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Immediate acupuncture with GB34 for biliary colic: protocol for a randomised controlled neuroimaging trial
  1. Ning Sun1,
  2. Dong-Mei He2,
  3. Xiangyin Ye1,
  4. Lei Bin2,
  5. Yuanfang Zhou1,
  6. Xiaodong Deng1,
  7. Yuzhu Qu1,
  8. Zhengjie Li1,
  9. Shirui Cheng1,
  10. Shuai Shao2,
  11. Feng-Juan Zhao3,
  12. Tie-Huan Zhang2,
  13. Jing Cai4,
  14. Ruirui Sun1,
  15. Fan-rong Liang1
  1. 1Acupuncture and Tuina School/The 3rd Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China
  2. 2Emergency Department, The First People’s Hospital of Longquanyi District, Chengdu, Sichuan, China
  3. 3Science and Education Department, The First People’s Hospital of Longquanyi District, Chengdu, China
  4. 4Oncology-Blood Department, The First People’s Hospital of Longquanyi District, Chengdu, China
  1. Correspondence to Dr Fan-rong Liang; acuresearch{at}


Introduction As the main manifestation of gallstone disease, biliary colic (BC) is an episodic attack that brings patients severe pain in the right upper abdominal quadrant. Although acupuncture has been documented with significance to lead to pain relief, the immediate analgesia of acupuncture for BC still needs to be verified, and the underlying mechanism has yet to be covered. Therefore, this trial aims first to verify the immediate pain-alleviation characteristic of acupuncture for BC, then to explore its influence on the peripheral sensitised acupoint and central brain activity.

Methods and analysis This is a randomised controlled, paralleled clinical trial, with patients and outcome assessors blinded. Seventy-two patients with gallbladder stone disease presenting with BC will be randomised into a verum acupuncture group and the sham acupuncture group. Both groups will receive one session of immediate acupuncture treatment. Improvements in patients’ BC will be evaluated by the Numeric Rating Scale, and the pain threshold of acupoints will also be detected before and after treatment. During treatment, brain neural activity will be monitored with functional near-infrared spectroscopy (fNIRS), and the needle sensation will be rated. Clinical and fNIRS data will be analysed, respectively, to validate the acupuncture effect, and correlation analysis will be conducted to investigate the relationship between pain relief and peripheral–cerebral functional changes.

Ethics and dissemination This trial has been approved by the institutional review boards and ethics committees of the First Teaching Hospital of Chengdu University of Traditional Chinese Medicine, with the ethical approval identifier 2019 KL-029, and the institutional review boards and ethics committees of the First People’s Hospital of Longquanyi District, with the ethical approval identifier AF-KY-2020071. The results of this trial will be disseminated through peer-reviewed publications and conference abstracts or posters.

Trial registration number CTR2000034432.

  • complementary medicine
  • pain management
  • hepatobiliary disease

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Strengths and limitations of this study

  • This randomised controlled trial will first use functional near-infrared spectroscopy (fNIRS) to investigate cerebral changes during acupuncture for biliary colic (BC).

  • Combining observations of both peripheral acupoint sensitisation and cerebral activity influenced by acupuncture will contribute to exploring the partial mechanism of acupuncture for BC alleviation.

  • Both objective and subjective outcomes will be used to evaluate the effects of acupuncture treatment on BC.

  • Owing to a limited number of fNIRS measurement channels, we will restrict our analysis to the dorsolateral prefrontal cortex and primary somatosensory cortex of the patients.

  • The cumulative effect of acupuncture for patients with gallstone disease with recurrent pain and accompanying discomforts, such as nausea and vomiting, will be further studied in the future.


Gallstone disease is one of the most common gastrointestinal and biliary system disorders.1 As the main manifestation of gallstone disease, biliary colic (BC) is severe pain in the right upper abdominal quadrant that attacks patients for at least 20–30 min.2–4 In the developed world, 10%–15% of adults have gallstones, and BC occurs in 1%–4% of those with gallstones each year.5 It is reported that the cumulative probability of developing BC in initially asymptomatic people is approximately 11.9%±3.0% at 2 years and 25.8%±4.6% at 10 years.6 Moreover, BC can significantly impair sleep, mood and quality of life and cause major economic and social burdens.7 8

Compelling evidence indicates that non-steroidal anti-inflammatory drugs (NSAIDs) are effective in relieving BC.9 However, long-term use of these medications has been considered to carry side effects.10–12 As one of the complementary therapies, acupuncture is thought to be effective for alleviating acute pain with rare side effects.13–15 For many diseases with visceral pain, acupuncture has been proven to be effective. For instance, Zhao et al demonstrated that acupuncture is beneficial for the alleviation of angina as an adjunctive therapy.16 More recently, Pei et al reported that acupuncture may be more effective than conventional drugs for abdominal pain relief of patients with irritable bowel syndrome.17 However, although several studies have shown that acupuncture can relieve BC,18–21 the immediate analgesia of acupuncture for BC still needs to be verified.

