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Glucose intolerance associated with hypoxia in people living at high altitudes in the Tibetan highland
  1. Kiyohito Okumiya1,2,
  2. Ryota Sakamoto2,3,
  3. Yasuko Ishimoto4,
  4. Yumi Kimura2,
  5. Eriko Fukutomi2,
  6. Motonao Ishikawa5,
  7. Kuniaki Suwa5,
  8. Hissei Imai6,
  9. Wenling Chen2,
  10. Emiko Kato2,
  11. Masahiro Nakatsuka7,
  12. Yoriko Kasahara8,
  13. Michiko Fujisawa2,
  14. Taizo Wada2,
  15. Hongxin Wang9,
  16. Qingxiang Dai9,
  17. Huining Xu9,
  18. Haisheng Qiao10,
  19. Ri-Li Ge11,
  20. Tsering Norboo12,
  21. Norboo Tsering12,
  22. Yasuyuki Kosaka13,
  23. Mitsuhiro Nose2,
  24. Takayoshi Yamaguchi14,
  25. Toshihiro Tsukihara15,
  26. Kazuo Ando2,
  27. Tetsuya Inamura16,
  28. Shinya Takeda13,
  29. Masayuki Ishine17,
  30. Kuniaki Otsuka5,18,
  31. Kozo Matsubayashi2
  1. 1Research Department, Research Institute for Humanity and Nature, Kyoto, Japan
  2. 2Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan
  3. 3Hakubi Center for Advanced Research, Kyoto University, Kyoto, Japan
  4. 4Faculty of Medicine, Public Health Nursing, School of Nursing, Mie University, Tsu, Mie, Japan
  5. 5Department of Medicine, Tokyo Women's Medical University, Medical Center East, Tokyo, Japan
  6. 6Health Promotion and Human Behavior, Kyoto University Graduate School of Medicine, Kyoto, Japan
  7. 7Sakakibara Hakuho Hospital, Tsu, Mie, Japan
  8. 8College of Nursing, Kanto Gakuin University, Yokohama, Japan
  9. 9Affiliated Hospital of Qinghai University, Xining, Qinghai, China
  10. 10Qinghai Academy of Animal and Veterinary Sciences, Xining, Qinghai, China
  11. 11Research Center for High Altitude Medicine, Qinghai University, Xining, Qinghai, China
  12. 12Ladakh Institute of Prevention, Ladakh, India
  13. 13Graduate School of Asian and African Area Studies, Kyoto University, Kyoto, Japan
  14. 14National Institute for Agro-Environmental Sciences, Ibaragi, Japan
  15. 15Faculty of Education and Regional Studies, University of Fukui, Fukui, Japan
  16. 16The Open University of Japan, Chiba, Japan
  17. 17Yasugi Clinic, Shimane, Japan
  18. 18Chronomics & Gerontology, Tokyo Women's Medical University, Tokyo, Japan
  1. Correspondence to Dr Kiyohito Okumiya; okumiyak{at}hotmail.com

Abstract

Objectives To clarify the association between glucose intolerance and high altitudes (2900–4800 m) in a hypoxic environment in Tibetan highlanders and to verify the hypothesis that high altitude dwelling increases vulnerability to diabetes mellitus (DM) accelerated by lifestyle change or ageing.

Design Cross-sectional epidemiological study on Tibetan highlanders.

Participants We enrolled 1258 participants aged 40–87 years. The rural population comprised farmers in Domkhar (altitude 2900–3800 m) and nomads in Haiyan (3000–3100 m), Ryuho (4400 m) and Changthang (4300–4800 m). Urban area participants were from Leh (3300 m) and Jiegu (3700 m).

Main outcome measure Participants were classified into six glucose tolerance-based groups: DM, intermediate hyperglycaemia (IHG), normoglycaemia (NG), fasting DM, fasting IHG and fasting NG. Prevalence of glucose intolerance was compared in farmers, nomads and urban dwellers. Effects of dwelling at high altitude or hypoxia on glucose intolerance were analysed with the confounding factors of age, sex, obesity, lipids, haemoglobin, hypertension and lifestyle, using multiple logistic regression.

Results The prevalence of DM (fasting DM)/IHG (fasting IHG) was 8.9% (6.5%)/25.1% (12.7%), respectively, in all participants. This prevalence was higher in urban dwellers (9.5% (7.1%)/28.5% (11.7%)) and in farmers (8.5% (6.1%)/28.5% (18.3%)) compared with nomads (8.2% (5.7%)/15.7% (9.7%)) (p=0.0140/0.0001). Dwelling at high altitude was significantly associated with fasting IHG+fasting DM/fasting DM (ORs for >4500 and 3500–4499 m were 3.59/4.36 and 2.07/1.76 vs <3500 m, respectively). After adjusting for lifestyle change, hypoxaemia and polycythaemia were closely associated with glucose intolerance.

Conclusions Socioeconomic factors, hypoxaemia and the effects of altitudes >3500 m play a major role in the high prevalence of glucose intolerance in highlanders. Tibetan highlanders may be vulnerable to glucose intolerance, with polycythaemia as a sign of poor hypoxic adaptation, accelerated by lifestyle change and ageing.

