Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Validation of an Automated Enzyme Immunoassay for Interleukin-6 for Routine Clinical Use

  • Peter Fraunberger , Matthias Pfeiffer , Peter Cremer , Ernst Holler , Dorothea Nagel , Isabelle Dehart , Michael Thein , Autar K. Walli and Dietrich Seidel

Abstract

Serum levels of Interleukin-6 (IL-6), a proinflammatory cytokine, are increased in early stages of inflammatory diseases such as infection and sepsis. Assay systems which permit its measurement within a few hours and as a single measurement have not been reported so far. We therefore evaluated a now commercially available automated method for IL-6 measurement on the Cobas Core® immunological analyzer (Roche Diagnostic Systems) which enables single IL-6 measurement within about 1 hour. The automated assay correlates well with an established, manual microtiter plate assay (Biosource GmbH) which uses the same antibodies and reagents (r=0.98). Accuracy of the automated method was established by adding known amounts of IL-6 international reference preparation. Recovery of the international standard was in the range of 92–104 %. The automated assay had a precision of singletons below 6% and was linear up to 2800 pg/ml. This automated assay provides a suitable, convenient and time saving method for measurement of IL-6 serum levels in the routine clinical laboratory.

:
Published Online: 2005-06-01
Published in Print: 1998-10-13

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

Downloaded on 29.3.2024 from https://www.degruyter.com/document/doi/10.1515/CCLM.1998.141/html
Scroll to top button