In the peripheral system, acupuncture can inhibit the contractions of Oddi’s sphincter, bidirectionally regulate the gallbladder pressure, promote the secretion of hepatic bile and so on.22–25 However, the central mechanism underlying acupuncture for BC relief still needs further investigation. Brain activity is closely correlated with the regulation of visceral function, and central integration is the key to achieving good regulation of visceral function by acupuncture.26 27 At the same time, the state of acupoints and the function of the viscera are considered to be dynamic, having a two-way relationship with one another.28

The rapid development of neuroimaging technologies, such as functional MRI (fMRI), positron emission tomography, electroencephalography, functional near-infrared spectroscopy (fNIRS), has provided possibilities for neural activity research. Among them, fMRI is the most commonly used technique. However, in the current study, it is difficult for patients with BC to remain confined during MRI, with their body and head motions strictly controlled.29 Compared with fMRI, fNIRS is similarly successful in detecting brain functional activities by measuring concentration changes of oxygenated and deoxygenated haemoglobin (△HbO and △HbR), but it is more flexible without these limitations and has a better temporal resolution.30 In addition, fNIRS is also a non-invasive method and has better spatial resolution than electroencephalography.31 This neuroimaging technique has been used to assess cortical activity in diverse diseases, such as pain disease,32 attention-deficit/hyperactivity disorder,33 cerebral palsy34 and cognitive impairment.35

The current study aims to (1) retestify the immediate analgesic effect of acupuncture by comparing it to sham acupuncture; (2) investigate the potential central mechanism of this analgesic effect by using fNIRS to monitor patients’ brain activity during acupuncture procedure; (3) explore the underlying peripheral–cerebral influence by correlating the clinical improvement and pain threshold of acupoint with cerebral neural activity.


Study design

This is a 1:1 randomised placebo-controlled trial with two parallel groups. This trial will be reported in accordance with the Standard Protocol Items: Recommendations for Intervention Trials guidelines36 and will follow the principles of the Consolidated Standards of Reporting Trials and Standards for Reporting Interventions in Clinical Trials of Acupuncture statements.37 38

Seventy-two participants will be recruited in this study, with 36 participants allotted to each group. All participants will receive one session of acupuncture treatment. As shown in figures 1 and 2, clinical measurements and behavioural data of all participants will be assessed before and after their acupuncture treatment. Each patient’s cerebral activity will be synchronously detected by fNIRS during acupuncture treatment.

Figure 1

Flow chart of the study design. BC, biliary colic; fNIRS, functional near-infrared spectroscopy.

Figure 2

Standard protocol items. fNIRS, functional near-infrared spectroscopy; NRS, Numeric Rating Scale; SAS, Zung Self-rating Anxiety Scale; SDS, Zung Self-rating Depression Scale; SF-12, 12-item Short-form Health Survey.

Patient population and recruitment strategy

Participants will be screened and recruited from the emergency department and the general surgery department of the First Teaching Hospital of Chengdu University of Traditional Chinese Medicine (TCM) (Chengdu, China) and the First People’s Hospital of Longquanyi District (Chengdu, China) from July 2020 to December 2021. Trained researchers will conduct screening of participants. All patients will be fully informed of the experimental procedures, and informed consent will be obtained from all participants prior to their enrolment. Participants will be informed that they are free to withdraw from the study without penalty at any time. For this study, the diagnosis of gallbladder stone disease (GSD) follows the criteria of the European Association for the Study of the Liver.39 Patients with GSD who manifest BC and match the inclusion criteria will be included.

