Table 1

CanDECIDE study primary objectives and statistical analysis plans

ObjectivesOutcomeIndependent variablesHypothesesStatistical analysis

  1. Measurement of cytokine levels

  2. Gene expression of TLR and cytokine genes

  3. DNA methylation patterns of TLR and cytokine genes

In obese survivors of childhood brain tumours and comparing them with non-obese tumour survivors and lean and obese patients with no history of tumours		 

  1. Cytokine levels

  2. TLR and cytokine gene expression (RNA)

  3. DNA methylation patterns of TLR and cytokine genes

Determine the presence of inflammation and the mechanisms involved in its development in obese survivors of childhood cancer vs controls		 

  1. Age

  2. Sex

  3. Ethnicity

  4. Puberty

  5. Tumour type

  6. Tumour location

  7. Tumour treatment

  8. Hormonal deficiencies

  9. BMI

  10. Lipid levels

  11. HOMA-IR

Obese survivors of childhood brain tumours have higher inflammatory status and altered gene methylation patterns compared with lean survivors and non-cancer controls that predispose them to endometabolic risksRegression

  • BMI, body mass index; CanDECIDE,Canadian Study of Determinants of Endometabolic Health in ChIlDrEn; TLR, Toll-like receptor.