Article Text


Glycated haemoglobin A1c is associated with low-grade albuminuria in Chinese adults
  1. Xiaolin Huang1,2,
  2. Yulin Zhou1,2,
  3. Baihui Xu1,2,
  4. Wanwan Sun1,2,
  5. Lin Lin1,2,
  6. Jichao Sun1,2,
  7. Min Xu1,2,
  8. Jieli Lu1,2,
  9. Yufang Bi1,2,
  10. Weiqing Wang1,2,
  11. Yu Xu1,2,
  12. Guang Ning1,2
  1. 1Key Laboratory for Endocrine and Metabolic Diseases of Ministry of Health, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, E-Institute of Shanghai Universities, Shanghai, China
  2. 2Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Department of Endocrine and Metabolic Diseases, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai, China
  1. Correspondence to Dr Yu Xu; jane.yuxu{at}


Background and objective Diabetes is a strong risk factor for cardiovascular diseases, whereas few studies have investigated simultaneously the associations of glycated haemoglobin A1c (HbA1c), fasting blood glucose (fasting plasma glucose (FPG)) and 2 h postload blood glucose (2 h PG) with low-grade albuminuria, which is an earlier marker of cardiovascular diseases in the general population. Our study aimed to investigate and compare associations of HbA1c, FPG, and 2 h PG levels with risks of low-grade albuminuria in the middle-aged and elderly Chinese.

Design and methods This was a cross-sectional study involving 9188 participants aged 40 years or older. All participants underwent a standard 75 g oral glucose tolerance test. Low-grade albuminuria was defined as the highest quartile of urinary albumin-to-creatinine ratio (ACR) (>6.10 mg/g in males and >8.76 mg/g in females) in respondents without microalbuminuria or macroalbuminuria.

Results HbA1c, FPG and 2 h PG were all significantly correlated with urinary ACR after adjustment for confounders (all p values <0.0001). After adjustment for HbA1c, the relationships of FPG and 2 h PG with ACR reduced to null. HbA1c levels were still significantly associated with ACR after further adjustment for FPG and 2 h PG. Multiple logistic regression showed that risks of low-grade albuminuria were positively associated with HbA1c levels in a dose–response manner. Compared with participants with HbA1c ≤37 mmol/mol (5.5%), ORs (95% CIs) for low-grade albuminuria were 1.05 (0.94 to 1.18), 1.25 (1.04 to 1.50), 1.40 (1.04 to 1.90) and 2.21 (1.61 to 3.03) for HbA1c categories of 38–42 mmol/mol (5.6–6.0%), 43–48 mmol/mol (6.1–6.5%), 49–53 mmol/mol (6.6–7.0%), and >53 mmol/mol (7.0%), respectively (pfor trend <0.0001).

Conclusions HbA1c, but not FPG or 2 h PG, was independently associated with an increased risk of low-grade albuminuria in the middle-aged and elderly Chinese.

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Strengths and limitations of this study

  • The current study is a large community-based study in a general Chinese population, which explored the association between three glycaemic indicators and low-grade albuminuria.

  • This might be the first study to compare the associations among glycated haemoglobin A1c (HbA1c), fasting plasma glucose and 2 h postload glucose (2 h PG) with low-grade albuminuria.

  • The cross-sectional nature of this study limited us to conclude a causal relationship between HbA1c and low-grade albuminuria.

  • Urinary albumin excretion assessed from a single urine sample was also an important limitation of this study.


The presence of microalbuminuria, which is defined as urinary albumin-to-creatinine ratio (ACR) between 30 mg/g and 299 mg/g, predicts the progression of chronic kidney disease or nephropathy in patients with diabetes.1 In addition, it is well recognised that microalbuminuria is an important risk factor for cardiovascular diseases.2–5 However, accumulating evidence has shown recently that low-grade albuminuria, an earlier stage with urinary ACR below the microalbuminuria threshold, is also associated with increased risk of cardiovascular morbidity and mortality.6–8 The Heart Outcomes Prevention Evaluation found a continuous association between levels of urinary ACR and cardiovascular events. Major cardiovascular events increased by 5.9% for every 3.0 mg/g increase in urinary ACR, starting at the urinary ACR level of 4.4 mg/g.2

Diabetes is a strong, independent risk factor for cardiovascular diseases. The relative risk of cardiovascular diseases in those with diabetes is approximately twice the level seen in those without diabetes.9 Although glycated haemoglobin A1c (HbA1c), which was integrated into the diagnostic criteria for diabetes by the American Diabetes Association in 2010, has been demonstrated to be associated with cardiovascular diseases in observational studies,10 evidence from randomised controlled trials is not consistent regarding HbA1c reduction and macrovascular outcomes.11 ,12 Moreover, few studies have examined the association between HbA1c concentrations and low-grade albuminuria, an early marker of cardiovascular diseases, or compared the associations with low-grade albuminuria among HbA1c, fasting plasma glucose (FPG) and 2 h postload glucose (2 h PG).