Inclusion criteria

The study inclusion criteria are as follows:

  1. Age between 18 and 60 years.

  2. Right handed.

  3. Mild tenderness in the right upper abdomen, with Murphy’s sign (−).

  4. No history of having taken any NSAIDs within 2 weeks before enrolment.

  5. Signed the informed consent form.

Exclusion criteria

The study exclusion criteria are as follows:

  1. Calculus in the bile duct.

  2. Acute cholecystitis, acute suppurative, gangrenous, incarcerated cholecystitis, or perforation of the gallbladder with diffuse peritonitis.

  3. Serious complications or primary diseases affecting the cerebral, cardiovascular, liver, kidney, endocrine or haematopoietic system.

  4. Severe digestive system diseases, such as peptic ulcer, upper gastrointestinal haemorrhage, gastric tumour, Crohn’s disease or irritable bowel syndrome.

  5. Other causes of upper abdominal pain, such as gastritis, enteritis, pancreatitis or peritonitis.

  6. White cell count of greater than 15×109/L with a systemic inflammatory response.

  7. Mental disorders, such as serious anxiety and/or depression (at least 70 points on the Zung Self-Rating Anxiety Scale or at least 72 points on the Zung Self-rating Depression Scale).40 41

  8. Taking other medications, such as chronic analgesic drugs.

  9. Pregnancy or lactating women.

  10. History of participation in any other clinical trial in the past 3 months.

  11. History of receiving any acupuncture treatment in the past month.

Sample size considerations

According to a previous study, a Visual Analog Scale score of acute pain decreased by 2.4 points after acupuncture and by 0.7 points after sham acupuncture.42 In this study, we anticipate a reduction in the Numeric Rating Scale (NRS) score by 2.4 points after acupuncture and a reduction by one point after sham acupuncture. With α=0.05, 1 − β=0.8 and a SD of 2, 33 participants would be required per group. Estimating a dropout rate of 10%, we planned to enrol 72 participants, with 36 allotted to each group. Based on previous neuroimaging studies, the optimal sample size for a study is between 16 and 32 subjects.43 Considering the dropout rate and loss of data due to head motion, 25 participants per group will undergo fNIRS imaging in this trial.

Randomisation and blinding

A random sequence will be generated by a third-party professional statistician using the SAS V.9.2 software (SAS Institute). The randomisation list will be placed in sealed opaque envelopes and opened by a non-involved investigator in consecutive order. A total of 72 participants with GSD will be randomised in a ratio of 1:1 to the acupuncture with Yanglingquan (GB34) group or the sham acupuncture group, with 36 patients in each group. It is difficult to blind the acupuncturists to treatment allocation, but the patients, outcome assessors, and statisticians will be blinded to treatment allocation. Participants in different groups will be separated into cubicles to limit communication, and the same type of Park device will be used for ensuring the blinding of participants. Moreover, participants will be told that there are two different types of acupuncture provided in this study; terms such as “placebo” or “sham” will not be used. Therefore, sham acupuncture aims to control not only the superficial and cutaneous somatosensory effects around the GB34 acupoint but also the cognitive processing induced by the participants’ expectations of acupuncture.44

Patient and public involvement

Patients in this trial will not be involved in the design, recruitment or conduct of the study. The patients will be able to view the study results via social media.


Participants will receive one session of acupuncture either on the right GB34 or on the sham acupoint, which will be conducted by trained, licensed acupuncturists with at least 3 years of clinical experience in acupuncture. The sham acupoint is on the lateral side of the tibia, 1 to 2 cm lateral to ST36 (Zusanli)45 (figure 3). The location of GB34 is on the lateral side of the lower leg, in the depression anterior and inferior to the head of the fibula. The location of ST36 is four fingers’ breadth below the lower margin of the patella and one finger’s breadth laterally from the anterior crest of the tibia.

Figure 3

Location of acupoint or non-acupoint in the trial.

Both groups will undergo the same acupuncture procedure and manipulation. For the acupuncture procedure, four different acupuncture manipulations will be used. Specifically, the first task is needle insertion (NI), followed by an initial twirl to activate the acupoint. The second task is the first run of needle twirl, which is repeated four times (T1, T2, T3, and T4) to keep the acupoint activated. The duration of the second task is 140 s, including four twirls (4×20 s) and three rest times (3×20 s). Then, the needle remains for 5 min to maintain deqi sensation. The third task is the second run of needle twirl, which is repeated four times (T5, T6, T7, and T8) to strengthen the deqi sensation. The third task is the same length of time as the second task (140 s). Finally, the last task is needle removal (NR). The acupuncture procedure will last a total of 10 min and 30 s (figure 4).

