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Relationship between clinical diagnosis of recurrent vulvovaginal candidiasis and detection of Candida species by culture and polymerase chain reaction

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Abstract

Background

Recurring vulvovaginal candidiasis (RVVC) is a common vaginal discharge affecting 75% of all women at least once in their life. In 5% of these women, infection is recurring. Aim of the study was to determine the sensitivity of detecting Candida species by culture and polymerase chain reaction (PCR) in women with a clinical diagnosis of RVVC.

Methods

A total number of 104 patients referred with a clinical diagnosis of RVVC and therefore at least four episodes in the previous year were evaluated. In order to detect Candida, vaginal swabs were cultured on Sabouraud and chromagar. Furthermore, the supernatant from the vaginal lavage was examined for the presence of Candida by PCR.

Results

When the culture was analyzed, only 31 (29.8%) of the 104 patients diagnosed with RVVC were positive for Candida species in their vagina. Candida albicans was identified in 25 women and six were positive for Candida glabrata. When analyzed by PCR, 44 (42.3%) patients were positive for Candida species. In 13 women (12.5%) only the PCR was positive, while in 31 patients both culture and PCR were positive.

Conclusion

The diagnostic method of PCR is more sensitive than culture in detecting Candida species in the vagina. The results also suggest further investigation to verify the complaints of the negative tested patients.

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References

  1. Barousse MM, Steele C, Dunlap K, Espinosa T, Boikov D, Sobel JD, Fidel PL Jr (2001) Growth inhibition of Candida albicans by human vaginal epithelia cells. J Infect Dis 184(11):1489–1493. doi: 10.1086/324532

    Article  PubMed  CAS  Google Scholar 

  2. Dan M, Kaneti N, Levin D, Poch F, Samara Z (2003) Vaginitis in a gynecologic practice in Israel: causes and risk factors. Isr Med Assoc J 5(9):629–632

    PubMed  Google Scholar 

  3. Giraldo P, von Nowaskonski A, Gomes FA, Linhares I, Neves NA, Witkin SS (2000) Vaginal colonization by Candida in asymptomatic women with and without a history of recurrent vulvovaginal candidosis. Obstet Gynecol 95(3):413–416. doi:10.1016/S0029-7844(99)00577-3

    Article  PubMed  CAS  Google Scholar 

  4. Linhares LM, Witkin SS, Miranda SD, Fonseca AM, Pinotti JA, Ledger WJ (2001) Differentiation between women with vulvovaginal symptoms who are positive or negative for Candida species by culture. Infect Dis Obstet Gynecol 9(4):221–225. doi:10.1155/S1064744901000369

    Article  PubMed  CAS  Google Scholar 

  5. Maccato ML, Kaufman RH (1991) Fungal vulvovaginitis. Curr Opin Obstet Gynecol 3(6):849–852. doi:10.1097/00001703-199112000-00018

    PubMed  CAS  Google Scholar 

  6. Mardh PA, Rodrigues AG, Genc M, Novikova N, Martinez-de-Oliviera J, Guaschino S (2002) Facts and myths on recurrent vulvovaginal candidosis—a review on epidemiology, clinical manifestations, diagnosis, pathogenesis and therapy. Int J STD AIDS 13(8):522–539. doi: 10.1258/095646202760159639

    Article  PubMed  Google Scholar 

  7. Mendling W, Krauss C, Fladung B (2004) A clinical multicenter study comparing efficacy and tolerability of topical combination therapy with clotrimazole (Canesten, two formats) with oral single dose fluconazole (Diflucan) in vulvovaginal mycoses. Mycoses. 47(3–4):136–142. doi:10.1111/j.1439-0507.2004.00970.x

    Article  PubMed  CAS  Google Scholar 

  8. Metzner G, Weissenbacher ER (1999) Candidainfektionen des weiblichen Genitaltraktes. Medifact publishing, München, 1, Auflage

  9. Rosedale N, Browne K (1979) Hyposensitation in the management of recurring vaginal candidosis. Ann Allergy 43(4):250–253

    PubMed  CAS  Google Scholar 

  10. Saporiti AM, Gomez D, Levalle S, Galeano M, Davel G, Vivot W, Rodero L (2001) Vaginal candidosis: etiology and sensitivity profile to antifungal agents in clinical use. Rev Argent Microbiol 33(4):217–222

    PubMed  CAS  Google Scholar 

  11. Sobel JD (2002) Treatment of vaginal Candida infections. Expert Opin Pharmacother 3(8):1059–1065. doi: 10.1517/14656566.3.8.1059

    Article  PubMed  CAS  Google Scholar 

  12. Wilton L, Kollarova M, Heeley E, Shakir S (2003) Relative risk of vaginal candidosis after use of antibiotics compared with antidepressants in women: postmarketing surveillance data in England. Drug Saf 26(8):589–97. doi:10.2165/00002018-200326080-00005

    Article  PubMed  CAS  Google Scholar 

  13. Xiang H, Xiong L, Liu X, Tu Z (2007) Rapid simultaneous detection and identification of six species Candida using polymerase chain reaction and reverse line hybridization assay. J Micribiol Methods 69(2):282–287. doi:10.1016/j.mimet.2007.01.011

    Article  CAS  Google Scholar 

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Acknowledgment

We express our gratitude to U. Jeschke, Ph.D and G. Anton Ph.D for their help with the manuscript.

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Authors and Affiliations

  1. First Department of Obstetrics and Gynecology, Klinikum Innenstadt, Ludwig-Maximilians-University, Maistrasse 11, 80337, Munich, Germany

    T. Weissenbacher, A. Gingelmaier, C. Scholz, K. Friese & I. Mylonas

  2. Department of Obstetrics and Gynecology, Klinikum Großhadern, Ludwig-Maximilians-University of Munich, Munich, Germany

    V. Tolbert, E. R. Weissenbacher & K. Friese

  3. Department of Obstetrics and Gynecology, Cornell University Weill Medical College, New York, USA

    S. S. Witkin & W. J. Ledger

Authors
  1. T. Weissenbacher
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  2. S. S. Witkin
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  3. W. J. Ledger
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  4. V. Tolbert
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  5. A. Gingelmaier
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  6. C. Scholz
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  7. E. R. Weissenbacher
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  8. K. Friese
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  9. I. Mylonas
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Corresponding author

Correspondence to I. Mylonas.

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Weissenbacher, T., Witkin, S.S., Ledger, W.J. et al. Relationship between clinical diagnosis of recurrent vulvovaginal candidiasis and detection of Candida species by culture and polymerase chain reaction. Arch Gynecol Obstet 279, 125–129 (2009). https://doi.org/10.1007/s00404-008-0681-9

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  • DOI: https://doi.org/10.1007/s00404-008-0681-9

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