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Abstract
Introduction A substantial majority of chronic obstructive pulmonary disease (COPD)-related morbidity, mortality and healthcare costs are due to acute exacerbations, but existing medications have only a modest effect on reducing their frequency, even when used in combination. Observational studies suggest β-blockers may reduce the risk of COPD exacerbations; thus, we will conduct a randomised, placebo-controlled trial to definitively assess the impact of metoprolol succinate on the rate of COPD exacerbations.
Methods and analyses This is a multicentre, placebo-controlled, double-blind, prospective randomised trial that will enrol 1028 patients with at least moderately severe COPD over a 3-year period. Participants with at least moderate COPD will be randomised in a 1:1 fashion to receive metoprolol or placebo; the cohort will be enriched for patients at high risk for exacerbations. Patients will be screened and then randomised over a 2-week period and will then undergo a dose titration period for the following 6 weeks. Thereafter, patients will be followed for 42 additional weeks on their target dose of metoprolol or placebo followed by a 4-week washout period. The primary end point is time to first occurrence of an acute exacerbation during the treatment period. Secondary end points include rates and severity of COPD exacerbations; rate of major cardiovascular events; all-cause mortality; lung function (forced expiratory volume in 1 s (FEV1)); dyspnoea; quality of life; exercise capacity; markers of cardiac stretch (pro-NT brain natriuretic peptide) and systemic inflammation (high-sensitivity C reactive protein and fibrinogen). Analyses will be performed on an intent-to-treat basis.
Ethics and dissemination The study protocol has been approved by the Department of Defense Human Protection Research Office and will be approved by the institutional review board of all participating centres. Study findings will be disseminated through presentations at national and international conferences and publications in peer-reviewed journals.
Trial registration number NCT02587351; Pre-results.
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Strengths and limitations of this study
Although numerous observational studies show a positive association between the use of β-blockers and the reduction in chronic obstructive pulmonary disease (COPD) exacerbations, this study will be the first prospective randomised, double-blind, placebo-controlled trial to examine the issue.
In addition to collecting data about the occurrence of acute exacerbations, we will also collect major adverse cardiac events allowing examination of the effects of β-blockers on pulmonary and cardiovascular outcomes.
We will specifically exclude patients with recent cardiovascular events in whom it is likely that β-blockers would be most effective. However, we will perform subgroup analyses based on predicted cardiovascular risk as defined by the Personal HEART Score in an effort to identify those patients most likely to benefit.
The optimal dose of metoprolol for the prevention of exacerbations in COPD is unknown, and it is possible that the median dose we achieve will be too low to be beneficial.
The study is not powered to detect an effect on overall mortality which we believe would be the best end point to objectively assess the role of the drug in patients with COPD.
Introduction
A substantial majority of chronic obstructive pulmonary disease (COPD)-related healthcare costs are due to acute exacerbations.1 ,2 The proportional costs associated with exacerbations continue to rise and existing medications have only a modest effect on reducing their frequency, even when used in combination.1 ,3 There is therefore an urgent need for more effective therapies targeting exacerbations. Development of such treatment has been hampered by the heterogeneity of these events, which though often triggered by airway inflammation due to bacterial or viral infections or exposure to pollutants can also be caused or made worse by cardiovascular disease, a factor likely not impacted by currently available bronchodilator and anti-inflammatory medications.4 ,5
There is a growing awareness that COPD is a multisystem disease and that it is associated with accelerated atherosclerosis and cardiovascular disease.4 A significant number of cardiac comorbidities which could potentially result in acute decompensation of respiratory status such as coronary artery disease, diastolic dysfunction and arrhythmias are seen in greater frequency in patients with COPD compared with age-matched and sex-matched controls.4 ,5 There is also growing evidence for cardiac injury in the periexacerbation period, and this relationship is likely bidirectional with some of the exacerbations caused by cardiac events.6
