ArticleDouble probing of human spermatozoa for persistent histones, surplus cytoplasm, apoptosis and DNA fragmentation
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Leyla Sati MSc is currently a PhD student in the Department of Histology and Embryology, Akdeniz University, Turkey, having previously worked as a pre-doctoral Fellow in the Sperm Physiology Laboratory, Yale University School of Medicine with Dr Gabor Huszar. Her research interests include the various biochemical markers of human sperm maturity and function, and the relationship between tyrosine phosphorylation in human spermatozoa and sperm binding to the zona pellucida and hyaluronic acid.
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2014, Reproductive BioMedicine OnlineCitation Excerpt :This study examined whether vesicles bound all spermatozoa equally or preferentially, based on the state of sperm development. To test this, spermatozoa were first allowed to bind to vesicles (monitored by red fluorescence) and then stained with either a cytoplasmic (CK) or a nuclear (aniline blue) marker specific to arrested sperm development (Sati et al., 2008). Using fluorescent CK immunocytochemistry, the sperm population could be divided into three categories (Supplementary Figure 2): normally developed (nonfluorescent), fully arrested (green fluorescent) and intermediate state.
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2012, Best Practice and Research: Clinical Obstetrics and GynaecologyThe effect of age on the expression of apoptosis biomarkers in human spermatozoa
2010, Fertility and SterilityCitation Excerpt :Spanò et al. (16) used the SCSA, which measures the susceptibility of sperm DNA to in situ acid-induced denaturation, and found that the SCSA results can be influenced by the age of the donor. Similar trends were published by other investigators (18, 47, 48). The present study also confirmed that advancing male age is associated with subtle hormonal changes, such as increased FSH and LH levels, a significant increase in FAI, as well as a decline in sperm concentration, with a similar trend observed for motility and morphology (4, 5, 13, 14).
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2023, Holistic Approach to Assisted Reproductive TechnologyRole of Sperm-Hyaluronic Acid Binding in the Evaluation and Treatment of Subfertile Men with ROS-Affected Semen
2020, Male Infertility: Contemporary Clinical Approaches, Andrology, ART and Antioxidants: Second Edition
Leyla Sati MSc is currently a PhD student in the Department of Histology and Embryology, Akdeniz University, Turkey, having previously worked as a pre-doctoral Fellow in the Sperm Physiology Laboratory, Yale University School of Medicine with Dr Gabor Huszar. Her research interests include the various biochemical markers of human sperm maturity and function, and the relationship between tyrosine phosphorylation in human spermatozoa and sperm binding to the zona pellucida and hyaluronic acid. She has been nominated for and awarded prizes at international meetings, including the American Society of Reproductive Medicine and the 2007 International Dermatology and Andrology Conference in Egypt.