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Strengths and limitations of this study

  • The study showed the first evidence of a close association of glucose intolerance with dwelling at high altitude over 3500 m or hypoxaemia after adjustment of lifestyle-related factors.

  • The study also showed the vulnerability of glucose intolerance in high-altitude people with adaptation to hypoxia accelerated by ageing and lifestyle change. In addition, this study used validated POC (point-of-care) analysers for blood glucose and haemoglobin measurement that were not affected by the high-altitude environment.

  • Farmers and nomads could be analysed in different altitudes as almost homogeneous subjects. But urban dwellers, who had changed to a modernised lifestyle, were analysed at different altitudes, which was not homogeneous. Multiple logistic regression analysis was used for adjusting lifestyle and other confounding factors.

  • It is a cross-sectional study and, thus, requires a future longitudinal study to disclose any causal relations. Also, the genes involved in adaptation to hypoxia were not examined. The study did not investigate whether nutrition and physical activities were confounding factors to glucose intolerance, but measuring body mass index and dyslipidaemia may reflect those effects.

Introduction

Over many generations, people living at high altitudes have developed unique practices to survive in challenging environments with limited ecological resources.1–3 Traditionally, diabetes mellitus (DM) has been uncommon among highlanders4 ,5 compared with lowlanders.6 ,7 Lifestyle-related diseases, such as DM and hypertension, are rapidly increasing with an increase in longevity and changes in lifestyle worldwide. A remarkable increase in DM has been reported in lowlander and semi-high altitude (around 1300 m) migrants who moved from traditional lifestyles to Westernised lifestyles.8–10 Prevention of DM has become an urgent issue among lowlanders, especially in developing countries, where the rate of DM prevalence is much faster than in developed countries.11 ,12 Older people with a low economic status in rural areas might be vulnerable to impaired glucose tolerance or DM in developed and developing countries.13 ,14 An adverse intrauterine environment is a risk factor of diabetes,15 and there is an association between low birth weight and type 2 diabetes.16 These phenomena all show adaptation to a low-caloric intake, and the rapid change to a high intake likely increases the risk of diabetes, possibly through a mechanism related to epigenetics.17 ,18

People living at high altitudes not only have the previously mentioned risk factors, but they are also subject to hypoxia in a severe natural environment.19 High-altitude dwellers show long-term adaptation to harsh environment-induced hypoxia. Highlanders are biologically adapted to hypoxic environments by various genetic mechanisms, such as an increase in haemoglobin concentrations or increased blood flow without polycythaemia.20 ,21 Andean people have increased haemoglobin levels as a result of hypoxic adaptation, but they suffer from chronic mountain sickness with excessive polycythaemia as a maladaptation to hypoxia more often than Tibetan people do.22 Ageing, menopause, respiratory disorders, obesity and hypertension are risk factors of chronic mountain sickness.23–26 As a result of rapid lifestyle changes related to urbanisation, highlanders are experiencing an alarming increase in diabetes.27–30 Tibetan residents, especially those who are obese, may be more vulnerable to glucose intolerance31 compared with Andean people.5 ,32 Whether high-altitude dwellers are more vulnerable to diabetes as a result of lifestyle changes, compared with lowlanders, is unknown, but people living at high altitudes are known to be vulnerable to hypertension.4 ,33–36

A population-based study of the effects of high altitude, between 1200 and 3000 m, on the same ethnic group, showed a low prevalence of impaired glucose regulation at high altitudes.37 Also, an inverse association has been shown between diabetes and altitudes lower than 3500 m in lowlanders.38 Additionally, lowland patients with diabetes show better glucose intolerance improvement at a mildly high altitude.39 However, there are no reports on whether dwelling at altitudes over 3500 m and hypoxaemia increases the risk of diabetes. We previously reported a strong association between glucose intolerance and polycythaemia in elderly Tibetan people living in two highland areas.40 Because they had adapted to hypoxia by reducing polycythaemia, polycythaemia may be regarded as a sign of maladaptation to hypoxia for Tibetan people.

The aim of this study was to clarify the association of glucose intolerance with hypoxaemia or dwelling at altitudes of 2900–4800 m in Tibetan highlanders, and to verify the hypothesis that high-altitude dwelling in Tibetan highlanders increases their vulnerability to DM when accelerated by lifestyle change or ageing. This hypothesis is based both on the current accelerated and modernised lifestyle change occurring in middle-aged and elderly highlanders coming from a traditional childhood lifestyle, and on the high prevalence of polycythaemia in elderly Tibetan highlanders in contrast with younger Tibetans.

Methods

Study population

This cross-sectional epidemiological study was carried out from 2008 to 2011. A total of 1258 participants aged 40–87 years (mean age±SD, 58.0±11.5 years) were examined. The study population consisted of consecutive volunteers who attended our medical camps held in three highland communities in China as follows: 86 Tibetan nomads (mean age, 66.7±5.3 years; male/female: 40/46) living in a Tibetan nomadic village in Haiyan County (altitude, 3000–3100 m) in 2008; 324 Tibetan urban residents (59.3±10.6 years; 127/197) in Jiegu Town (3700 m) in 2009; and 41 Tibetan nomads in Ryuho (4200–4400 m) in 2011 in Yushu County, Qinghai (table 1).

