Reproducibility of Retinal Thickness Measurements in Healthy Subjects Using Spectralis Optical Coherence Tomography

doi:10.1016/j.ajo.2008.09.005

American Journal of Ophthalmology

Volume 147, Issue 3, March 2009, Pages 467-472

https://doi.org/10.1016/j.ajo.2008.09.005 Get rights and content

Purpose

To test the reproducibility of retinal thickness measurements in healthy volunteers of a new Frequency-domain optical coherence tomography (OCT) device (Spectralis OCT; Heidelberg Engineering, Heidelberg, Germany).

Design

Prospective, observational study.

Methods

Forty-one eyes of 41 healthy subjects were included into the study. Intraobserver reproducibility was tested with 20 × 15 degree raster scans consisting of 37 high-resolution line scans that were repeated three times by one examiner (M.N.M.). Mean retinal thickness was calculated for nine areas corresponding to the Early Treatment Diabetic Retinopathy Study (ETDRS) areas. Coefficients of variation (COV) were calculated.

Results

Retinal thickness measurements were highly reproducible for all ETDRS areas. Mean total retinal thickness was 342 ± 15 μm. Mean foveal thickness was 286 ± 17 μm. COVs ranged from 0.38% to 0.86%. Lowest COV was found for the temporal outer ETDRS area (area 7; COV, 0.38%). Highest COV was found for the temporal inner ETDRS area (area 3; COV, 0.86%). Mean difference between measurement 1 and 2, measurement 1 and 3, and measurement 2 and 3 for all ETDRS areas was 1.01 μm, 0.98 μm, and 0.99 μm, respectively.

Conclusion

Spectralis OCT retinal thickness measurements in healthy volunteers showed excellent intraobserver reproducibility with virtually identical results between retinal thickness measurements performed by one operator.

Section snippets

Methods

Forty-one eyes of 41 healthy subjects (mean age, 28 ± 5 years; 24 females) were included into the study. Exclusion criteria were the presence of a refractive error of > ±5 diopters (D), astigmatism of > 2 D, media opacifications, a history of ocular trauma or ocular diseases affecting the cornea, lens, retina, or optic nerve. The intraocular pressure (IOP) of the study eye had to be between 11 mm Hg and 21 mm Hg. All subjects received a slit-lamp exam, fundus biomicroscopy, and indirect

Results

The Table shows mean retinal thickness values, SDs, and COV values for the nine ETDRS areas tested. Retinal thickness measurements were highly reproducible for all ETDRS areas. Mean total retinal thickness was 342 ± 15 μm. Mean foveal thickness was 286 ± 17 μm. Mean difference between measurement 1 and 2, measurement 1 and 3, and measurement 2 and 3 for all ETDRS areas was 1.01 μm, 0.98 μm, and 0.99 μm, respectively. Mean COV was 0.54 ± 0.13%. Lowest COV was found for the temporal outer ETDRS

Discussion

The latest commercially available generation of FD-OCT has several advantages compared with conventional TD-OCT. High-speed imaging allows increasing the number of acquired B scans to yield greater retinal coverage and high-definition 3-dimensional images. Higher scan density leads to more detailed retinal thickness maps with less need of data interpolation. In this study, 37 B scans were used to scan a 20 × 15 degree area centered on the fovea. The Spectralis OCT has the option to increase

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Original articleReproducibility of Retinal Thickness Measurements in Healthy Subjects Using Spectralis Optical Coherence Tomography

Purpose

Design

Methods

Results

Conclusion

Section snippets

Methods

Results

Discussion

Ophthalmology

Ophthalmology

Ophthalmology

Laser scanning in ophthalmology

Fortschr Ophthalmol

Optical coherence tomography

Science

Optical coherence tomography of the human retina

Arch Ophthalmol

Sensitivity advantage of swept source and Fourier-domain optical coherence tomography

Opt Express

Ultra-high resolution, high-speed, Fourier-domain optical coherence tomography and methods for dispersion compensation

Opt Express

High-speed, ultra-high resolution retinal imaging using spectral/Fourier-domain OCT

Conf Lasers Electrooptics

High-definition and three-dimensional imaging of macular pathologies with high-speed ultra-high resolution optical coherence tomography

Ophthalmology

Three-dimensional ultra-high resolution optical coherence tomography of macular diseases

Invest Ophthalmol Vis Sci

Original article
Reproducibility of Retinal Thickness Measurements in Healthy Subjects Using Spectralis Optical Coherence Tomography