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Serum MIP-1α and IL-8 in septic patients

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Abstract

We studied blood MIP-1α and IL-8 in 38 septic patients and 5 healthy volunteers. Both chemokines were undetectable in the healthy volunteers. In sepsis, serum MIP-1α was detected in 45% of the patients and IL-8 in 84%. The levels of MIP-1α, but not of IL-8, correlated with CRP, IL-6 and TNFα levels. Complication, including various organ failures and mortality, showed no correlation with serum MIP-1α levels. In contrast, we found increased levels of serum IL-8 in septic patients with disseminated intravascular coagulation, central nervous system (CNS) dysfunction or renal failure, and the mortality rate was higher in the IL-8-detectable group than in the IL-8 undetectable group (50% vs 0%,p<0.05). In conclusion, the production of both MIP-1α and IL-8 was increased and initially detectable levels of circulating IL-8 predicted high mortality in sepsis.

Objective

To determine the significance of the C-C chemokine MIP-1α and the C-X-C chemokine IL-8 in sepsis.

Design

Prospective study.

Setting

Clinical investigation, emergency department and general intensive care unit of university hospital.

Patients and participants

38 septic patients and 5 healthy volunteers were studied. Sepsis was diagnosed following the criteria formulated by ACCP/SCCM.

Interventions

10–20 ml of blood was drawn from each patient at the time of initial diagnosis of sepsis.

Measurements and results

MIP-1α and IL-8 were determined by sand-wich ELISA. Both chemokines were undetectable in the healthy volunteers. In sepsis, serum MIP-1α was detected in 45% of the patients and IL-8 was detected in 84%. The levels of MIP-1α, but not of IL-8, correlated with CRP, IL-6 and TNFα levels. Complications, including various organ failures and mortality, showed no correlation with serum MIP-1α levels. In contrast, we found increased levels of serum IL-8 in patients with disseminated intravascular coagulation (DIC) (p<0.05), central nervous system (CNS) dysfunction (p<0.05), renal failure (p<0.01) and the mortality rates were higher in the IL-8 detectable group than in the IL-8 undetectable group (50% vs 0%,p<0.05).

Conclusions

The production of MIP-1α and IL-8 was increased in sepsis. Furthermore, an initially detectable level of circulating IL-8, but not MIP-1α, predicted a high mortality in sepsis diagnosed according to the ACCP/SCCM criteria.

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Authors and Affiliations

  1. Department of Emergency and Critical Care Medicine, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan

    S. Fujishima, J. Sasaki, Y. Shinozawa, K. Takuma, H. Kimura, M. Suzuki, S. Hori & N. Aikawa

  2. Department of Medicine, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan

    M. Kanazawa

Authors
  1. S. Fujishima
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  2. J. Sasaki
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  3. Y. Shinozawa
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  4. K. Takuma
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  5. H. Kimura
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  6. M. Suzuki
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  7. S. Hori
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  8. N. Aikawa
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  9. M. Kanazawa
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Additional information

This study was supported in part by a Grant-in-Aid from the Japanese Ministry of Education, Science and Culture, the Waxman Institute Research Fund and the Keio Fukuzawa Fund

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Fujishima, S., Sasaki, J., Shinozawa, Y. et al. Serum MIP-1α and IL-8 in septic patients. Intensive Care Med 22, 1169–1175 (1996). https://doi.org/10.1007/BF01709331

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  • DOI: https://doi.org/10.1007/BF01709331

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