Multiple observational studies have suggested that existing cardiac medications can improve survival in patients with COPD and also reduce the rate of exacerbations, and these potential benefits are perhaps most pronounced for β-blockers.6–12 Despite concerns that these drugs may cause bronchoconstriction, the weight of the data suggests that this fear may be misplaced, at least for cardioselective β-blockers. Studies examining the effects of cardioselective β-blockers have found no consistently deleterious effect on lung function. Although forced expiratory volume in 1 s (FEV1) declines significantly with non-selective β-blockers,13 ,14 cardioselective β-blockers do not reduce FEV1 either acutely or with long-term use.13 ,15 Gottlieb et al demonstrated that the survival benefits associated with β-blocker use post myocardial infarction are as significant for those with COPD as compared with those without the disease.7 Rutten et al8 have shown that patients on β-blockers have a significant reduction in exacerbation frequency, and we and others have found comparable results in multiple similar observational cohorts.8–11 These observations are biologically plausible as in addition to their established cardioprotective effects which could impact the risk of acute exacerbations or their severity, β-blockers may also have beneficial respiratory effects.12 These results are tempered by the results of Ekström et al16 who showed increased mortality in patients with severe COPD and on home oxygen who were taking β-blockers; in contrast, in the COPDGene study, we found a greater beneficial effect on exacerbation frequency in this subgroup.9
Though the observational data suggesting that β-blockers may reduce exacerbations are compelling, these studies are all subject to a number of inherent biases that preclude conclusions about cause and effect. In addition, though the published data do not show a meaningful effect of cardioselective β-blockers on lung function, these drugs are significantly underprescribed in patients with COPD, even when they have absolute indications for their use, suggesting practitioners still have concerns about their safety.
To address these issues, we have designed a randomised, placebo-controlled trial, the βLOCK COPD study, to examine the effect of extended-release metoprolol on the rate of exacerbations in patients with COPD at high risk for those events (NCT02587351). We will test the hypothesis that treatment with a cardioselective β-blocker will reduce the time to first exacerbation and exacerbation frequency and that the drug will be well tolerated and not adversely affect lung function, exercise tolerance and quality of life. In this article, we describe the study design, discuss the rationale for the specific approaches employed and outline the prespecified subgroup analyses.
Methods
βLOCK COPD study design overview
This is a multicentre, placebo-controlled, double-blind, prospective randomised trial that will enrol 1028 patients with at least moderately severe COPD over a 3-year period. Patients will be screened and then randomised over a 2-week period and will then undergo a dose titration of metoprolol for the following 6 weeks. Thereafter, patients will be followed for 42 additional weeks on their target dose of metoprolol or placebo followed by a 4-week washout period. The schedule of study encounters is shown in table 1.
Schedule of study interventions
Hypothesis
The primary hypothesis is that metoprolol succinate will reduce the risk of COPD exacerbations as compared with placebo in patients with moderate-to-severe COPD who are prone to exacerbations and who do not have absolute indications for β-blocker therapy. The secondary hypothesis is that metoprolol succinate will not adversely impact lung function, exercise tolerance, dyspnoea or quality of life as compared with placebo.
Inclusion and exclusion criteria
We will enrol patients aged 40–85 years with a clinical diagnosis of at least moderate COPD as defined by the Global Initiative for Chronic Obstructive Lung Disease criteria of postbronchodilator FEV1/forced vital capacity (FVC)<0.70 and postbronchodilator FEV1<80% predicted, with or without chronic symptoms such as cough and sputum production. Participants should have a cigarette smoking history of at least 10 pack-years. The study will be enriched for patients at high risk for exacerbations as suggested by at least one of the following: a history of having received a course of systemic corticosteroids and/or antibiotics for respiratory events in the past year, having visited an emergency department for a COPD exacerbation within the past year, hospitalisation for COPD exacerbation within the past year or using or have been prescribed supplemental home oxygen for at least 12 hours a day.17 ,18 Participants should have a resting heart rate of at least 70 and not >120 bpm, and resting systolic blood pressure of >100 mm Hg to be eligible. Major exclusion criteria are listed in box 1 and include the presence of an absolute indication for a β-blocker though patients with stable coronary disease or mild systolic dysfunction with left ventricular ejection fraction >40% can be included.