Table 1

Characteristics of the field sites in high-altitude regions

Participants in three highland communities in Ladakh, India, were examined as follows: 295 Ladakhi farmers (57.3±12.0 years, 113/182) in Domkhar village (2900–3800 m) in 2009; 308 urban residents (42 Ladakhi/266 Tibetan) (58.1±12.2 years, 126/182) in Leh Town (3300 m) in 2010; and 204 nomads (126 Ladakhi/78 Tibetan) (54.4±10.9 years, 113/91) in six villages in Changthang (4300–4800 m) in 2011. The participants consisted of people aged 40 years or older, except for 86 participants in Haiyan who were elderly people aged 60 years or older. Announcements for recruitment were carried out by health staff of Qinghai University and health centres in a nomadic village in Haiyan, and in two villages in Ryuho and Jiegu Town. Announcements were also made to people aged 40 years or older by health staff from Ladakh Institute of Prevention, from health centres and from village leaders in the three villages in Domkhar, four villages in Changthang and five colonies of migrants in Leh. We carried out health checks of the volunteer participants in health centres or community halls in rural villages or towns.

Haiyan County (population, 38 000) contains a Tibetan nomadic village with 465 people aged 60 years or older,41 ,42 who were all invited as volunteer participants for medical check-ups. Eighty-six agreed to take part. The study site in Haiyan County was located between an agricultural area inhabited by Han Chinese and a pastoral area inhabited by native Tibetans.43

Ryuho consists of six villages with a total population of 8700, including 1604 residents aged 40 years or older.44 People in two representative villages were invited to participate in medical check-ups and 41 Tibetan nomads agreed. Their lifestyle is difficult because of the high altitude and severe cold. They move from one pasture (altitude approximately 4100 m) to another (approximately 5000 m) and back every 6 months, along with their livestock.

Jiegu Town, with a population of 23 000, is the seat of Yushu County (population, 67 000) in a nomadic area of Qinghai on the Tibetan plateau. With socioeconomic globalisation, this town is a rapidly developing area (population in 1996, 6460).45 ,46 People aged 40 years or older were randomly invited for medical check-ups and 324 of the urban Tibetan residents agreed to participate. These participants had varied occupations, which included being active or retired nomads, farmers or official workers. They were considered to have a lifestyle similar to city dwellers.

Domkhar in Ladakh is a rural village along the Domkhar valley, which consists of the three communities of Dho, Barma and Gongma, at altitudes of 2900, 3400 and 3800 m, respectively, with a total population of 1269 people, including 449 aged 40 years or older.47 All were invited for medical check-ups, of whom 295 Ladakhi farmers agreed to volunteer as participants in the survey. The area was not easily accessible and Domkhar has only begun to experience the effects of socioeconomic globalisation in the past few years. Most residents were active farmers with traditional lifestyles, who had experienced changes in their food habits and received access to electricity.

Since the 1970s, the residents of Leh Town have had a history of migration from the Changthang area; they comprise Tibetan as well as Ladakhi nomads. From four representative colonies (population 1435), 637 people aged 40 years or older were invited as volunteer participants for medical check-ups, and 308 of these urban residents (266 Tibetans and 42 Ladakhis) agreed to participate in the survey. They were included in the urban population group because they were considered to have adopted a lifestyle similar to that of city dwellers.

Changthang is the highest plateau (altitude 4300–4800 m) among the regions included in this study. From five representative villages (total population 1840), 491 people aged 40 years or older were invited for medical check-ups and 204 nomads (78 Tibetans and 126 Ladakhis) voluntarily agreed to participate. This population is generally made up of nomads who move with their livestock from pasture to pasture every 3 months. Their lifestyle is difficult because of the high altitude and severe cold.

Measurements

Anthropometric measurements, including weight and height, were obtained using standard techniques. Body mass index (BMI) was calculated as weight (kg)/height (m2). Blood pressure was measured on the arm using an automatic device (HEM 7000; Omron Life Science Co Ltd, Kyoto, Japan) based on the cuff oscillometric principle, and its accuracy has been validated in previous studies.48 Oxyhaemoglobin saturation (SpO2) was measured by a pulse oximeter (PULSOX-300; Konica Minolta Co Ltd, Tokyo, Japan). Blood pressure and SpO2 were measured twice, in the sitting position, after taking a 5 min rest, at least, and the mean systolic blood pressure (SBP), diastolic blood pressure (DBP) and SpO2 were calculated. Hypertension was defined as SBP ≥140 mm Hg and/or DBP of ≥90 mm Hg and/or taking current antihypertensive medicine.49

Overnight fasting venous samples were collected in the morning from all participants. Blood glucose was analysed in venous whole blood by a new-generation StatStrip analyser (Nova Biomedical, Boston, Massachusetts, USA), which has been validated as being affected by neither haematocrit50–52 nor high altitude.53 Additionally, the StatStrip is as accurate for measuring plasma glucose as a standard laboratory analyser is,50–52 although previous analysers were influenced by haematocrit50–52 and high altitude.54 Haemoglobin (Hb) was analysed by the Hemocue haemoglobinometer (Hemocue, Angelholm, Sweden).55 ,56 Samples were analysed at 15–25°C. Lipids were analysed by either a central laboratory in Qinghai University Hospital, China, or at SRL Inc, India.