Figure 4

Experimental paradigm.

For acupuncture manipulation, disposable sterile filiform needles (0.25×40 mm; Huatuo, Suzhou, China) with a Park device will be used in the acupuncture group to be perpendicularly inserted into the point at a depth of 21–26 mm after skin disinfection using alcohol (figure 5). During each needle twirl, the needle will be bidirectionally twisted within 90° to 180°, lifted, and thrust at an even amplitude between 3 and 5 mm for 1 to 1.5 Hz (60–90 times/min) to induce and maintain the deqi sensation (including soreness, numbness, distention, and heaviness).46 47

Figure 5

Verum acupuncture and sham acupuncture.

The sham acupuncture group will receive non-insertive acupuncture using the Park sham needle supported by the Park device (figure 5).48 The acupuncturist will be instructed to lightly position the sham needle against the surface of the skin to minimise any physiological effect.49

Acupuncture will be discontinued and patients will be treated immediately when they experience severe discomforts, such as pain, bleeding or fainting. After finishing the acupuncture treatment, participants will be able to receive medication if they still have BC, and the type and dosage of medication used will also be recorded in the case report form (CRF).

Outcome measures

Clinical outcome assessments

The primary outcome is the mean difference in the change in pain intensity between before and after an intervention. To measure patients’ self-reported pain intensity before and after acupuncture,50 51 the NRS will be used. The NRS is a horizontal line with 10 segments labelled from 0 (‘no pain at all’) to 10 (‘the worst pain imaginable’).

Other clinical data, including demographic information, participants’ quality of life, and psychosocial state at the baseline, will also be recorded. The standard protocol items of the study are exhibited in figure 2. The 12-item Short-form Health Survey,52 Zung Self-rating Anxiety Scale40 and Zung Self-rating Depression Scale41 will be used to evaluate participants’ quality of life and psychosocial state at baseline.

To assess deqi during acupuncture, a needle-sensation evaluation form will be deployed to two groups at four time points: after NI, after T4, after T8, and after NR. This form is based on the Chinese version of the modified Massachusetts General Hospital Acupuncture Sensation Scale (C-MASS), and its validity and reliability have been previously examined.53 54 Needle sensation is an indispensable part of acupuncture treatment.55 The needle-sensation evaluation form includes deqi features (soreness, distention, numbness, heaviness, dull pain, and so on) and a rating of deqi intensity. Patients will be asked to describe the intensity of deqi with a 10-point Visual Analog Scale labelled from 0 (‘none’) to 10 (‘unbearable’).

Pain threshold measurements of the acupoint

Based on the TCM theory, a disorder of the viscera inside the body must have some manifestation outside, and the acupoint can show a person’s response to disease externally. To explore the activation of the (sham) acupoint and its association with pain relief among patients with BC after acupuncture, the mechanical and pressure pain thresholds (PPTs) will be measured.

The mechanical pain threshold will be measured using the electronic Von Frey esthesiometer (model 2390; IITC Life Science, Woodland Hills, California, USA). It has been found that this device can detect hyperalgesia and changes in sensory nerve function.56–58 At the point, a progressive pressure will be applied at a rate of 10 g/s. The average mechanical pain threshold for the GB34 point/sham acupoint will be calculated.

The PPT will be measured using the FDIX force gauge (Force One FDIX; Wagner Instruments, Greenwich, Connecticut, USA). PPT is widely used in clinical practice as a semiobjective method with which to quantify localised pain.59 60 At the point, a progressive pressure will be applied at a rate of 100 gf/s. The average PPT for the GB34 point/sham acupoint will be calculated.

