An HPLC/MS/MS assay for tacrolimus in patient blood samples Correlation with results of an ELISA assay
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Cited by (47)
In vitro selection of tacrolimus binding aptamer by systematic evolution of ligands by exponential enrichment method for the development of a fluorescent aptasensor for sensitive detection of tacrolimus
2020, Journal of Pharmaceutical and Biomedical AnalysisCitation Excerpt :So far, various approaches have been reported for the quantitative detection of TAC [5], such as enzyme-linked immunosorbent assay (ELISA) [7–11] in which the mechanism of detection is based on the recognition of the metabolites of target instead of real target, itself. However, analytical approaches, such as HPLC-MS and HPLC-MS/MS are based on real targets detection rather than their metabolites [12,13]. Although these approaches are appropriate for TDM but they are time-consuming and need expensive equipment and skilled person.
Application of volumetric absorptive microsampling device for quantification of tacrolimus in human blood as a model drug of high blood cell partition
2017, Journal of Pharmaceutical and Biomedical AnalysisCitation Excerpt :As typical concentration levels in therapeutic drug monitoring samples were 3–15 ng/mL [14], a sensitive assay method is required, which is a challenge using a small volume of matrix in microsampling. A number of bioanalytical methods have been reported for the determination of tacrolimus in human blood [15–17] and assay methods in DBS have been reported as well [18–20]. We have established a reproducible bioanalytical method for tacrolimus on VAMS with the lower limit of quantification (LLOQ) of 1 ng/mL using only 0.01 mL blood samples.
Therapeutic drug monitoring of tacrolimus by liquid chromatography-tandem mass spectrometry: Is it truly a routine test?
2012, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :There have been several recent reviews on the use of LC–MS/MS for the monitoring of tacrolimus and the other immunosuppressants in this class of drugs [14–17]. The early tacrolimus LC–MS/MS methods, as published by our group and others, had extensive sample preparation; with protein precipitation followed by off-line solid phase extraction or liquid-liquid extraction [18–20]. These methods were developed to encompass a wide analytical range (typically, 0.2–100 μg/L) as, at the time, knowledge of tacrolimus therapeutic ranges were still evolving.
Pharmacokinetics in stable heart transplant recipients after conversion from twice-daily to once-daily tacrolimus formulations
2011, Journal of Heart and Lung TransplantationCitation Excerpt :Whole-blood tacrolimus concentrations were determined by Covance Laboratories (Harrogate, UK; Madison, WI) using validated high-performance liquid chromatography tandem mass spectroscopy (HPLC/MS/MS) assay methods (lower limit of quantification, 0.059 ng/ml). The assay was based on the method developed by Alak et al.6 Whole-blood calibration standards, quality control samples, and study samples were thawed, and 1-ml aliquots were taken.
Determination of tacrolimus in rabbit aqueous humor by liquid chromatography-electrospray ionization tandem mass spectrometry
2008, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesSimultaneous determination of three isomeric metabolites of tacrolimus (FK506) in human whole blood and plasma using high performance liquid chromatography-tandem mass spectrometry
2006, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences