further assessments.

To be eligible patients will have a confirmed histologic diagnosis of well differentiated grade 1–3 NENs (Ki67<55%) with tumour uptake less than background liver (modified Krenning score≤2, table 1)24 on [68Ga]Ga-DOTA-TATE-PET at the majority of sites. NEN of any site will be included in the study.25 26 Patients must have progressed through at least one line of therapy. Detailed inclusion and exclusion criteria are outlined in table 2. A total of 27 patients will be recruited. Recruitment to the study commenced November 2022 and will be completed by December 2024.

Table 1

Modified Krenning Score

Table 2

Inclusion and exclusion criteria

Study procedures

Patients will undergo baseline standard of care imaging with [68Ga]Ga-DOTA-TATE-PET/CT, contrast CT scan of the chest, abdomen and pelvis, or contrast MRI. Archival tumour biopsies will be obtained or pretreatment biopsy undertaken under ultrasound guidance. Patients will then receive cycle 1 ASTX727 days 1–5 (fixed dose 35 mg decitabine+100 mg cedazuridine), orally. [68Ga]Ga-DOTA-TATE-PET/CT will be repeated post cycle 1, day 8+2 to assess SSTR2 re-expression. In a subset of patients (n=5), repeat tumour biopsy will be undertaken following 5 days of ASTX727 to assess the impact of the demethylating agent on SSTR2 gene promoter methylation within the tumour. If there is a significant change (defined below) in tumour uptake on repeat [68Ga]Ga-DOTA-TATE-PET/CT, patients will receive cycle 2 of ASTX727 after 28 days, days 1–5 followed by cycle 1 of [177Lu]Lu-DOTA-TATE, day 8+2. Combination treatment will be continued for a total of four cycles administered every 2 months. If there is not a significant change in uptake on repeat [68Ga]Ga-DOTA-TATE-PET/CT, the patient will be withdrawn from the study. Necessary levels of SSTR expression on [68Ga]Ga-DOTA-TATE-PET/CT have not been clearly defined, but lesion uptake should exceed background hepatic uptake.14 Study procedures are outlined in figure 1. To proceed with [177Lu]Lu-DOTA-TATE, uptake on [68Ga]Ga-DOTA-TATE-PET/CT should be visually greater than that of background liver in most (>50%) of lesions using the modified Krenning score (Krenning score≥3).27

Figure 1

Trial schema. 1CT chest, abdomen and pelvis or MRI (physician’s choice), routine bloods, history, physical examination, AE data collected - baseline and every 3 months until disease progression

Patients will be reviewed at baseline, prior to each cycle of ASTX727 and on a 3 monthly basis until disease progression, withdrawal from the trial or death (whichever comes first). Blood tests will be performed at the start of each cycle of ASTX727 for safety and research. Repeat research bloods will be taken at day 8 of each ASTX727 cycle. Tumour imaging will be repeated after 2 and 4 cycles of combination therapy and then 3 monthly until tumour progression. The same method used for assessment at baseline must then be used at all subsequent time points. RECIST V.1.1 criteria will be used to determine patient response to treatment, PFS and ORR. Participants will be assessed every 3 months thereafter for disease assessment and survival. QoL questionnaires (EORTC QLQ-C30 and EORTC QLQ-GINET21) will be completed at baseline, prior to each cycle of combination therapy and 3 monthly post [177Lu]Lu-DOTA-TATE until tumour progression.


ASTX727 is a fixed dose combination of 35 mg decitabine+100 mg cedazuridine and no dose reductions will be possible in the event of toxicity. However, changes in dose duration are permitted in the advent of grade 3 related AEs. Dose duration can be reduced to 4 days and 3 days as clinically indicated. Treatment delays are permitted for up to 28 days following which patients will be withdrawn from the study.

Dose modifications of [177Lu]Lu-DOTA-TATE are permitted (table 3) and dose delays up to 16 consecutive weeks is permitted for resolution of toxicities. If the drug-related toxicity resolves within 16 weeks, the subsequent cycle will be administered with a 50% dose reduction. If no further dose modifying toxicities occur, full dose can be administered on the subsequent cycle, otherwise patients will be withdrawn from the study.

Table 3

Dose modifications for [177Lu]Lu-DOTA-TATE

All AEs will be reported in a timely fashion. AEs whether expected or not, will be collected and recorded during clinical assessments prior to each ASTX cycle and 3 monthly thereafter as well as at the time of progression. WhenSAEs occur, an SAE form will be completed within 24 hours. The Chief Investigator will determine whether SAEs were ‘related’ (resulting from administration of the study treatment or procedure) or ‘unexpected’ (an event not listed in the study protocol as an anticipated occurrence) and report this to the Research Ethics Committee. Any questions concerning AE reporting will be directed to the Chief Investigator in the first instance. The Chief Investigator will notify the sponsor of all SAEs that occur. Potential related SAEs are included in online supplemental data.