If there is a difference of more than 15 g/500 gf (separately for the mechanical pain threshold and PPT) between the two measurements performed at one acupoint, a third group of measurements will be taken. The two devices described have been used in our previous study.61

fNIRS imaging and data collection fNIRS—a method of optical neuroimaging that characterises haemodynamic changes at the brain’s surface in a priori–identified regions of the brain—will be used for the measurement of cortical neural activation of patients’ brains. An fNIRS system (OMM-3000; Shimadzu, Kyoto, Japan) that has continuous-wave laser diodes with 780, 805 and 830 nm wavelengths will be used in this experiment.62 The optodes will be positioned over the international 10–20 system for electroencephalography electrode placement by using individually sized caps selected according to head circumference. About 10 locations will be spatially adjusted across all cap sizes to maintain consistent coverage of our regions of interest.63 64 For accurate fixation, the optodes will be mounted on the holder in the NIRS cap, and the distance between pairs of light sources and detector optodes will be set to 2.5–3 cm.65

In the present study, eight emitters and eight detectors will be used to thoroughly investigate the dorsolateral prefrontal cortex (DLPFC) and primary somatosensory cortex of the human brain. The emitters will be positioned by considering Fpz and Cz as reference points. A total of 20 channels will be configured using emitter–detector combinations.

Safety and monitoring

To provide an overview of their health status, each participant in this study will undergo a series of health evaluations, including an abdominal ultrasound, blood biochemical test and ECG examination. Any acupuncture treatment-related adverse events, such as haematoma, subcutaneous haemorrhage, serious pain, local infection, broken needle and fatigue, will be processed immediately and recorded in detail in the CRF. Adverse events of the acupuncture group and sham acupuncture group will be compared.

Quality control and data collection and management

All researchers will receive a series of training before the start of this study, to ensure that they fully understand the study protocol and standard procedures. CRFs labelled by unique numeric identifiers will be used to collect relevant data, adverse events and safety assessments for patients. Only outcome assessors have access to CRFs and will perform the double data entry. The Evidence-based Medicine Center of the Chengdu University of TCM will be in charge of supervising the study and monitoring data every 3 months.

Statistical analysis

Clinical data analysis

Clinical data will be statistically analysed using the Statistical Package for the Social Sciences V.22.0 software program (IBM Corporation), with a significance level of 0.05, and all hypothesis tests will be two tailed. Skewed data distribution will be normalised. Means, SD and 95% CIs will be computed to describe continuous variables.

Baseline demographic characteristics, NRS scores and pain threshold values between the two groups will be analysed with the χ2 test or Fisher’s exact test for categorical measures and with the t-test or Wilcoxon rank-sum test for continuous measures. The needle sensation will be compared between the two groups at four time points by using a 2×4 repeated-measures analysis of covariance approach.

fNIRS data analysis

Data will be analysed in MATLAB V.8.6 (Mathworks, Natick, Massachusetts, USA). First, a wavelet motion-correction procedure will be used for motion artefacts with all-optical density data.64 Next, the optical density data will be converted to HbO and HbR values using the modified Beer-Lambert law.66 Data from individual channels will be visually inspected for excessive noise. Channels with excessive noise will be excluded from subsequent analyses. In addition, we will employ a wavelet-based method to remove global noise, which is used as a powerful mathematical tool in fNIRS data preprocessing.67–69 To obtain an average response for each of the 20 channels in each participant, the haemodynamic response function will be estimated by a general linear model approach using ordinary least squares.

Correlation analyses will be used to investigate associations among clinical outcomes, pain threshold values of the acupoint and fNIRS data. Differences in correlations between two groups will be further examined by group-level analysis.


This is a randomised controlled, paralleled clinical trial seeking to explore the immediate pain alleviation of acupuncture for BC and its influence on the peripheral sensitised acupoint and central brain activity.

According to the TCM theory, GB34 is the Lower He-sea point of the gallbladder and is considered specifically connected to the gallbladder. As one of the most common acupoints used for GSD treatment, acupuncture with GB34 is considered effective for the treatment of gallstone disease.19 20 24 Some clinical studies have reported that sham acupuncture interventions, such as needle insertion at a sham acupoint, are not entirely ineffective due to their associations with moderately large, non-specific effects.70 71 Thus, in clinical trials, sham acupuncture involving penetrating needling is not recommended.72 73 To better verify the effectiveness of manual acupuncture with GB34, a non-inserted needle at a non-acupoint is chosen as the sham control technique to minimise physiological effects in the sham group. Thus, a non-acupoint on the lateral side of the lower leg, which is almost at the same level as the GB34 acupoint, will be chosen in the sham group to blind participants in this study. However, a recent review indicates that sham acupuncture and verum acupuncture have almost identical efficacy when the dermatomes that are stimulated highly overlap.74 The present study may help to test these authors’ arguments. Because the pain of patients with BC is so severe that they can tolerate fNIRS imaging for only a short time, acupuncture with a short-time needle retention time (10 min and 30 s) will be chosen after the pilot study.

