Clinicopathological features, EGFR immunoexpression and CISH/FISH status of the cases
| Case | Age (years)/Gender* | Site | Tumour differentiation | T status | Nodal metastasis | EGFR expression | CISH amplification | FISH amplification | Smoking habit | Alcohol habit | Follow-up† |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 47/M | Tongue | Moderately | T1-T2 | NA | − | − | − | NA | NA | NED |
| 2 | 80/M | Floor of mouth | Well | NA | NA | + | − | − | NA | NA | NED |
| 3 | 55/M | Tongue | Poor | T1-T2 | NA | + | − | − | NA | Yes | NED |
| 4 | 63/M | Tongue | Poor | T1-T2 | NA | + | − | − | NA | NA | NED |
| 5 | 38/M | Floor of mouth | Moderately | NA | NA | − | − | − | NA | NA | NED |
| 6 | 50/M | Floor of mouth | Moderately | T1-T2 | NA | + | − | − | NA | NA | NED |
| 7 | 73/M | Floor of mouth | Poor | T3-T4 | + | − | − | − | < | No | DOD |
| 8 | 59/M | Floor of mouth | Moderately | T3-T4 | − | − | + | + | < | Yes | DOD |
| 9 | 40/F | Floor of mouth | Poor | T1-T2 | − | − | − | − | < | Yes | NED |
| 10 | 57/F | Tongue | Moderately | T1-T2 | NA | − | − | − | < | No | NED |
| 11 | 56/F | Floor of mouth | Poor | T3-T4 | + | − | − | − | < | Yes | NED |
| 12 | 44/M | Floor of mouth | Well | T3-T4 | − | − | + | + | < | Yes | NED |
| 13 | 45/M | Floor of mouth | Moderately | T1-T2 | NA | + | − | − | NA | NA | NED |
| 14 | 38/M | Tongue | Moderately | T1-T2 | NA | + | − | − | NA | Yes | NED |
| 15 | 62/M | Floor of mouth | Well | T1-T2 | − | + | − | − | < | Yes | NED |
| 16 | 54/M | Tongue | Well | T3-T4 | + | + | + | + | < | Yes | NED |
| 17 | 78/M | Tongue | Poor | T1-T2 | NA | + | − | − | NA | No | NED |
| 18 | 79/F | Tongue | Well | T1-T2 | − | + | − | − | No | No | NED |
| 19 | 59/M | Tongue | Well | T3-T4 | − | + | − | − | < | Yes | NED |
| 20 | 49/M | Tongue | Moderately | T1-T2 | − | − | − | − | < | Yes | NED |
| 21 | 16/F | Tongue | Moderately | T1-T2 | + | − | − | − | No | No | NED |
| 22 | 49/M | Floor of mouth | Poor | T3-T4 | + | − | − | − | > | Yes | NED |
| 23 | 80/M | Floor of mouth | Poor | T1-T2 | − | − | − | − | > | Yes | NED |
| 24 | 62/M | Floor of mouth | Poor | T1-T2 | − | − | − | − | < | Yes | NED |
| 25 | 61/M | Floor of mouth | Poor | T1-T2 | − | + | − | − | > | No | NED |
| 26 | 72/F | Tongue | Well | T1-T2 | + | + | − | + | No | No | NED |
| 27 | 78/F | Tongue | Well | T1-T2 | − | + | − | + | No | No | NED |
| 28 | 72/F | Tongue | Poor | T1-T2 | + | − | − | − | No | No | DOD |
| 29 | 48/M | Tongue | Poor | T3-T4 | + | − | − | − | No | No | NED |
| 30 | 52/F | Tongue | Poor | T1-T2 | + | − | − | − | < | Yes | NED |
| 31 | 46/M | Tongue | Poor | T3-T4 | + | − | − | − | < | Yes | NED |
| 32 | 61/M | Tongue | Poor | T3-T4 | + | − | − | − | > | Yes | NED |
| 33 | 63/M | Tongue | Moderately | T1-T2 | + | + | − | − | < | Yes | NED |
| 34 | 69/M | Floor of mouth | Poor | T3-T4 | + | + | − | − | > | No | NED |
| 35 | 45/M | Floor of mouth | Well | NA | NA | + | − | − | < | Yes | NED |
| 36 | 58/M | Floor of mouth | Moderately | T3-T4 | + | + | − | − | < | Yes | NED |
| 37 | 52/M | Tongue | Well | T1-T2 | + | + | − | − | No | No | NED |
| 38 | 64/M | Floor of mouth | Moderately | T1-T2 | − | + | − | − | NA | NA | DOD |
| 39 | 49/M | Tongue | Well | T1-T2 | + | + | − | + | NA | NA | NED |
| 40 | 51 / M | Gum | Moderately | NA | NA | + | − | + | NA | Yes | NED |
| 41 | 40/F | Palate | Well | T1-T2 | NA | − | − | − | NA | Yes | NED |
| 42 | 54/M | Gum | Well | T1-T2 | NA | − | − | − | NA | Yes | NED |
| 43 | 44/M | Gum | Poor | NA | NA | + | − | + | NA | Yes | NED |
| 44 | 51/M | Gum | Poor | T1-T2 | − | − | − | − | NA | Yes | NED |
| 45 | 51/M | Gum | Well | T1-T2 | NA | − | − | − | NA | NA | NED |
| 46 | 68/M | Palate | Poor | T1-T2 | − | + | − | − | NA | No | NED |
| 47 | 62/M | Gum | Well | T1-T2 | NA | + | − | − | NA | Yes | NED |
| 48 | 62/F | Gum | Well | T1-T2 | − | + | − | − | < | No | DOD |
| 49 | 44/M | Palate | Poor | T3-T4 | + | + | − | − | > | Yes | NED |
| 50 | 77/M | Gum | Poor | NA | NA | + | − | − | NA | NA | NED |
| 51 | 53/F | Buccal mucosa | Well | T1-T2 | + | + | − | − | No | No | NED |
| 52 | 48/M | Gum | Moderately | T3-T4 | + | − | − | − | < | Yes | DOD |
CISH, chromogenic in situ hybridization; DOD, dead of disease; EGFR, epidermal growth factor receptor; F, female; FISH, fluorescence in situ hybridization; M, male; NA, not available; NED, no evidence of disease.
*(−) negative, (+) positive, < under 20 cigarettes/day, > above 20 cigarettes/day.
†Mean 6 months.