Hence, this study examined the association between low-grade albuminuria and HbA1c with comparisons to FPG and 2 h PG in the middle-aged and elderly Chinese.

Materials and methods

Study population

This was a community-based cross-sectional study, which recruited residents aged 40 years or older from Jiading District, Shanghai, China from March to August 2010. The study design and protocol were described previously.13 ,14 A total of 10 375 respondents participated in this survey. The prevalence of microalbuminuria (ACR 30–299 mg/g) was 6.3% and that of macroalbuminuria (ACR ≥300 mg/g) was 0.5%. We excluded participants with self-reported histories of renal diseases including glomerulonephritis, nephritic syndrome, malignancy, gouty nephropathy and lupus nephritis (n=492); participants with estimated glomerular filtration rate (eGFR) <15 mL/min/1.73 m2 (n=5); participants with missing information on FPG, 2 h PG, HbA1c or urinary ACR (n=69); and participants with microalbuminuria (n=580) or macroalbuminuria (n=41). Therefore, a total of 9188 participants were included in the current analysis.

Data collection

Detailed information on sociodemographics, medical history and lifestyle was acquired using a standard questionnaire completed by trained physicians using a face-to-face interview. Current smoker was defined as someone who smoked one cigarette per day or seven cigarettes per week in the past 6 months. Current drinker was defined as someone who consumed alcohol once a week over the past 6 months. Levels of physical activity including leisure time and work-related physical activity were categorised as low, moderate and high, based on the short form of the International Physical Activity Questionnaire (IPAQ).15

Anthropometric characteristics were measured by trained personnel according to a standard protocol. Height and weight were measured without shoes and in lightweight clothing to the nearest 0.1 cm and 0.1 kg, respectively. Body mass index (BMI) was calculated by weight (kilograms) divided by squared height (metres). Obesity was defined as a BMI≥30 kg/m2.16 Blood pressure was measured on the non-dominant arm by an automated electronic device (OMRON Model HEM-752, FUZZY, Omron Company, Dalian, China) three times with a 1 min interval in a sitting position after 5 min rest. Three readings were averaged for analysis. Hypertension was defined by systolic blood pressure (SBP) ≥140 mm Hg and/or diastolic blood pressure (DBP) ≥90 mm Hg and/or use of antihypertensive medications.

Biochemical evaluation

Venous blood samples were collected following an overnight fast of at least 10 h. A standard 75 g oral glucose tolerance test (OGTT) was performed and 2 h PG samples were also collected. Blood glucose levels were measured using the glucose oxidase method on an autoanalyzer (Modular P800; Roche, Basel, Switzerland). Total cholesterol, low-density lipoprotein cholesterol (LDL-c), high-density lipoprotein cholesterol (HDL-c), triglycerides (TG) and serum creatinine were also measured on the autoanalyzer (Modular E170; Roche, Basel, Switzerland). HbA1c was determined by high-performance liquid chromatography (BIO-RAD D-10, USA). Diabetes was diagnosed according to the 1999 WHO criteria, that is, an FPG ≥7.0 mmol/L, and/or 2 h PG ≥11.1 mmol/L, and/or a self-reported previous diagnosis by physicians. The index of homoeostasis model assessment of insulin resistance (HOMA-IR) was calculated by the formula: HOMA-IR=fasting insulin concentrations (mIU/L)×fasting glucose concentrations (mmol/L)/22.5.14 eGFR was calculated using the abbreviated modification of diet in renal disease (MDRD) formula recalibrated for the Chinese: eGFR=186×(serum creatinine×0.011)−1.154× (age)−0.203×(0.742 if female)×1.233, where serum creatinine is expressed in μmol/L and 1.233 is the adjusting coefficient for the Chinese.17

A single, first-morning-void urine sample was collected for the measurement of urinary albumin and creatinine. Urinary albumin concentration was measured by an immunoturbidimetric method (Beijing Atom High-Tech, Beijing, China) and urinary creatinine concentration was determined by Jaffe's kinetic method on an autoanalyzer (Hitachi 7600-020, Tokyo, Japan). Urinary ACR was calculated as milligrams of urinary albumin excretion per gram of urinary creatinine. Those with the highest quartile of urinary ACR were classified as having low-grade albuminuria (ACR>6.10 mg/g in males and >8.76 mg/g in females).