Stages of glucose tolerance were classified into either normoglycaemia (NG) or hyperglycaemia (HG) by an oral glucose tolerance test (OGTT) according to WHO criteria. The presence of HG consisting of DM and intermediate HG (IHG) was defined as follows: DM was fasting blood glucose (FBG) levels ≥7.0 mmol/L or 2 h blood glucose levels of ≥11.1 mmol/L, and IHG was fasting glucose levels ≥6.1 mmol/L and <7.0 mmol/L or 2 h glucose levels ≥7.8 mmol/L and <11.1 mmol/L. Fasting NG and fasting HG were defined using only FBG results. Fasting HG consisted of fasting IHG (FBG levels ≥6.1 and <7.0 mmol/L) and fasting DM (FBG levels ≥7.0 mmol/L).57

Overweight was defined as a BMI of 25 or over. Dyslipidaemia was defined as triglycerides >150 mg/dL, total cholesterol >220 mg/dL and/or high-density lipoprotein (HDL) cholesterol <40 mg/dL. Hypoxaemia was defined as SpO2 <89 mm Hg. Levels of Hb were classified into four groups: anaemia (Hb <13 g/dL for males and <12 g/dL for females), normal (Hb <18 and ≥13 g/dL for males and Hb <16 and ≥12 g/dL for females), moderate polycythaemia (Hb <21 and ≥18 g/dL for males and Hb <19 and ≥16 g/dL for females) and excessive polycythaemia (Hb ≥21 g/dL for males and ≥19 g/dL for females).

Levels of glycated Hb (HbA1c) based on the National Glycohemoglobin Standardization Program (NGSP) were analysed by Latex agglutination immunoassay (DCA 2000 HbA1c, Siemens Healthcare Co Ltd, Munich, Germany) in 949 participants in all the field sites except for participants in Haiyan. The frequencies of HbA1c levels ≥6.0% and ≥6.5% were compared among the different classifications of glucose intolerance.

The age of the participants was confirmed with reference to carefully prepared cross-tabulation correlating their date of birth with the animal year, which the rural population always remembered, and with historical sentinel events in the case of elderly participants. The interviewers asked participants whether they currently or previously smoked or currently drank alcohol.

Statistics

The χ2 test, Student t test and one-way analysis of variance (ANOVA) were conducted to analyse: the rate of prevalence of glucose intolerance, hypertension, overweight, hypoxaemia, dyslipidaemia, anaemia and polycythaemia; altitude level, dwelling area and livelihood; mean blood glucose, SBP, DBP, BMI, SpO2, lipids and Hb levels. The associations of glucose intolerance with the confounding factors, including altitude, hypoxaemia, age, sex, overweight, dyslipidaemia and livelihood, were analysed by multiple logistic regression analysis. Analyses were performed using SPSS V.17.0 (SPSS Inc, Chicago, Illinois, USA). A p value <0.05 was considered statistically significant.

Results

The characteristics of all of the studied variables and the differences by sex are shown in table 2. Male participants were older with a lower prevalence of overweight than female participants. They also had a higher prevalence of IHG/fasting IHG, DM/fasting DM, high triglyceride levels, low HDL levels and dyslipidaemia, excessive polycythaemia and a lower prevalence of anaemia than female participants. In addition, male participants were more prevalent at altitudes over 4500 m and as nomads than female participants, but females were more prevalent as farmers and urban dwellers. The prevalence of current or past smokers was 4.7% (males 9.4% and females 1.1%). The prevalence of current alcohol drinkers was 25.4% (males 35.7% and females 17.6%).

Table 2

Characteristics of the studied variables by sex

The characteristics of all the studied variables by difference in age are shown in table 3. Male participants were more prevalent at age 70 years or older. The prevalence of dyslipidaemia and hypertension was higher in older participants. Older people were also more prevalent as urban dwellers, but less prevalent in dwelling over 4500 m. Prevalence of hypoxaemia (SpO2 <89%) was higher in older participants, but not for anaemia and excessive polycythaemia.

Table 3

Characteristics of the studied variables by age

Table 4 shows the association of glucose intolerance (fasting IHG/fasting DM or IHG/DM), using the criteria of FBG and OGTT, with all of the studied variables. Glucose intolerance increased with age. Glucose intolerance was also associated with male sex, overweight, dyslipidaemia, hypertension, hypoxaemia, high Hb levels, high HbA1c levels, high altitude and urban dwellers/farmers (vs nomads). Neither smoking nor drinking alcohol was associated with glucose intolerance.