Recent Chinese research has found that, when the body is in a state of disease, disease-related acupoints can be sensitised.75–77 Pain sensitivity is one of the most important sensitivity manifestations at sensitised acupoints, and its degree may be correlated with disease condition.57 58 Considering the importance of acupoint sensitisation for the acupuncture effect, we will measure the pain threshold of GB34 and observe the efficacy of acupuncture at GB34 for the treatment of BC. In addition to NRS and C-MASS, which offer subjective-state measurements, we also evaluated the impact of acupuncture treatment on objective outcomes using pain threshold values and the fNIRS data. Meanwhile, correlations will be analysed to investigate associations among clinical outcomes, pain threshold values of the acupoint, and fNIRS data.

fNIRS is suitable for all possible participant populations, from newborns to the elderly, and in a variety of experimental settings both inside and outside the laboratory.78 79 Some diseases with acute pain, such as BC, are difficult to investigate in tightly restrained environments, such as an fMRI system. The process of fNIRS recording is silent, relatively comfortable and does not impose physical constraints on the participant,80 which means it is a good choice of imaging modality for many studies of patients with acute pain. Moreover, a major reason for choosing fNIRS is that the usage of metal objects is applicable. For acupuncture studies, because acupuncture needles are magnetic metals, it is impossible to implement treatment and fMRI imaging at the same time. Therefore, using fNIRS, the synchronisation of acupuncture treatment and brain-image collection can be realised.

According to previous literature, acupuncture stimulation modulates the cortical activities of the somatomotor area in humans.81 82 Moreover, acupuncture at GB34 can activate the somatomotor area and DLPFC in healthy subjects in our pilot trial. The DLPFC has displayed a critical role in pain modulation.83 In particular, DLPFC is a key node of several brain networks and may be a key node of networks implicated in nociceptive processing and control.84 A study reported that left DLPFC activity is negatively correlated with pain unpleasantness.85 Freund et al found that subjects given instructions to suppress pain show increased activation of the bilateral DLPFC during prolonged acute pain stimulation.86 There is converging evidence that the DLPFC has a role in cognitive components of the pain experience. Therefore, the somatomotor area and DLPFC will be chosen as brain regions of interest for this study.

In this trial, the following measures will be taken to strengthen quality control: (1) this study restricts participants to ages between 18 and 60 years and to being right handed, avoiding the impact of age and handedness on brain function and structure; (2) to get stable statistic power, 36 patients will be included in each group for clinical evaluation and 25 patients in each group for the central mechanism study; (3) all researchers will be uniformly trained to understand the design and process of the trial, and the acupuncture treatment will be performed by two licensed acupuncturists, who have received special and standardised training before the trial; (4) one technician will perform all scans in the same fNIRS machine according to the operation standard and the design of the study in the fixed condition, and, before being scanned, the mental state of each participant will be evaluated using the Zung Self-rating Anxiety Scale and Zung Self-rating Depression Scale.

In summary, this study is planned as a randomised controlled, paralleled clinical trial to evaluate the efficacy and central mechanism of acupuncture treatment on BC compared with sham acupuncture. To our knowledge, it is the first functional brain imaging study focusing on exploring the immediate pain alleviation of acupuncture for BC and its influence on the peripheral sensitised acupoint and central brain activity. The findings may provide evidence of the clinical benefits and mechanisms of acupuncture for patients with BC.

Ethics statements

Patient consent for publication

Ethics approval

This study was approved by Sichuan Regional Ethics Review Committee of Traditional Chinese Medicine (2019KL-029); Medical Ethics Committee of Hospital Affiliated to Chengdu University of Traditional Chinese Medicine (AF-KY-2020071). Participants gave informed consent to participate in the study before taking part.



  • Contributors RS, F-rL and NS participated in the conception and design of the trial. NS, D-MH and XY participated in drafting the manuscript. LB, XD, YZ, T-HZ and SS will be responsible for participants recruitment. NS and YZ will perform the acupuncture for participants. ZL, SC and JC will be in charge of data collection. NS, RS and F-JZ will conduct data analysis. All authors discussed, read, revised the manuscript and all approved the publication of this protocol.

  • Competing interests None declared.

  • Patient and public involvement Patients and/or the public were not involved in the design, or conduct, or reporting, or dissemination plans of this research.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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