Statistical analysis

All statistical analyses were conducted using SAS V.9.2 (SAS Institute Inc, Cary, North Carolina, USA). Continuous variables were presented as means±SDs or medians (IQRs) for skewed variables. Categorical variables were expressed as numbers (proportions).

Participants were categorised into five groups based on their HbA1c levels: ≤37 mmol/mol (5.5%), 38–42 mmol/mol (5.6–6.0%), 43–48 mmol/mol (6.1–6.5%), 49–53 mmol/mol (6.6–7.0%), and >53 mmol/mol (7.0%). Differences among the five groups were analysed by one-way analysis of variance (ANOVA) for continuous variables and χ2 test for categorical variables. The p values for trend were tested using linear regression and the Cochran-Mantel-Haenszel (CMH) method for continuous and categorical variables, respectively. Associations of urinary ACR levels with each 1-SD increase in glycaemic indicators (ie, FPG, 2 h PG and HbA1c) were examined using linear regression models at different levels of adjustment, with urinary ACR log-transformed before analyses.

Logistic regression models were used to evaluate the association between HbA1c levels and low-grade albuminuria. The risk of low-grade albuminuria in relation to each 5.5 mmol/mol (0.5%) increment of HbA1c was further explored in subgroups stratified by age (≥65/<65 years), sex (male/female), physical activity (low/moderate/high), current smoker (yes/no), current drinker (yes/no), obesity (yes/no), diabetes (yes/no), duration of diabetes (≥5/<5 years), use of antidiabetic drugs (yes/no), and use of ACE inhibitor (ACEI)/angiotensin receptor blocker (ARB) drugs (yes/no) with adjustment for potential confounding factors.

Significant tests were two tailed and a p value <0.05 was considered statistically significant.


Characteristics of participants

Table 1 shows demographic and cardiometabolic characteristics of the study population by HbA1c categories. In groups with higher HbA1c levels, participants were older, less likely to be physically active, and had higher levels of BMI, SBP, DBP, TG, LDL-c, FPG, 2 h PG and HOMA-IR as well as increased prevalences of obesity, hypertension and diabetes, but lower levels of HDL-c and education (all pfor trend <0.0001). In addition, 5.4% of study participants were taking ACEI/ARB medications and 6.2% of participants were using antidiabetic drugs. Generally, the proportions increased with elevated HbA1c levels (pfor trend <0.0001). The mean duration of diabetes in previously diagnosed diabetes was 4.9 years.

Table 1

General characteristics of study participants by HbA1c levels

Urinary ACR increased significantly with increasing HbA1c levels (pfor trend <0.0001; table 1). Likewise, a significant increase in the prevalence of low-grade albuminuria was observed according to different HbA1c levels (21.3%, 23.9%, 29.2%, 35.6% and 47.5% in HbA1c categories of ≤37 mmol/mol (5.5%), 38–42 mmol/mol (5.6–6.0%), 43–48 mmol/mol (6.1–6.5%), 49–53 mmol/mol (6.6–7.0%) and >53 mmol/mol (7.0%), respectively; pfor trend <0.0001, figure 1).

Figure 1

Prevalence of low-grade albuminuria according to different glycated haemoglobin A1c (HbA1c) levels. p Values were calculated using the χ2 test.

Associations between urinary ACR and glycaemic indicators

The univariate analysis revealed that urinary ACR was significantly associated with HbA1c, FPG and 2 h PG. The associations remained significant after adjustment for confounding factors such as age, sex, smoking and drinking status, physical activity, BMI, SBP, lipids, medications and eGFR (all p <0.0001). Further adjustment for HbA1c levels reduced the associations of ACR with FPG and 2 h PG to null. HbA1c was still significantly associated with ACR after further adjustment for FPG and 2 h PG (p<0.0001, table 2).

Table 2

Associations of urinary ACR with each 1-SD increase in FPG, 2 h PG and HbA1c

Associations between low-grade albuminuria and HbA1c

Using participants with HbA1c≤37 mmol/mol (5.5%) as the reference group, a dose–response relationship was found between HbA1c levels and risks of low-grade albuminuria (table 3). Although the magnitude of association was decreased after adjustment for confounding factors, it remained significant, especially for groups with higher HbA1c levels. Further adjustment for FPG and 2 h PG caused little change and the dose–response relationship still remained (ORs were 1.00, 1.05, 1.25, 1.40 and 2.21 for HbA1c categories of ≤37 mmol/mol (5.5%), 38–42 mmol/mol (5.6–6.0%), 43–48 mmol/mol (6.1–6.5%), 49–53 mmol/mol (6.6–7.0%) and >53 mmol/mol (7.0%), respectively; pfor trend <0.0001, table 3). The association between low-grade albuminuria and HbA1c was fairly consistent among subgroups (p values for interaction >0.05), except for the subgroup of obesity (p value for interaction=0.033; figure 2). Moreover, the receiver operating characteristic curve (ROC) analysis revealed that the area under the curve was 0.58 (0.56–0.59) and the optimal cut-off value of HbA1c for low-grade albuminuria was 39 mmol/mol (5.7%) with the largest Youden Index.