Table 4

Variables associated with glucose intolerance

The prevalence of glucose intolerance, overweight, dyslipidaemia, hypertension, hypoxaemia and polycythaemia are shown at different altitude areas in farmers, nomads and urban dwellers (table 5). In farmers, the prevalence of fasting HG using the FBG criterion was significantly increased with elevation of altitude (2900, 3400 and 3800 m) in the three communities of Dho, Barma and Gongma (n=106 (16.0%), 74 (20.3%), 115 (34.8%), respectively, p=0.0033) in Domkhar, Ladakh. The prevalence of HG, using the OGTT criterion in the three communities, was also increased (30.2%, 36.5%, 43.5%, respectively), but not significantly. In comparison, between the two altitude levels of <3500 m (Dho and Barma) and ≥3500 m (Gongma), the prevalence of glucose intolerance using the FBG criterion was significantly increased with elevation of altitude (fasting HG: 17.8% vs 34.8%, fasting DM: 3.3% vs 10.4%, HbA1c >6.0%: 15.5% vs 29.9%) in spite of the low prevalence of overweight people dwelling at a higher altitude compared with those at a lower altitude (table 5).

Table 5

Prevalence of glucose intolerance and other variables at different altitudes, in farmers, nomads and urban dwellers

A comparison of nomad participants was conducted between Haiyan and Ryuho in Qinghai, and the prevalence of fasting HG using the FBG criterion was found to increase, but not significantly (8.1% vs 12.2%) with elevation of altitude (3000–3100 vs 4200–4400 m). In Changthang-1 and Changthang-2 in Ladakh, the prevalence of fasting HG using FBG criterion increased (12.8% vs 21.0%) with elevation of altitude (4300–4400 vs 4500–4800 m), but not significantly, while the prevalence of fasting DM significantly increased (0.0% vs 8.9%, p=0.0339). Comparing the three altitude levels, <3500 (Haiyan), 3500–4499 (Ryuho and Changthang-1) and ≥4500 m (Changthang-2), the prevalence of glucose intolerance with the FBG criterion was significantly increased with elevation of altitude (fasting HG: 8.1% (Haiyan) vs 12.5% (Ryuho and Changthang-1) vs 21.0% (Changthan-2), fasting DM: 3.5% vs 2.3% vs 8.9%) despite the low prevalence of overweight and dyslipidaemia in people dwelling at a higher altitude than at a lower altitude (table 5).

In urban dwellers, using both criteria to measure fasting HG, fasting DM, HG, DM and HbA1c, showed that the prevalence of glucose intolerance increased significantly with elevation of altitude (table 5). The results also indicated a greater prevalence of overweight and hyperlipidaemia in people dwelling at a higher altitude than in those at a lower altitude. Prevalence of hypertension was not associated with altitude levels in farmers, nomads and urban dwellers.

Prevalence of hypoxaemia and polycythaemia was significantly increased with elevation of altitude in all the groups of farmers, nomads and urban dwellers.

The association of the variables Hb, overweight and glucose intolerance, with hypoxaemia (SpO2 <89%) as the dependent variable, was separately analysed after adjustment for age, sex and altitude levels, using multiple logistic regression (models 1–3). Polycythaemia (model 1), overweight (models 2 and 3), fasting HG (model 2), fasting DM (OR=1.70, CI 1.02 to 2.85, vs no fasting DM, p=0.0413), HG (model 3) and DM (OR=1.90, CI 1.21 to 2.96, vs no DM, P=0.0049) were associated with hypoxaemia independent of altitude levels (see online supplementary table S1).

The variables that were associated with glucose intolerance and adjusted for age and sex in the four models (for dependent variables of fasting HG, fasting DM, HG and DM) by multiple logistic regression analysis are shown in table 6. Overweight (all models), hypoxaemia (all models), dyslipidaemia (all models), polycythaemia (all models), farmers (models for fasting HG and for HG, vs nomads) and urban dwellers (models for HG vs nomads) were associated with glucose intolerance after adjustment for age and sex. Altitude level of 3500–4499 m was closely associated with all models, while the highest level of ≥4500 m was associated with fasting HG and fasting DM, but not with HG or DM. By classifying the altitude level into two groups of 2500–3499 and ≥3500 m and analysing by multiple logistic regression, the altitude level of ≥3500 m was closely associated with glucose intolerance in all the models compared with 2500–3499 m altitude.

Table 6

Variables associated with glucose intolerance adjusted for age and sex by multiple logistic regression analysis

The associations of high altitude, hypoxaemia and polycythaemia with glucose intolerance and adjusted for related variables in the four models were analysed by multiple logistic regression (table 7). For the dependent variables of glucose intolerance (fasting HG/fasting DM) as measured using the FBG criterion, the results showed that dwelling at high altitudes was a closely associated factor (ORs of fasting HG/fasting DM for >4500 and 3500–3999 m were 3.59/4.36 and 2.07/1.76 vs <3500 m) independent of hypoxaemia and the lifestyle-related factors of overweight and livelihood. Classifying the altitude levels into two groups, 2500–3499 and ≥3500 m, and using multiple logistic regression analysis, the ≥3500 m altitude level was also closely associated with fasting HG (OR=2.19, CI 1.53 to 3.12, p<0.0001) and fasting DM (OR=1.94, CI 1.06 to 3.55, p=0.0305), but not with HG and not with DM. For the dependent variables of glucose intolerance (HG and DM), using the OGTT criterion, there was no association with dwelling at a high altitude, but hypoxaemia was a closely associated factor. Polycythaemia, overweight, dyslipidaemia, farmers and urban dwellers were also closely associated with glucose intolerance according to both criteria. Hypertension was not associated with glucose intolerance by multivariate analysis.

