Bile acids promote glucagon-like peptide-1 secretion through TGR5 in a murine enteroendocrine cell line STC-1
Section snippets
Materials and methods
Materials. Lithocholic acid and deoxycholic acid were from Nacalai Tesque. Linolenic acid, 8-bromo-cAMP, and MDL12330A were obtained from Sigma. Forskolin and Cholera toxin were purchased form Calbiochem.
Cell culture. Murine enteroendocrine cell line STC-1 [10] was maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 15% (v/v) horse serum (HS) and 5% (v/v) fetal bovine serum (FBS).
Real-time PCR analysis. Total RNA was isolated using Isogen (Nippon Gene) and subjected to polymerase
Bile acids promote GLP-1 secretion in a murine enteroendocrine cell line STC-1 through TGR5
Recently, we showed that unsaturated free fatty acid, such as linolenic acid, promoted GLP-1 secretion in murine intestinal STC-1 cells [11]. To date, however, it has not been reported that bile acids promote GLP-1 release in intestinal cultured cells. In the present study, we examined whether bile acids promote GLP-1 secretion in STC-1 cells. As shown in Fig. 1, we first confirmed that linolenic acid-induced the release of GLP-1 in STC-1 cells. Also, we found that two bile acids, lithocholic
Acknowledgments
This study was performed through Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government. S.K. is supported by the 21st Century COE Program “Knowledge Information Infrastructure for Genome Science.”
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