Table 3

Associations of risks of low-grade albuminuria with HbA1c levels

Figure 2

Adjusted ORs of low-grade albuminuria associated with each 5.5 mmol/mol (0.5%) increase in HbA1c in subgroups. All models were adjusted for potential confounding factors including sex (except for sex strata), age, smoking status (except for current smoker strata), drinking status (except for current drinker strata), physical activity (except for physical activity strata), BMI, TG, HDL-c, LDL-c, SBP, use of ACEI/ARB drugs (except for use of ACEI/ARB drugs strata), use of antidiabetic drugs (except for use of antidiabetic drugs strata), eGFR, FPG and 2 h PG. aData were analysed with further adjustment for duration of diabetes. bData were analysed in individuals with diabetes, including those newly diagnosed and those who have been previously diagnosed. (2 h PG, 2 h postload glucose; ACEI, ACE inhibitor; ARB, angiotensin receptor blocker; BMI, body mass index; FPG, fasting plasma glucose; eGFR, estimated glomerular filtration rate; HbA1c, glycated haemoglobin A1c; HDL-c, high-density lipoprotein cholesterol; LDL-c, low-density lipoprotein cholesterol; SBP, systolic blood pressure; TG, triglyceride).


In this study, we found a significant association between HbA1c levels and risks of low-grade albuminuria, after controlling for confounding factors including FPG and 2 h PG. In contrast, no significant associations were detected between FPG, 2 h PG and urinary ACR levels after adjustment for HbA1c.

Previous studies have already reported the associations between FPG, 2 h PG, HbA1c and low-grade albuminuria. Sung et al18 showed that FPG was closely related to low-grade albuminuria in the overall study population, but for persons without diabetes and those without hypertension, the association no longer existed. Nevertheless, this study might be the first to evaluate and compare associations of all three glycaemic markers with low-grade albuminuria in a general Chinese population with adjustment for conventional cardiovascular risk factors.

Previous studies have demonstrated a positive association between HbA1c and microalbuminuria.19 ,20 In persons with diabetes, Krolewski et al19 reported an HbA1c threshold of 87 mmol/mol (10.1%), above which the risk of microalbuminuria rose steeply. Additionally, among patients with type 2 diabetes, the risk of microalbuminuria was also closely related to HbA1c levels in Southern India.20 However, associations between microalbuminuria and HbA1c were studied mainly in patients with diabetes, as well as in studies with small sample sizes. Therefore, the generalisability of the results was limited. In this context, this study was designed to use a general community-based population to include both individuals with diabetes and individuals without diabetes. We found that participants with higher HbA1c levels were more likely to have combined microalbuminuria and macroalbuminuria in Chinese adults (data not shown), which was in agreement with previous studies. Moreover, we found a positive association between glycaemic markers and low-grade albuminuria, which is an earlier predictive factor of cardiovascular diseases.6–8 ,21–23 A retrospective analysis revealed that any degrees of urinary albuminuria were risk factors of cardiovascular diseases.2 The Framingham Heart Study showed that there were sex-specific thresholds of urinary ACR (3.9 mg/g for males and 7.5 mg/g for females) for an increased incidence of cardiovascular events and mortality.6 In a community-based sample of elderly individuals in Europe, low-grade albuminuria was a marker for subclinical cardiovascular damage, which predisposed heart failure in the future.7 Moreover, the Strong Heart Study conducted in American Indians also reported that albuminuria within the ‘normal’ range (<30 mg/g) predicted cardiovascular diseases.23

Associations between glycaemic markers and cardiovascular disease predictors or cardiovascular diseases were extensively investigated in the literature.24–28 The Atherosclerosis Risk in Communities (ARIC) study found that elevated HbA1c, but not FPG, was associated with the risk of cardiovascular events in individuals without diabetes.25 The Strong Heart Study reported that increases in FPG and HbA1c were associated with increased risks of cardiovascular diseases.26 Moreover, Huang et al28 assessed the associations of HbA1c, FPG and 2 h PG with carotid intima-media thickness (CIMT) in respondents with normal glucose tolerance. The authors found that higher HbA1c levels were significantly associated with elevated CIMT independent of conventional cardiovascular risk factors, whereas FPG and 2 h PG were not associated with CIMT. Similar results were found in this study, with an independent association between HbA1c and low-grade albuminuria, and a lack of independent correlation of urinary ACR with FPG and 2 h PG.