Exclusion criteria
A diagnosis of asthma established by each study investigator on the basis of the recent American Thoracic Society/European Respiratory Society and National Institute for Health and Care Excellence guidelines.19 ,20 If, after applying the above criteria, investigators are still unsure about the distinction in a specific patient, bronchodilator testing with inhaled albuterol will be performed and patients with changes in forced expiratory volume in 1 s (FEV1) >400 mL will be excluded.
The presence of a diagnosis other than chronic obstructive pulmonary disease (COPD) that results in the patient being either medically unstable or having a predicted life expectancy <2 years.
Women who are at risk of becoming pregnant during the study (premenopausal) and who refuse to use acceptable birth control (hormone-based oral or barrier contraceptive) for the duration of the study.
Current tachyarrhythmias or bradycardia requiring treatment.
Presence of a pacemaker and/or internal cardioverter/defibrillator.
Patients with a history of second-degree or third-degree (complete) heart block, or sick sinus syndrome.
Baseline ECG revealing left bundle branch block, bifascicular block, ventricular tachyarrhythmia, atrial fibrillation, atrial flutter, supraventricular tachycardia (other than sinus tachycardia and multifocal atrial tachycardia) or heart block (second degree or complete).
Resting heart rate <70 bpm, or sustained resting tachycardia defined as heart rate >120 bpm.
Resting systolic blood pressure of <100 mm Hg.
Participants with absolute (class 1) indications for β-blocker treatment as defined by the combined American College of Cardiology Foundation/American Heart Association Task Force on Practice Guidelines, and the American College of Physicians, American Association for Thoracic Surgery, Preventive Cardiovascular Nurses Association, Society for Cardiovascular Angiography and Interventions, and Society of Thoracic Surgeons Guidelines which include myocardial infarction, acute coronary syndrome, percutaneous coronary intervention or coronary artery bypass surgery within the prior 3 years and patients with known congestive heart failure defined as left ventricular ejection fraction <40%.21 ,22
Current therapy with ocular β-blocker medications.
Critical ischaemia related to peripheral arterial disease.
Other diseases that are known to be triggered by β-blockers or β-blocker withdrawal including myasthenia gravis, periodic hypokalemic paralysis, pheochromocytoma and thyrotoxicosis.
Patients on other cardiac medications known to cause atrioventricular (AV) node conduction delays such as amiodarone, digoxin and calcium channel blockers including verapamil and diltiazem as well as patients taking clonidine.
Hospitalisation for uncontrolled diabetes mellitus or hypoglycaemia within the last 12 months.
Patients with cirrhosis.
A clinical diagnosis of bronchiectasis defined as production of greater than one-half cup of purulent sputum per day.
Patients otherwise meeting the inclusion criteria will not be enrolled until they are a minimum of 4 weeks from their most recent acute exacerbation (ie, they will not have received a course of systemic corticosteroids, an increased dose of chronically administered systemic corticosteroids and/or antibiotics for an acute exacerbation for a minimum of 4 weeks).
Randomisation and intervention
After obtaining written informed consent, randomisation will be performed according to a computer-generated blinded algorithm carried out by linking to the Data Coordinating Center (DCC) through a website (beta.umn.edu/betablocker.umn.edu) using a required user ID and password. The clinical trial will use metoprolol succinate extended-release tablets (50 mg) and matching placebo. Drug and matching placebo will be labelled using blinded coding and distributed to the study sites as needed to support enrolment and retention. The planned starting dose for metoprolol succinate extended release or placebo equivalent is one 50 mg tablet taken orally daily, and patients will undergo a dose titration procedure as outlined in table 2, which will result in a final dose of 25 mg (1/2 of one tablet daily), 50 mg or 100 mg (two tablets daily). Matching placebo will be administered similarly. Following completion of the 42-week dosing period, patients will be weaned off study drug over the following 4 weeks in order to avoid possible rebound myocardial ischaemia.
