Primer sequences and amplicon length of the high-resolution melting experiment
| Primer name | Primer sequence | Genetic region | Fragment length |
|---|---|---|---|
| KRASF2 | TTATAAGGCCTGCTGAAAATGACTGAA | KRAS exon 2 (NC_018923.2) | 92bp |
| KRASR2 | TGAATTAGCTGTATCGTCAAGGCACT | ||
| KRAS61F | CCAGACTGTGTTTCTCCCTT | KRAS exon 3 (NC_018923.2) | 155bp |
| KRAS61R | CACAAAGAAAGCCCTCCCCA | ||
| KRASex4f | TGATTTTGCAGAAAACAGAT | KRAS exon 4 (NC_018923.2) | 120bp |
| KRASex4r | GACACAAAACAGGCTCAGGA | ||
| NRASex.2Fw | GGTGTGAAATGACTGAGTAC | NRAS exon 2 (NC_018912.2) | 128bp |
| NRASex.2Rev | GGGCCTCACCTCTATGGTG | ||
| NRASex.3Fw | AAACAAGTGGTTATAGATGGT | NRAS exon 3 (NC_018912.2) | 97bp |
| NRASex.3Rev | CACAGAGGAAGCCTTCGCCT | ||
| NRASex4f | CTTGCACAAATGCTGAAAGC | NRAS exon 4 (NC_018912.2) | 124bp |
| NRASex4r | TTTGCCAACAAGGACAGTTG | ||
| BRAFex15 F | CCTCAATTCTTACCATCC | BRAF exon 15 NC_018918.2 | 119bp |
| BRAFex15R | ATGAAGACCTCACAGTAA |