Additionally, since the duration of diabetes is a very important confounder for the association between HbA1c and low-grade albuminuria, it was taken into consideration by adjustment when estimating the OR as well as by stratification on this variable (duration of diabetes ≥5 and <5 years) in individuals with diabetes. The association between HbA1c levels and risks of low-grade albuminuria in diabetes remained significant after the adjustment as well as in different strata of diabetes duration. Similarly, to consider the important role of antidiabetic and ACEI/ARB drugs in the association, subgroup analyses in participants without antidiabetic or ACEI/ARB medications were also conducted with significant findings. Moreover, the association of HbA1c levels with low-grade albuminuria among other subgroups was largely consistent, except that it differed between participants with and without obesity. The potential modifying effect of obesity status might be due to its impact on the development of albuminuria as well as on glucose homoeostasis.29 ,30

HbA1c values reflect average blood glucose levels including postprandial plasma glucose spikes over the past 2–3 months, and have low variability, particularly in individuals without diabetes.31 ,32 These characteristics may contribute to the superiority of HbA1c over FPG, which primarily reflects hepatic glucose output at the time of sampling, in predicting long-term macrovascular diseases as well as low-grade albuminuria and other early cardiovascular disease predictors. In addition, the larger variability of 2 h PG relative to FPG and HbA1c demonstrated by Selvin et al31 may result in a lack of significant association between 2 h PG and urinary ACR, although 2 h PG was reported to be associated with cardiovascular diseases in patients with diabetes.33

The potential pathophysiological mechanism linking HbA1c concentrations and low-grade albuminuria might involve several different pathways. Among individuals with HbA1c levels <48 mmol/mol (6.5%), insulin resistance could result in changes in the amount and/or the effects of nitric oxide and other chemical mediators by causing endothelial dysfunction.34–36 The dysfunction of the glomerular capillary wall might lead to increased leakage of albuminuria at the glomerulus.37 For individuals with high HbA1c levels, elevated albuminuria was most likely caused by chronic hyperglycaemia, which resulted in loss of charge of selectivity and glomerular hyperperfusion and hyperfiltration accompanied by glycation of basement membrane proteins.38–40

Several limitations of our study should be mentioned. First, owing to the cross-sectional characteristic of this study, we draw no conclusion about cause and effect between HbA1c concentrations and low-grade albuminuria. Second, urinary albumin excretion was examined at a single time. Previous studies have shown that a 24 h urine sample or three samples from three consecutive days would yield results of more stable urine albumin excretion.41 Nevertheless, it was reported that use of spot urine could be a reliable alternative to 24 h urinary albumin excretion due to its good agreement with 24 h urinary albumin excretion, as well as the convenience of large epidemiological studies.42 ,43

In conclusion, our study demonstrated that elevated HbA1c concentrations were associated with increased risks of low-grade albuminuria and might have superiority over FPG and 2 h PG in the middle-aged and elderly Chinese. Prospective studies are warranted to determine the role of HbA1c in the development of low-grade albuminuria and compare the association to that of FPG and 2 h PG with low-grade albuminuria. Whether interventional measures to decrease HbA1c levels were associated with reduced risks of low-grade albuminuria is also a matter of interest.


The authors thank all the participants for their contribution and participation.


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  • XH and YZ contributed equally.

  • Contributors XH, YZ, YX and GN contributed to the conception and design of this study. XH, YZ, BX, WS, LL and JS were involved in the acquisition of data. XH and YZ conducted the statistical analysis and interpretation of data, and drafted the manuscript. MX, JL, YB, WW, YX and GN contributed to the revision of the manuscript for important intellectual content. All authors read and approved the final manuscript.

  • Funding This work is supported by grants from the Key Laboratory for Endocrine and Metabolic Diseases of Ministry of Health (1994DP131044), the National Clinical Research Center for Metabolic Diseases of Ministry of Health (2013BAI09B13), the National Key New Drug Creation and Manufacturing Program of Ministry of Science and Technology (2012ZX09303006–001), the National High Technology Research and Development Program of China (863 Program, 2012AA02A509), and the National Natural Science Foundation of China (81100564, 81321001, 81030011, 81130016, 81222008, and 81270877).

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval The study protocol was approved by the Institutional Review Board of the Rui-Jin Hospital and was in accordance with the principle of Helsinki Declaration II.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement The data set for this study is available on request from the corresponding author.

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