Data collection

Data will be collected using paper case report forms and entered into a validated trial database by the Cancer Research Team, Imperial College NHS Healthcare Trust, where data quality will be monitored. Automatic and manual validation of entered data will be conducted. Data items relating to the safety and rights of individual participants will be dealt with as a priority. Missing data will be chased until it is either received or confirmed as not available at the trial analysis stage. For the primary analysis, there will be no data imputation for missing data in the primary end point.

Statistical analysis

The Simon’s minimax two-stage design of phase II clinical trials will be used. The primary endpoint of clinical benefit rate (CBR) in this trial is defined as percentage of patients with 20% increase in SUVmax on[68Ga]Ga-DOTA-TATE-PET/CT at days 8–10 compared with baseline. Assuming maximum unacceptable CBR (p0) for the experimental treatment of 5% and minimal acceptable CBR (p1) of at least 20%, the trial will need to recruit 27 patients in total with an interim data review after 13 patients to have 80% power and one-sided type-I error of 0.05. In the interim review, at least 1 patient with response (defined below) to ASTX727 among the first 13 patients should have been observed to move the second stage, where 14 additional patients will be accrued for a total of 27. If the trial passes interim analysis, at least 4 patients with response among the total 27 patients will have to be reported to claim the treatment effective.

Planned analyses

Image analysis. Image analysis shall be performed by two experienced PET readers in consensus (TB and MN). Visual analysis using the modified Krenning score will be performed (table 1). Quantitative analysis with lesion SUVmax and tumour to liver ratio (T/L, SUVmax tumour/SUVmean liver) will be determined (tumour SUVmax/liver SUVmean) using a 2–3 cm volume of interest in normal background liver.27 Changes in [68Ga]Ga-DOTA-TATE-PET/CT uptake parameters (SUVmax and T/L) will be calculated on a per-lesion basis. A maximum of 5 lesions per organ or at least 50% of lesions per organ, whichever is greater, will be used for quantitative analysis.

Post treatment response assessments. Response will be assessed by CT imaging (RECIST V.1.1). CR, PR and SD (response) will be collapsed into a response category while PD, missing and non-evaluable cases will be deemed to be non-response. Any changes in PBMC DNA methylation will be assessed across each cycle of therapy and associations with with tumour response explored using non-parametric tests. Scores from the EORTC QLQ-C30 and GI.NET 2 questionnaires will be analysed in accordance with EORTC guidelines.28 Kaplan-Meier and Cox regression test will be used for survival analyses.

Patient and public involvement

The design and protocol was developed with patient and public input (PPI). All patient facing materials were reviewed by PPI representatives. As part of the trial conduct PPI representatives will be part of the trial steering committee where they will provide a unique perspective on the trial conduct and reporting of endpoints.

Ethics and dissemination

This study has Clinical Trials Authorisation from the UK Competent Authority; MHRA. The Study Coordination Centre has obtained approval from the Leeds West Research Ethics Committee and Health Regulator Authority (IRAS 237279). The study has received confirmation of capacity and capability from Imperial College NHS Healthcare Trust. The study will be conducted in accordance with the recommendations for physicians involved in research on human subjects adopted by the 18th World Medical Assembly, Helsinki 1964 and later revisions. Participants’ identification data will be required for the registration process. The Study Coordination Centre will preserve the confidentiality of participants taking part in the study and is registered under the Data Protection Act. Full results will be published once recruitment is complete. The Standard Protocol Items: Recommendations for Interventional Trials reporting guidelines were used for this study.29

Ethics statements

Patient consent for publication


Infrastructural support for this research was provided by the Cancer Research UK Imperial Centre, the Imperial College Experimental Cancer Medicine Centre (ECMC), the NIHR Imperial Biomedical Research Centre (BRC) and NIHR Imperial Clinical Research Facility. The views expressed are those of the authors and not necessarily those of the NIHR or the Department of Health and Social Care.


Supplementary materials

  • Supplementary Data

    This web only file has been produced by the BMJ Publishing Group from an electronic file supplied by the author(s) and has not been edited for content.


  • Contributors RM: study doctor; contribution to manuscript. GC: contribution to manuscript MM: project manager; coordination of trial set-up, contribution to protocol and manuscript. CW: project manager; coordination of trial set-up, contribution to protocol and manuscript. SRK: coinvestigator, contribution to manuscript. MN: coinvestigator, contribution to manuscript. TB: coinvestigator; contribution to protocol, trial preparations and manuscript. EA: contribution to protocol, trial preparations and manuscript. RS: principal investigator; concept, preparation of trial, writing of protocol and manuscript. All authors read and approved the final manuscript.

  • Funding The Lantana study is sponsored by Imperial College London and funded by Advanced Accelerator Applications, a Novartis company (grant award/number: N/A). The funders also contributed drug supply and reviewed the manuscript for accuracy but had no influence on the decision to publish.

  • Competing interests RS received lecture fees from Bayer Healthcare, SIRTEX, Roche; consulting fees from SIRTEX, EISAI, Roche; received research funding (to institution) from Incyte, Boston Scientific, AAA and Astex pharmaceuticals.

  • Patient and public involvement Patients and/or the public were involved in the design, or conduct, or reporting, or dissemination plans of this research. Refer to the Methods section for further details.

  • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.