Table 7

Associations of high-altitude, hypoxaemia and polycythaemia with glucose intolerance adjusted for all other confounders by multiple logistic regression analysis

The effect of livelihood or ethnicity on the association between altitudes and fasting HG was analysed and is shown in online supplementary table S2. In models 1–3, multivariate analyses were carried out separately in each livelihood of farmer, nomad and urban dweller. In farmers and nomads, higher altitude had significantly higher OR for fasting HG compared with lower altitude, but not in urban dwellers after adjustment with other confounding factors. In models 4 and 5, multivariate analysis was carried out separately in each ethnic group, Tibetan and Ladakhi. Higher altitude had significantly greater OR compared with lower altitude in both groups. The OR was higher in the Ladakhi (2.53 vs 1.69) than in the Tibetan group. In models 6 and 7, multivariate analysis was carried out in all participants, and higher altitude had significantly greater OR compared with lower altitude independently of livelihood and ethnicity (see online supplementary table S2).

Discussion

Association of hypoxaemia with glucose intolerance

The association of glucose intolerance with lifestyle-related health factors and hypoxaemia was studied in 1258 residents dwelling at a wide range of altitudes from 2900 to 4800 m in the Tibetan highland. Previous studies have reported a negative association between glucose intolerance and altitudes below 3500 m in Tibetans37 and lowlanders.38 To the best of our knowledge, we showed, for the first time, epidemiological evidence of a positive association between the prevalence of fasting glucose intolerance at high altitudes over 3500 m and independently of hypoxaemia. These positive effects of dwelling at high altitudes were more distinct when glucose intolerance was defined by FBG. The association of high altitude ≥3500 m with fasting HG was shown even after adjustment of the effects of both lifestyle and ethnicity in all participants, and also in a separate analysis of farmers and nomads, but not in urban dwellers. Analysis of hypoxaemia, using SpO2, was closely associated with a high prevalence of glucose intolerance as measured by FBG and OGTT after adjustment for both dwelling at high altitude and all lifestyle-related health factors (figure 1).

Figure 1

Hypothesised associations of hypoxaemia, polycythaemia and glucose intolerance with the influence of ageing and lifestyle change. Hypothesis 1 (*1 and *2) and hypothesis 2 (*3 and *4) in the association of hypoxia, polycythaemia and glucose intolerance. Ageing and lifestyle change accelerate the association, ‘Diabetes acceleration hypothesis’. *1 or 4: Proposed according to online supplementary table S1 and table 5. *2 or 3: Proposed according to data in tables 6 and 7, online supplementary table S1, and previous studies.58–64 *1 and *2: Proposed according to a previous study.66 *3 and *4: Proposed according to previous studies.76 ,77 *5: Proposed according to table 3. *6: Proposed according to our data compared with a previous study.74 *7: Proposed according to online supplementary table S1.

Comparison with other studies on the association between hypoxaemia and glucose intolerance

No previous reports have shown an association between hypoxia and glucose intolerance in high-altitude dwellers. However, there are some reports on the association of hypoxaemia with glucose intolerance in lowlanders58–63 and in animals.64 People with respiratory diseases,58 sleep apnoea syndrome60 ,61 and insomnia,62 ,63 have a high prevalence of glucose intolerance. An isolated high fasting glucose pattern in a glucose tolerance test was dominant and insulin resistance increased in these people.60–63 High-altitude dwellers have more severe hypoxaemia during sleep than during waking hours, and the prevalence of insomnia was higher in people dwelling at higher altitudes. Aggravation of insulin resistance in an acute hypoxic environment has also been reported to be caused not only by an increase in stress hormones, including epinephrine and corticosteroid, but also by the direct effect of hypoxia.59 Although there are no reports on humans showing vulnerability to glucose intolerance in newborns from mothers who have hypoxaemia, this has been reported in calves (figure 1).64

In our previous study on lowlanders aged 40 years or older in Tosa, Japan, the prevalence of HG (fasting HG) and DM (fasting DM) was 46.2% (22.7%) and 16.7% (6.8%), respectively.65 In that study, the percentages of ‘fasting HG in HG’ (‘fasting DM in DM’) were 49.1% (40.7%). Those percentages were calculated using the same data as in table 5 and they increased parallel with altitude (2500–3499 vs 3500–4499 vs 4500 m). In farmers, the percentages of ‘fasting HG in HG’ (‘fasting DM in DM’) increased with elevation of altitude from 54.3% (45.8%) to 80.0% (100%), in nomads these percentages also increased from 38.8% (43.2%) to 50.0% (40.4%) to 84.7% (92.7%), and in urban dwellers, these percentages increased from 30.8% (59.2%) to 61.1% (79.9%). The percentage of ‘fasting HG in HG’ in farmers and urban dwellers exceeded 60–80% above 3500 m, and in nomads, exceeded 80% above 4500 m. The 2 h-BG levels in our result in fasting IHG/fasting DM were much lower (130.7/224.5 mg/dL) compared with those in Tosa Town (172.4/ 262.6 mg/dL), though FBG levels in our study of fasting IHG/fasting DM (115.2/166.0 mg/dL) were not lower compared with those in Tosa Town (115.8/146.2 mg/dL). These results are compatible with the isolated high fasting glucose pattern and increased insulin resistance in people who have hypoxaemia and stress while sleeping.60–63

Our results and previous reports suggest that mild hypoxia and a higher metabolic rate in participants living at an altitude lower than 3500 m may be preventive for glucose intolerance. However, moderate hypoxia and dwelling at higher altitudes over 3500 m may increase the risk of fasting glucose intolerance (figure 1).

