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113: SPECIFIC TARGETING OF HER2-POSITIVE BREAST CANCER CELL LINE (SK-BR3) USING IDARUBICIN-LOADED ANTI-HER2 IMMUNOLIPOSOME
  1. Aminollah Pourshohod1,
  2. Majid Zeinali2,
  3. Moslem Afrakhte1,
  4. Mostafa Jamalan3
  1. 1Department of Biochemistry, Cellular and Molecular Research Center, Ahvaz Jundishapur University of medical science, Medical School, Ahvaz, Iran
  2. 2Biotechnology Research Center, Research Institute of Petroleum Industry (RIPI), Tehran, Iran
  3. 3Abadan School of Medical Sciences, Abadan, Iran

Abstract

Cytotoxic chemotherapy drugs which are used for treating malignant cells cannot distinguish between normal and cancerous cells. This would lead to adverse side effects. Moreover, drug resistance is another major problem of the classical chemotherapy during the treatment period. Recently, various therapeutic strategies have been developed for cell-specific targeting of anti-neoplastic drugs. Among the various kinds of drug delivery systems, (immuno) liposome has attracted high attention for its unique features such as high loading capacity, lower toxicity, delivery of soluble/non-soluble drugs, targeting and versatile structural specifications that permit easy surface decoration. Idarubicin is a semisynthetic anticancer drug that is widely used for treatment of leukemia as well as other kinds of cancers.

In this study, liposomes were covered with biocompatible polyethylene glycol (PEG) and dress up with a monoclonal antibody (mAb) Trastuzumab (Herceptin®). Trastuzumab is a humanized mAb approved by FDA for ablation of HER-2 over express cells in metastatic breast cancer. The constructed immunoliposome was loaded by Idarubicin. Shape, polarity, and size of synthesized immunoliposomes were characterized by the Atomic Force Microscopy (AFM) and Dynamic Light scattering (DLS) methods. SK-BR3 and MCF7 cell lines were used as HER2- positive and HER2-negative cells, respectively. Cell lines were treated with different concentrations of Idarubicin either in the free form or entrapped in liposomes/ immunoliposomes. After treatment, growth of malignant cells was evaluated by MTT assay.

Mean diameter and zeta potential of the prepared Idarubicin-loaded anti-HER2 immunoliposomes were 160 nm and –20 mV, respectively. Cell lines responded to Idarubicin in a concentration dependent manner. According to the obtained results, Idarubicin showed potent toxic effect on both kinds of cell lines, however; SK-BR3 cells were significantly more sensitive. In comparison, Idarubicin in the form of immunoliposome was more potent in the killing of SK-BR3 malignant cells.

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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