Dose adjustment protocol
Clinical efficacy: primary and secondary outcomes
The primary end point is time to first occurrence of an acute exacerbation during the 48-week treatment period. Acute exacerbations will be defined as a ‘complex of respiratory symptoms (increase or new onset) of more than one of the following: cough, sputum, wheezing, dyspnea, or chest tightness requiring treatment with antibiotics and/or systemic steroids for at least three days’.23 Severe exacerbations will be defined as those exacerbations that require hospitalisation. A relapse of a previous exacerbation will be defined as the complex of respiratory symptoms of more than one of the following: cough, sputum, wheezing, dyspnoea or chest tightness with a duration of at least 3 days, which recurs and requires retreatment with antibiotics and/or systemic steroids without a return to baseline and within 2 weeks of the start date of a prior treated event.24 Secondary end points include rates and severity of COPD exacerbations; rate of major cardiovascular events ((major adverse cardiac event (MACE) defined by cardiovascular death, hospitalisation for myocardial infarction, heart failure or stroke), percutaneous coronary intervention or coronary artery bypass grafting); the combined rate of exacerbations and MACE; all-cause mortality; lung function (FEV1); dyspnoea as measured by the modified Medical Research Council and San Diego Shortness of Breath Questionnaire Score; quality of life as measured by the Short Form-36 (SF-36), St George Respiratory Questionnaire and the COPD Assessment Test scores; exercise capacity measured by the 6 min walk distance; markers of cardiac stretch (pro-NT Brain Natriuretic Peptide) and systemic inflammation (high-sensitivity C reactive protein and fibrinogen). These parameters will be assessed at screening/randomisation and at conclusion of the study to determine if β blockade impacts volume status and cardiac performance as well as levels of systemic inflammation that portend overall cardiac risk.
Adverse effects and safety monitoring: prior studies of β-blockers and effects on lung function and exercise
Several studies have examined the safety of β-blockers in patients with COPD, though there have been no dose-ranging studies to specifically determine the optimal dose for the prevention of exacerbations. Multiple studies have demonstrated that the effect of cardioselective β-blockers on lung function is minimal, whether administered as a single dose or with continued treatment. A Cochrane analysis revealed that cardioselective β-blockers produced no significant change in FEV1 or respiratory symptoms compared with placebo, given as a single dose (−2.05% (95% CI −6.05% to 1.96%)) or for longer duration (−2.55% (CI −5.94% to 0.84%)), and did not significantly affect the FEV1 treatment response to β2 agonists.15 ,25 Subgroup analyses revealed no significant change in the results for those participants with severe airflow limitation or for those with a reversible obstructive component. Typical doses of metoprolol in trials of patients with coronary artery disease, congestive heart failure and hypertension range from 12.5 to 200 mg, and doses in this range are well tolerated by patients with COPD including those with moderate-to-severe disease.25 The dose titration procedure is modelled after the approach used in a pivotal trial of metoprolol succinate in patients with heart failure.26 In that study, in which daily doses of up to 200 mg (mean dose 159 mg once daily) were used, 10.3% of 1990 patients assigned to metoprolol succinate extended-release tablets discontinued for adverse reactions versus 12.2% of placebo patients. Adverse events that occurred at an incidence of ≥1% in the metoprolol succinate extended-release tablets group and greater than placebo by >0.5% (and regardless of causality) included dizziness/vertigo (1.8% vs 1.0%), bradycardia (1.5% vs 0.4%) and accident and/or injury (1.4% vs 0.8%). The planned median daily dose of metoprolol in the proposed trial will fall between 50 and 100 mg, and these as well as a number of other possible drug-related side effects will be specifically sought and recorded. We will monitor FEV1 during the dose titration period, and patients whose FEV1 falls by >200 mL or 15% from baseline will be taken off study drug.