Hypothesis of associations of polycythaemia, hypoxaemia and glucose intolerance in high-altitude dwellers

An association between polycythaemia and glucose intolerance in a small sample population has been previously reported.40 In our study of a large high-altitude population, using multivariate analysis, after adjustment for lifestyle-related factors and hypoxaemia, this association was strong. As a result, we propose the following two hypotheses to explain the mechanism of the strong associations among polycythaemia, hypoxaemia and glucose intolerance. The first hypothesis is that polycythaemia aggravates hypoxaemia and leads to glucose intolerance (*1 and *2 in figure 1). This hypothesis was based on the association of polycythaemia with hypoxaemia (table 5 and online supplementary table S1) and the association of hypoxaemia with glucose intolerance (tables 6 and 7).

The cross-sectional design of our study is a limitation to the causal relation. Additionally, there are no longitudinal studies on highlanders. However, a previous longitudinal report on lowlanders in a large 9-year follow-up study supports our hypothesis in which the highest haematocrit group (>44.3%) has a higher risk (OR=1.6) of diabetes onset compared with the lowest group (<39.0%).66 The mechanism of this association is speculated as follows: lower blood circulation caused by the high viscosity of polycythaemia is considered to disrupt the transport of insulin, glucose and oxygen to tissue cells, resulting in low cellular intake of glucose and lower activity of respiration, which requires oxygen and glucose in cells.66 Our study showed an association of anaemia with HG.67 This supports our first hypothesis because anaemia results in low activity of O2 transport and subsequent hypoxaemia in tissue, which is aggravated at high altitude. Almost all study participants with anaemia had iron deficiency.67 Also, Ladakhi farmers eat less meat than Tibetan nomads do,33 ,68 and are vulnerable to suffering from iron deficiency anaemia.

Polycythaemia may be a sign of maladaptation to hypoxia, which leads to glucose intolerance, because Tibetan people have acquired a hypoxic adaptation by preventing polycythaemia through a genetic response to hypoxia.20 ,21 ,69–73 Tibetans (mean age 34–35 years, BMI 18.4–18.5) compared with Andean highlanders (mean age 37–38 years, BMI 22.3–23.4) living at similar altitudes (4000 m) have lower Hb levels (male/female: 15.6/14.2 vs 19.1/17.8 g/dL, respectively), though participants with anaemia were excluded from this study.74 The genes responsible for lower Hb are PPARA, EGLN1 and EPAS1, and the mean Hb of both sexes is decreased by these advantageous haplotypes.70 The frequency of the adaptive haplotype of EPAS1 is reported to be 87% in Tibetans and 9% in Han Chinese.71 The sex-adjusted Hb level is 0.8 g/dL lower in homozygotes compared with heterozygotes of EPAS1.69

Mean Hb in our older participants (mean 58.3 years, BMI 24.0–25.1, altitude 2900–4800 m, anaemia was excluded) was 16.1 g/dL (male/female: 17.2/15.1), which was 1.6/0.9 g/dL higher compared with the younger Tibetans (34–35 years).74 Excluding overweight participants from the older group, the mean Hb of these older participants was 1.4/0.6 g/dL (male/female) higher compared with that of the younger participants.74 Another report showed the associations between age, polycythaemia and the genes that have adapted to hypoxia. Older Tibetan chronic mountain sickness patients (mean age 54 years, n=45) have as much as 96% of the adaptive haplotype EPAS1 compared with 72% in younger people without polycythaemia (mean age: 30 years, n=34).75 Ageing may aggravate not only hypoxaemia (table 3) but also polycythaemia in Tibetan people (*5 and *6 in figure 1), even in those with adaptive haplotypes.75

Hypothesis of associations of glucose intolerance, hypoxaemia and polycythaemia in high-altitude dwellers

Our second hypothesis is that glucose intolerance leads to microvascular complications and aggravation of tissue hypoxaemia. Polycythaemia is enhanced with compensation for this hypoxaemia (*3 and *4 in figure 1). The basis of our results for this hypothesis is the same as in the first hypothesis because it was a cross-sectional study without causal relation. However, there are observational studies in lowlanders supporting this hypothesis.76 ,77 Infants have complications of polycythaemia from mothers with diabetes. Chronic intrauterine hypoxaemia intermediates this association.76 ,77

The advantageous homozygotes of the genes that are involved in adaptation to hypoxia (EGLN1 and PPARA) have stronger metabolic effects to facilitate anaerobic glycolysis and regression of lipid catabolism, resulting in higher serum concentrations of lipids by adaptation to hypoxia.70 ,78 ,79 This effect may prevent the development of diabetes in younger people with a traditional lifestyle. However, sedentary workers and elderly people with advantageous genetic adaptations to hypoxia may be vulnerable to obesity, hyperlipidaemia and increased insulin resistance by lifestyle changes compared with people without these advantageous genes. Obesity and dyslipidaemia lead not only to increased insulin resistance (tables 6 and 7), but also to hypoxaemia (see online supplementary table S1 and *7 in figure 1).