Discontinuation of study drug
There are four instances in which the study drug might be discontinued: (1) development of symptoms that might represent medication-related side effects that are severe enough or persist even with dose reduction; (2) development of an absolute indication for β-blocker such as myocardial infarction, acute coronary syndrome, percutaneous coronary intervention, coronary artery bypass surgery or new congestive heart failure with ejection fraction <40%. In these instances, study medication will be stopped and the patient referred for appropriate medical treatment; (3) intercurrent illness including medical and/or surgical problems that are unrelated to COPD or to a possible metoprolol-related side effect but warrant treatment. In these instances, the patient's treating physician (or study physician) will decide whether the specific problem encountered warrants discontinuation of the study medication. Each patient will carry a wallet card for the duration of the study that provides information regarding the study and how unmasking of treatment can be accomplished should the indication merit and (4) new prescription of a contraindicated medication (box 1).
Statistical analyses
Sample size and power considerations for this clinical trial are based on the primary outcome of time to first exacerbation. The risk of exacerbation and estimated time to first exacerbation in the placebo group is based on the observations in the control groups of the prior COPD Clinical Research Network trials of azithromycin and simvastatin of similar design.17 ,18 The percentage of patients suffering an exacerbation at 1 year in the placebo arm of the azithromycin trial was 69% compared with 57% in those receiving azithromycin. In the simvastatin trial, the probability of patients in the placebo arm suffering an exacerbation was 65%, while the probability in those taking simvastatin was not statistically different (68%). With similar inclusion and exclusion criteria as these prior trials, we anticipate a comparable exacerbation rate. Prior observational studies suggest that β-blockers may reduce the risk of exacerbation by as much as 30%, though it is probable that this overestimates the potential benefit due to residual confounding. We believe a 15% relative reduction (65% vs 55%) in the 48-week-period probability of exacerbation is clinically significant and plausible and have thus selected that as our hypothesised effect size. To find this effect, with a two-sided α of 0.05 and power of 90%, and equal probability of assignment to either arm, we will need a sample size of 912 participants, assuming 12% dropout yields a final sample size of 1028 patients.
All randomised patients will be followed until the end of the study, and the final analysis will be performed on an ‘intention-to-treat’ basis. The analyses of the time to first COPD exacerbation (and all-cause mortality) will be performed using survival analysis. Kaplan–Meier survival curves will be used to describe the probability of remaining outcome-free in the two treatment arms as a function of time from randomisation into the study. The curves will be compared using the log-rank test statistic. Secondary outcome measures will be assessed at baseline, week 16/day 112 and week 48/day 336. COPD exacerbation rates will be calculated as events/person-year and compared using a rate ratio. Exacerbation rates for each group, and the resultant rate ratio, will be analysed using negative binomial regression modelling. The model will employ time-weighted intention-to-treat analyses with adjustments of the CIs for between-subject variation and overdispersion.27 ,28 Continuous outcome measures, including absolute and per cent changes in FEV1, 6 min walk distance, dyspnoea and quality-of-life scores, will be analysed using multivariate repeated-measures analysis of variance using the SAS Proc Mixed program.
We propose to carry out interim formal testing at the following time points: 12 months and 24 months, and 36 months after initiating the study. We will use the Lan-DeMets approach that requires only specification of the rate at which type I error (which here will be chosen to be α=0.05) will be ‘spent’. Two-sided tests of significance will be assumed.
Planned subgroup analyses
Using the approach outlined for primary and secondary analyses, we will perform two subgroup analyses for (1) cardiovascular risk based on the Personal HEART Score29 and (2) age greater versus <65. These analyses will primarily be hypothesis generating in nature.
Discussion
There is an urgent need for new therapies to reduce exacerbations as existing drugs offer only modest effects even when used in combination and only target bronchoconstriction and airway inflammation when other pathways likely contribute. Stable COPD is strongly associated with cardiovascular disease independent of shared risk factors such as cigarette smoking and age,4 and there is growing evidence that acute exacerbations of COPD are associated with cardiac injury.6 It is biologically plausible that the relationship between respiratory decompensation and cardiac affectation is not unidirectional and that a subset of the exacerbations might be cardiac in aetiology. Patel et al showed that arterial stiffness, a surrogate for cardiovascular risk, increases in the periexacerbation period and takes up to 5 weeks to return to baseline.6 They also showed that subclinical increases in troponin I, a marker of cardiac injury, occur in the periexacerbation period even in patients without known cardiovascular disease.
There are a number of mechanisms by which subclinical cardiac dysfunction can result in COPD exacerbations which are clinically very difficult to distinguish from usual, primary respiratory-related events. In addition to a higher frequency of ischaemic heart disease, COPD is associated with diastolic dysfunction in a substantial proportion of patients and decompensated diastolic dysfunction can result in subclinical pulmonary congestion.30–32 Supraventricular and ventricular arrhythmias are common in COPD, and arrhythmias might also cause acute exacerbations.33 The heightened resting sympathetic activity in COPD has been associated with mortality and β-blockers might alleviate some of this risk by reducing resting tachycardia and arrhythmias.34–36 β-Blockers may also improve outcomes by decreasing arrhythmogenesis and myocardial ischaemia associated with excessive use of β agonists during periods leading up to and during exacerbation.37 In addition to their known cardioprotective effects, β-blockers might also have beneficial effects on the lungs. Murine models suggest that long-term administration of β-blockers results in upregulation of pulmonary β adrenoreceptors,12 as well as decreased bronchoconstriction and an improved response to β agonists.38 Chronic administration also has been shown in animal studies to reduce airway inflammation and decrease mucus production.39 Some cardioselective β-blockers can also cause pulmonary vasodilation and thus improve pulmonary haemodynamics.40
The selection of metoprolol, a cardioselective agent, as the β-blocker of choice for the trial merits some discussion as does the proposed dosing. Though less cardioselective agents such as carvedilol may offer greater cardioprotective effects, concerns regarding adverse effects on FEV1 and the risk of respiratory decompensation are greater with these drugs.13 ,14 ,41 ,42 It is also possible that the cardiac benefit of β-blockers in COPD is due to heart rate control and metoprolol has very low intrinsic sympathomimetic activity.36 Cardioselectivity for all β-blockers is dose dependent and at higher doses, even selective drugs can result in clinically significant antagonism of β2 receptors.36 The initial dose of metoprolol and subsequent titration procedures are adapted from the landmark Metoprolol CR/XL Randomized Intervention Trial in Congestive Heart Failure trial, which definitively demonstrated the safety and efficacy of β blockade in patients with symptomatic heart failure, a disease that similar to COPD had been previously considered a contraindication to β-blocker treatment.26 This study suggested that individualised dosing based on patient tolerability was appropriate, but titration to a dose above 100 mg/day may not be necessary to derive clinical benefits as there was no difference in mortality between those who received higher versus lower doses.26 Our initial starting dose of metoprolol is based on these data as well as prior studies in patients with COPD, suggesting tolerance with single and continued dosing at comparable doses of the drug and other cardioselective β-blockers.15 ,25 ,42–44 It is anticipated that many patients will tolerate titration to the maximal dose of 100 mg/day, while some will require a dose reduction to remain on study medication.
The trial design has some important limitations. First, it is likely that β-blockers would be most effective in patients with recent cardiovascular events whom we will specifically exclude; however, we will perform subgroup analyses based on predicted cardiovascular risk as defined by the Personal HEART Score in an effort to identify those patients most likely to benefit.29 Second, as we have discussed, the optimal dose of metoprolol for the prevention of exacerbations in COPD is unknown, and it is possible that the median dose we achieve will be too low to be beneficial. It is also possible the drug will be poorly tolerated and frequently stopped due to side effects in which case a possible beneficial effect on exacerbations will not be found. Last, the study is not powered to detect an effect on overall mortality which we believe would be the best end point to objectively assess the role of the drug in patients with COPD.