Higher Hb was found in participants in Jiegu Town who were overweight compared with those not overweight. This difference in Hb (male/female 1.3/0.9 g/dL) was higher than the difference of the sex-adjusted Hb (0.8 g/dL) in homozygotes compared with heterozygotes of EPAS1.69 The effect of lifestyle changes on polycythaemia in our study overcame the acquired hypoxic adaptation for prevention of polycythaemia. People in Jiegu Town (3700 m) with a high prevalence of overweight also had the highest prevalence of HG and fasting HG. Those parameters were accelerated both by lifestyle changes and by hypoxaemia, with an extremely high frequency of polycythaemia. While people in the higher altitude community of Domkhar (3800 m) had a low prevalence of overweight, they had a high prevalence of HG with an especially high fasting HG and polycythaemia, which may be accelerated mainly by hypoxia.

Our first hypothesis mainly explains our result of the association of dwelling at high altitude or hypoxaemia with an increase in glucose intolerance as seen in Domkhar. The second hypothesis is mainly associated with our result of lifestyle-related factors leading to an increase in glucose intolerance. According to our results and previous reports, these two hypotheses may be acting simultaneously with each other, considering the close associations among hypoxaemia, polycythaemia and glucose intolerance as seen in Jiegu Town. The association of glucose intolerance with polycythaemia and hypoxaemia is accelerated by ageing and lifestyle changes, and there may be the vulnerability of the hypoxia adaptive genes for glucose intolerance as the background, called the ‘Diabetes acceleration hypothesis’ (figure 1).

Conclusions, unanswered questions and policy implications

Socioeconomic factors and hypoxia—the effect of altitudes over 3500 m—play major roles in the prevalence of glucose intolerance in highlanders. Hypoxaemia and polycythaemia were closely associated with glucose intolerance after adjustment for the effects of lifestyle changes in our study. Tibetan people may be vulnerable to glucose intolerance, with polycythaemia as a sign of poor hypoxic adaptation. However, the mechanism of the onset of glucose intolerance by hypoxaemia in high-altitude dwellers is unknown. A previous report showed higher oxidative stress in Tibetan people compared with in Han people, and higher oxidative stress was associated with glucose intolerance and arteriosclerosis.80 ,81 Further examination of the metabolic mechanism and oxidative stress in association with the effects of genes involved in hypoxia adaptation in high-altitude dwellers is needed. Because the matter of whether the WHO criteria of glucose intolerance can be applicable in high-altitude people has not been established, the prognosis of fasting HG and HG associated with high-altitude should be examined longitudinally. Prevention of lifestyle-related diseases and health education should be advocated, especially in high altitude dwellers, with rapidly prevailing socioeconomic globalisation.

Acknowledgments

The authors thank the participants and staff of Qinghai University Affiliated Hospital, the People's Hospital of Haiyan and Yushu County, Qinghai, China, Ladakh Institute of Prevention and Health centres in Domkhar and Changthang, and the High-altitude project of Research Institute for Humanity and Nature, Kyoto, Japan. They thank Airong Yang, Zhanquan Li and Haiying Tong (Qinghai University Affiliated Hospital) and also thank Tsering Motup, Rigzin Dolma, Ishey Lhamo, Sherab Dolma, Rinchen Dolma and Kunznag Dolma (Ladakh Institute of Prevention). The authors thank Naomune Yamamoto (Aino Hospital, Osaka, Japan), Shun Nakajima and Reiko Hozo (Tokyo Women's Medical University, Medical Center East, Tokyo, Japan). They acknowledge Yukiko Kita and Chizu Wada for their assistance.

References

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Footnotes

  • Contributors KO, RS, R-LG, TN, TT, KA, TI, ST, MI, KO and KM were involved in the study conception and design. KO obtained funding. KO, RS,YI, YK, EF, MI, KS, HI, WC, EK, MN, YK, HW, QD, HX, HQ, TN, NT, YK, NM, TY, TT, KA, TI, ST, MI, KO and KM participated in the field study and acquired data. All the authors were involved in analysis and interpretation of the data. KO drafted the article; RS, MF, TW and KM revised it critically for important intellectual content; all the authors approved the final version. KO is guarantor.

  • Funding The study was funded by the High Altitude Project (leader: KO) in the Research Institute for Humanity and Nature, Kyoto, Japan (D-03, Human Life, Aging and Disease in High-Altitude Environments: Physio-Medical, Ecological and Cultural Adaptation in ‘Highland Civilisations’), from Ministry of Education, Culture, Sports, Science and Technology.

  • Disclaimer No sponsor participated in the design of the study or in the collection, analysis or interpretation of the data.

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval The study protocol was approved by the institutional review board of Research Institute for Humanity and Nature, Kyoto, Japan (RIHN-2007-02

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement No additional data are available.

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