In summary, the βLOCK COPD study will be the first randomised controlled study to investigate the effect of β-blockers on COPD exacerbations. By assessing clinical efficacy as well as side effects, the data obtained may guide β-blocker use in COPD.
Ethics and dissemination
The study protocol has been approved by the Department of Defense Human Protection Research Office and will be approved by the institutional review board (IRB) of all participating centres. The trial is registered at ClinicalTrials.Gov (http://www.clinicalstrials.gov identifier NCT02587351). After explaining the risks and benefits of participating in the study, written informed consent will be obtained from each study participant.
Clinical trial monitoring to ensure the trial is conducted in compliance with Good Clinical Practices and the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH E6) will be multifaceted, including real-time oversight by the local principal investigators, regular and real-time monitoring of entered clinical data by staff at the DCC, as well as by an independent Data and Safety Monitoring Committee (DSMC) which will meet at 6-month intervals by teleconference or in person.45 The DSMC will be made up of a lead Research Monitor (a pulmonologist), a cardiologist and a statistician. The Research Monitor will oversee the safety of the research and report observations and findings to the IRB or a designated institutional official. The Research Monitor will review all unanticipated problems involving risks to participants or others associated with the protocol and provide an independent report of the event to the IRB. The Research Monitor may discuss the research protocol with the investigators; shall have authority to stop a research protocol in progress, remove individual human participants from a research protocol and take whatever steps are necessary to protect the safety and well-being of human participants until the IRB can assess the monitor's report; and shall have the responsibility to promptly report their observations and findings to the IRB or other designated official and the Human Research Protection Office. The DCC will conduct monthly teleconferences throughout the study to review study enrolment and retention, procedures, adherence to protocol, timeliness of data entry and adverse events including those that may warrant protocol changes.
Study findings will be disseminated through presentations at national and international conferences and publications in peer-reviewed journals.
References
Footnotes
Contributors MTD and SPB conceived and designed the study. MTD drafted the grant proposal. SPB and MTD drafted the protocol presented. JEC, HV, SML and EW provided methodological and statistical support. MTD, JEC, EW, HV and SML are responsible for study management, staff training and supervision. SCL and GJC are directors of two of the recruitment sites and provided clinical expertise and on-site management of the study. JMW reviewed the manuscript for critical intellectual input. All authors critically reviewed and approved the final version of the manuscript.
Funding The study is funded by a grant from the Department of Defense Congressionally Directed Medical Research Program: Peer Reviewed Medical Research Program—Clinical Trial Award PR140170—GRANT11766881.
Competing interests SPB is supported by NIH KL2 Scholarship, 1KL2TR001419. JMW has grants from the NIH/NHLBI and the Cystic Fibrosis Foundation; his institution has grants for research from GlaxoSmithKline and AstraZeneca. MTD has received grant support from the American Heart Association, the Department of Defense and the NHLBI. He has served as a consultant for Boehringer Ingelheim, Boston Scientific, Ikaria, GlaxoSmithKline and Skyepharma. He has conducted contracted clinical trials for Aeris, AstraZeneca, Boehringer Ingelheim, Boston Scientific, GlaxoSmithKline, Otsuka, Pearl, Pulmonx, PneumRx/BTG and Yungjin. JEC, HV and SML have no conflicts of interest to declare.
Ethics approval IRBs of all participating institutions.
Provenance and peer review Not commissioned; peer reviewed for ethical and funding approval prior to submission.
Data sharing statement Pending approval from the Department of Defense; we will make data available for other investigators after publication of the results of the primary analyses as well as the preplanned post hoc secondary analyses.